数字农科院2.0

Dual mutations containing E198A in β-tubulin eliminate the negative cross-resistance between carbendazim and diethofencarb in Corynespora cassiicola

文献类型： 外文期刊

作者： Jingkun Han;Jiamei Zhu;Jin Li;Dicheng Ma;Wei Mu;Hongqiang Dong;Feng Liu

作者机构：

关键词： Corynespora cassiicola;Cross-resistance;Molecular docking;Sensitivity profile

期刊名称： Pesticide Biochemistry and Physiology

ISSN： 0048-3575

年卷期： 2025 年 216 卷

页码：

收录情况： SCIE（2025版）

摘要： Corynespora cassiicola has been reported to develop severe resistant to most mainstream fungicides, particularly methyl benzimidazole carbamates (MBCs). Pathogens carrying the E198A mutation in β-Tubulin exhibit negative cross-resistance to N-phenyl carbamates. However, dual mutations involving E198A have been detected in MBCs-resistant isolates of C. cassiicola. Whether diethofencarb remains effective against C. cassiicola with dual mutations remains unclear and requires further investigation. Herein, resistance frequencies of C. cassiicola strains to carbendazim and diethofencarb were 100 % and 72.33 %, respectively. Dual mutations of E198A&M163I and E198A&F200S/C, which imposed fitness costs associated with increased membrane permeability, conferred multiple resistance to both carbendazim and diethofencarb. Molecular docking analysis indicated that these dual point mutations in β-TUBULIN changed the structure of binding pocket and weakened the binding affinity between β-TUBULIN and diethofencarb. Furthermore, sensitivity of C. cassiicola to fludioxonil, pyrimethanil, iprodione and prochloraz were evaluated to explore alternative fungicide options. The relatively stable sensitivity level of C. cassiicola to fludioxonil and prochloraz suggested their potential utility in Corynespora leaf spot (CLS) management. These findings enhanced our comprehension of the current resistance dynamics in C. cassiicola populations, and provided a crucial basis for guiding integrated CLS management strategies.

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