数字农科院2.0

CRISPR/Cas9-mediated ebony knockout causes melanin pigmentation and prevents moth Eclosion in Ectropis grisescens

文献类型： 外文期刊

作者： Jia Li Li;Shun Si Li;Zi Jun Luo;Ji Lu;Xiao Ming Cai;Zong Xiu Luo;Lei Bian;Chun Li Xiu;Nan Xia Fu;Nai Yong Liu;Zhao Qun Li

作者机构：

关键词： CRISPR/Cas9;Ebony;Ectropis grisescens;Lethal gene;Melanin pigmentation

期刊名称： Pesticide Biochemistry and Physiology

ISSN： 0048-3575

年卷期： 2024 年 200 卷

页码：

收录情况： SCIE（2024版）

摘要： Ectropis grisescens (Lepidoptera: Geometridae) is a destructive tea pest in China. Mimesis, characterized by changing body color, is an important trait of E. grisescens larvae. Hence, identifying melanin pathway-related genes may contribute to developing new pest control strategies. In the present study, we cloned Egebony, a gene potentially involved in melanin pigmentation in E. grisescens, and subsequently conducted CRISPR/Cas9-mediated targeted mutagenesis of Egebony to analyze its role in pigmentation and development. At the larvae, prepupae, and pupae stages, Egebony-knockout individuals exhibited darker pigmentation than the wild-type. However, Egebony knockout did not impact the colors of sclerotized appendants, including ocelli, setae, and claws. While mutant pupae could successfully develop into moths, they were unable to emerge from the puparium. Notably, embryo hatchability and larval survival of mutants remained normal. Further investigation indicated that mutant pupae exhibited significantly stronger shearing force than the wild-type, with the pigmented layer of mutant pupae appearing darker and thicker. Collectively, these results suggest that the loss of Egebony might increase the rigidity of the puparium and prevent moth eclosion. This study provides new insights into understanding the function and diversification of ebony in insect development and identifies a lethal gene that can be manipulated for developing effective pest control strategies.

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