数字农科院2.0

Revealing the formation of aged aroma in raw Pu-erh tea during the storage through comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry and molecular docking

文献类型： 外文期刊

作者： Bingsong Ma;Cunqiang Ma;Binxing Zhou;Shujing Liu;Jing Zhuang;Zhihui Feng;Junfeng Yin;Xinghui Li

作者机构：

关键词： Aged aroma;GC×GC-TOFMS;Molecular docking;Stored tea;Woody

期刊名称： Current Research in Food Science

ISSN： 2665-9271

年卷期： 2025 年 10 卷

页码：

收录情况： SCIE（2025版）

摘要： Pu-erh tea undergoes significant flavor transformations during long-term natural storage. This study systematically examined changes in quality components and characteristic attributes of raw Pu-erh tea (RaPT) various storage stages through extensive sampling. The total contents of tea polyphenols including six catechins (i.e. C, EC, EGC, GC, ECG and EGCG), free amino acids decreased, while the total flavonoid content increased over time. Additionally, 363 volatile organic compounds (VOCs) were identified using HS-SPME/GC × GC-TOFMS. Multivariate statistical analysis, combined with quantitative descriptive analysis (QDA), revealed progressive changes in aroma profiles throughout RaPT storage. Based on these dynamic changes, RaPT storage duration was classified into three distinct stages. Thirty aged characteristic aroma compounds, predominantly associated with woody odor, were identified through Pearson's correlation analysis. Furthermore, by integrating molecular docking technology and aroma recombination experiments, 18 key VOCs responsible for the aged aroma characteristics of RaPT were confirmed. These findings elucidate their interaction mechanisms with olfactory receptors (ORs), providing new insights into the molecular basis of RaPT aroma evolution.

分类号：

  • 相关文献

[1]Quantitative descriptive analysis, non-targeted metabolomics and molecular docking reveal the dynamic aging and taste formation mechanism in raw Pu-erh tea during the storage. Bingsong Ma,Cunqiang Ma,Binxing Zhou,Xuan Chen,Yuhua Wang,Yifan Li,Junfeng Yin,Xinghui Li. 2025

[2]亚洲玉米螟雌蛾产卵偏好寄主植物的筛选及对葎草挥发性化学成分的电生理反应. 张文璐,王文强,白树雄,张天涛,何康来,王振营. 2018

[3]Insight into aroma dynamic changes during the whole manufacturing process of chestnut-like aroma green tea by combining GC-E-Nose, GC-IMS, and GC × GC-TOFMS. Yanqin Yang,Michael C. Qian,Yuliang Deng,Haibo Yuan,Yongwen Jiang. 2022

[4]Development of image similarity strategy based on targeted filtration for non-targeted HS-SPME/GC × GC fingerprints of volatile oils from Chinese patent medicines: A case of Chaihu Shugan Wan. Min He,Xinyue Yang,Yaping Li,Xuhuai Luo,Zhijian Tan,Sifan Luo. 2023

[5]Comprehensive investigation on the dynamic changes of volatile metabolites in fresh scent green tea during processing by GC-E-Nose, GC–MS, and GC × GC-TOFMS. Qiwei Wang,Jialing Xie,Lilei Wang,Yongwen Jiang,Yuliang Deng,Jiayi Zhu,Haibo Yuan,Yanqin Yang. 2024

[6]Functional analysis of female-biased odorant binding protein 6 for volatile and nonvolatile host compounds in Adelphocoris lineolatus (Goeze). Sun, L.,Wang, Q.,Wang, Q.,Khashaveh, A.,Zhang, Y. -J.,Guo, Y. -Y.,Wang, Q.,Yang, S.,Zhang, Z..

