数字农科院2.0

Characterization of the Endophytic Bacillus subtilis KRS015 Strain for Its Biocontrol Efficacy Against Verticillium dahliae

文献类型： 外文期刊

作者： Song, Jian;Wang, Dan;Han, Dongfei;Zhang, Dan-Dan;Li, Ran;Kong, Zhi-Qiang;Dai, Xiao-Feng;Subbarao, Krishna V.;Chen, Jie-Yin

作者机构：

关键词： Bacillus subtilis;biological control;plant growth promotion;plant immunity;Verticillium dahliae;Verticillium wilt

期刊名称： PHYTOPATHOLOGY

ISSN： 0031-949X

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： Endophytes play important roles in promoting plant growth and controlling plant diseases. Verticillium wilt is a vascular wilt disease caused by Verticillium dahliae, a widely distributed soilborne pathogen that causes significant economic losses on cotton each year. In this study, an endophyte KRS015, isolated from the seed of the Verticillium wilt-resistant Gossypium hirsutum 'Zhongzhimian No. 2', was identified as Bacillus subtilis by morphological, phylogenetic, physiological, and biochemical analyses. The volatile organic compounds (VOCs) produced by KRS015 or its cell-free fermentation extract had significant antagonistic effects on various pathogenic fungi, including V. dahliae. KRS015 reduced Verticillium wilt index and colonization of V. dahliae in treated cotton seedlings significantly; the disease reduction rate was similar to 62%. KRS015 also promoted plant growth, potentially mediated by the growth-related cotton genes GhACL5 and GhCPD-3. The cell-free fermentation extract of KRS015 triggered a hypersensitivity response, including reactive oxygen species (ROS) and expression of resistance-related plant genes. VOCs from KRS015 also inhibited germination of conidia and the mycelial growth of V. dahliae, and were mediated by growth and development-related genes such as VdHapX, VdMcm1, Vdpf, and Vel1. These results suggest that KRS015 is a potential agent for controlling Verticillium wilt and promoting growth of cotton.

分类号：

  • 相关文献

[1]Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae. Yujia Shan,Dan Wang,Fu Hua Zhao,Jian Song,He Zhu,Yue Li,Xiao Jun Zhang,Xiao Feng Dai,Dongfei Han,Jie Yin Chen. 2024

[2]The potential of Burkholderia gladioli KRS027 in plant growth promotion and biocontrol against Verticillium dahliae revealed by dual transcriptome of pathogen and host. Dan Wang,Haiping Lin,Yujia Shan,Jian Song,Dan Dan Zhang,Xiao Feng Dai,Dongfei Han,Jie Yin Chen. 2024

[3]Insights into the Biocontrol Function of a Burkholderia gladioli Strain against Botrytis cinerea. Wang, Dan,Luo, Wan-Zhen,Zhang, Dan-Dan,Li, Ran,Kong, Zhi-Qiang,Song, Jian,Dai, Xiao-Feng,Alkan, Noam,Chen, Jie-Yin. 2023

[4]Bacillus megaterium A6 suppresses Sclerotinia sclerotiorum on oilseed rape in the field and promotes oilseed rape growth. Hu, Xiaojia,Xie, Lihua,Yu, Changbing,Li, Yinshui,Zhang, Shujie,Liao, Xing,Roberts, Daniel P.,Maul, Jude E.. 2013

[5]Comparative Genomics Assisted Functional Characterization of Rahnella aceris ZF458 as a Novel Plant Growth Promoting Rhizobacterium. Xu S.,Zhao Y.,Peng Y.,Shi Y.,Xie X.,Chai A.,Li B.,Li L.. 2022

[6]Molecular cloning and characterization of enhanced disease susceptibility 1 (EDS1) from Gossypium barbadense. Su, Xiaofeng,Qi, Xiliang,Cheng, Hongmei.

