数字农科院2.0

THP9 enhances seed protein content and nitrogen-use efficiency in maize

文献类型： 外文期刊

作者： Huang, Yongcai;Wang, Haihai;Zhu, Yidong;Huang, Xing;Li, Shuai;Wu, Xingguo;Zhao, Yao;Bao, Zhigui;Qin, Li;Jin, Yongbo;Cui, Yahui;Ma, Guangjin;Xiao, Qiao;Wang, Qiong;Wang, Jiechen;Yang, Xuerong;Liu, Hongjun;Lu, Xiaoduo;Larkins, Brian A.;Wa

作者机构：

期刊名称： NATURE

ISSN： 0028-0836

年卷期： 2022 年

页码：

收录情况： SCIE（2022版）

摘要： Teosinte, the wild ancestor of maize (Zea mays subsp. mays), has three times the seed protein content of most modern inbreds and hybrids, but the mechanisms that are responsible for thistrait are unknown(1,2). Here we use trio binning to create a contiguous haplotype DNA sequence of a teosinte (Zea mays subsp. parviglumis) and, through map-based cloning, identify a major high-protein quantitative trait locus, TEOSINTE HIGH PROTEIN 9 (THP9), on chromosome 9. THP9 encodes an asparagine synthetase 4 enzyme that is highly expressed in teosinte, but not in the B73 inbred, in which a deletion in the tenth intron of THP9-B73 causes incorrect splicing of THP9-B73 transcripts. Transgenic expression of THP9-teosinte in B73 significantly increased the seed protein content. Introgression of THP9-teosinte into modern maize inbreds and hybridsgreatly enhanced the accumulation of free amino acids, especially asparagine, throughout the plant, and increased seed protein content without affecting yield. THP9-teosinte seemsto increase nitrogen-use efficiency, which is important for promoting a high yield under low-nitrogen conditions.

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