[7]Specific involvement of two amino acid residues in cis-nerolidol binding to odorant-binding protein 5 AlinOBP5 in the alfalfa plant bug, Adelphocoris lineolatus (Goeze). Gu, S. -H.,Guo, Y. -Y.,Zhang, Y. -J.,Han, L.,Zhou, J. -J.. 2013

[8]Investigation of the binding sites and orientation of caffeine on human serum albumin by surface-enhanced Raman scattering and molecular docking. Wang, Weinan,Duan, Yaokai,Jiang, Yong,Zhang, Liangren,Tu, Pengfei,Zhang, Wei,Zhao, Bing.

[9]Design, synthesis and biological evaluation of organophosphorous-homodimers as dual binding site acetylcholinesterase inhibitors. Xie, Ruliang,Zhao, Qianfei,Zhang, Tao,Mei, Xiangdong,Ning, Jun,Fang, Jing,Tang, Yun.

[10]Production of ACE inhibitory peptides from sweet sorghum grain protein using alcalase: Hydrolysis kinetic, purification and molecular docking study. Wu, Qiongying,Du, Jinjuan,Jia, Junqiang,Kuang, Cong,Wu, Qiongying,Jia, Junqiang.

[11]Functional and binding characterization of a single chain Fv antibody to abscisic acid and conjugated abscisic acid. Chen, Xiaojiao,Tan, Guiyu,Liu, Wei,Wang, Baomin,Zhang, Mingcai,Li, Zhaohu,Lu, Yao,Cao, Zhen.

[12]Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain. Wei, Jie,Zhang, Xiao,Xu, Dexin,Zhang, Guokun,Gao, Jun,Fan, Jungang,Zhou, Zhiquan.

[13]Experimental and molecular docking investigations on the inclusion mechanism of the complex of phloridzin and hydroxypropyl-beta-cyclodextrin. Zhang, Chun-Ling,Liu, Jie-Chao,Yang, Wen-Bo,Chen, Da-Lei,Jiao, Zhong-Gao.

[14]Ligand-binding properties of three odorant-binding proteins of the diamondback moth Plutella xylostella. Yuan Hai-bin,Zhu Jiao,Pelosi, Paolo,Liu Yang,Lin Ke-jian,Wang Gui-rong. 2016

[15]A novel angiotensin-I converting enzyme (ACE) inhibitory peptide from gastrointestinal protease hydrolysate of silkworm pupa (Bombyx mori) protein: Biochemical characterization and molecular docking study. Wu, Qiongying,Jia, Junqiang,Yan, Hui,Du, Jinjuan,Gui, Zhongzheng,Wu, Qiongying,Jia, Junqiang,Yan, Hui,Gui, Zhongzheng.

[16]Design, synthesis and bioactivity of novel phthalimide derivatives as acetylcholinesterase inhibitors. Zhang, Tao,Zhang, Lanxiang,Mei, Xiangdong,Dong, Mengya,Zhang, Kaixin,Ning, Jun.

[17]Purification and molecular docking study of a novel angiotensin-I converting enzyme (ACE) inhibitory peptide from alcalase hydrolysate of ultrasonic-pretreated silkworm pupa (Bombyx mori) protein. Jia, Junqiang,Wu, Qiongying,Yan, Hui,Gui, Zhongzheng,Jia, Junqiang,Wu, Qiongying,Yan, Hui,Gui, Zhongzheng. 2015

[18]Identification of borrelidin binding site on threonyl-tRNA synthetase. Zhang, Ji,Liu, Chongxi,Fang, Baozhu,Wang, Xiangjing,Xiang, Wensheng,Xiang, Wensheng.

[19]Systematic evaluation of chiral fungicide penflufen for the bioactivity improvement and input reduction using alphafold2 models and transcriptome sequencing. Kuan Fang,Jianwei Fang,Lingxi Han,Jijie Yin,Tong Liu,Xiuguo Wang. 2022

[20]Bitterness quantification and simulated taste mechanism of theasinensin A from tea. Jian Yong Zhang,Hong Chun Cui,Zhi Hui Feng,Wei Wei Wang,Yun Zhao,Yu Liang Deng,He Yuan Jiang,Jun Feng Yin,Ulrich H. Engelhardt. 2023

作者其他论文 更多>>