[7]Identification of long non-coding RNAs in Verticillium dahliae following inoculation of cotton. Ran Li,Hui Shan Xue,Dan Dan Zhang,Dan Wang,Jian Song,Krishna V. Subbarao,Steven J. Klosterman,Jie Yin Chen,Xiao Feng Dai. 2022

[8]Interactions between Verticillium dahliae and cotton: pathogenic mechanism and cotton resistance mechanism to Verticillium wilt. Zhu, Yutao,Zhao, Mei,Li, Taotao,Wang, Lianzhe,Liao, Chunli,Liu, Dongxiao,Zhang, Huamin,Zhao, Yanpeng,Liu, Lisen,Ge, Xiaoyang,Li, Bingbing. 2023

[9]Analysis of the PRA1 Genes in Cotton Identifies the Role of GhPRA1.B1-1A in Verticillium dahliae Resistance. Na Wu,Wen Jie Li,Chen Chen,Yan Peng Zhao,Yu Xia Hou. 2022

[10]A Verticillium dahliae exoglucanase as potential HIGS target interacts with a cotton cysteine protease to confer resistance to cotton Verticillium wilt. Su, Xiaofeng,Wang, Qi,Zhang, Tao,Ge, Xiaoyang,Liu, Wende,Guo, Huiming,Wang, Xingfen,Sun, Zhengwen,Li, Zhiqiang,Cheng, Hongmei. 2024

[11]The secretome of Verticillium dahliae in collusion with plant defence responses modulates Verticillium wilt symptoms. Zhang, Dan-Dan,Dai, Xiao-Feng,Klosterman, Steven J.,Subbarao, Krishna, V,Chen, Jie-Yin. 2022

[12]Verticillium dahliae Effector VdCE11 Contributes to Virulence by Promoting Accumulation and Activity of the Aspartic Protease GhAP1 from Cotton. Li, Chi,Qin, Jun,Huang, Yingqi,Shang, Wenjing,Chen, Jieyin,Klosterman, Steven J.,Subbarao, Krishna V.,Hu, Xiaoping. 2023

[13]New insights into defense responses against Verticillium dahliae infection revealed by a quantitative proteomic analysis in Arabidopsis thaliana. Wu, Min,Li, Qiulin,Xia, Guixian,Zhang, Yongshan,Wang, Fuxin. 2022

[14]A glycine-rich nuclear effector VdCE51 of Verticillium dahliae suppresses plant immune responses by inhibiting the accumulation of GhTRXH2. Li, Chi,Huang, Yingqi,Shang, Wenjing,Chen, Jieyin,Klosterman, Steven J.,Subbarao, Krishna, V,Qin, Jun,Hu, Xiaoping. 2024

[15]Genome Resource of Bacillus subtilis KRS015, a Potential Biocontrol Agent for Verticillium dahliae. Song, Jian,Chen, Jie-Yin,Zhang, Dan-Dan,Li, Ran,Kong, Zhi-Qiang,Zhu, He,Dai, Xiao-Feng,Han, Dongfei,Wang, Dan. 2024

[16]Evaluation of the effectiveness of a consortium of three plant-growth promoting rhizobacteria for biocontrol of cotton Verticillium wilt. Yang, Wei,Luo, Yu-Ming,Yang, Wei,Zheng, Li,Liu, Hong-Xia,Yu, Yi-Yang,Guo, Jian-Hua,Zheng, Li,Wang, Kun-Bo. 2014

[17]Diversity of Endophytic Fungi from Different Verticillium-Wilt-Resistant Gossypium hirsutum and Evaluation of Antifungal Activity Against Verticillium dahliae In Vitro. Wang, Ling-Fei,Feng, Zi-Li,Zhao, Li-Hong,Shi, Yong-Qiang,Zhu, He-Qin.

[18]Unlocking antagonistic potential of Bacillus amyloliquefaciens KRS005 to control gray mold. Hong Yue Qi,Dan Wang,Dongfei Han,Jian Song,Muhammad Ali,Xiao Feng Dai,Xiao Jun Zhang,Jie Yin Chen. 2023

[19]Formulations of Bacillus subtilis BY-2 suppress Sclerotinia sclerotiorum on oilseed rape in the field. Hu, Xiaojia,Xie, Lihua,Yu, Changbing,Li, Yinshui,Jiang, Mulan,Liao, Xiangsheng,Che, Zhi,Liao, Xing,Roberts, Daniel P.,Maul, Jude E.. 2014

[20]Formulations of the endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiorum on oilseed rape and improve plant vigor in field trials conducted at separate locations. Roberts, Daniel P.,Maul, Jude E.,Emche, Sarah E.,McKenna, Laurie F.,Buyer, Jeffrey S.,Hu, Xiaojia,Liao, Xing,Guo, Xuelan,Liu, Yeying,Liu, Shengyi. 2011

作者其他论文 更多>>