数字农科院2.0

YTHDF3 recognizes DNA N6-methyladenine and recruits ALKBH1 for 6mA removal from genomic DNA

文献类型： 外文期刊

作者： Chen, Xin-Hui;Wang, Zi-Lu;Yang, Jincui;Chen, Min;Zhao, Si-Yi;Guo, Kun-Xiong;Zheng, Xuelong;Zhao, Zhengwei;Chen, Xiaoqiang;Li, Jing;Zhang, Min-Min;Ran, Ling;Zhu, Huifang;Gu, Xiao-Feng;Yan, Guang-Rong

作者机构：

关键词： Genomic DNA 6mA;ALKBH1;Genomic 6mA Reader;YTHDF3;dsDNA

期刊名称： EMBO JOURNAL

ISSN： 0261-4189

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： DNA N6-methyladenine (6mA) is an emerging epigenetic mark in the mammalian genome. ALKBH1 preferentially exhibits 6mA demethylase activity for single-stranded DNA (ssDNA) or bubbled/bulged DNA, but not for double-stranded DNA (dsDNA). Nevertheless, ALKBH1 significantly decreases the cellular 6mA level in genomic DNA, whose prevailing DNA conformation in living mammalian cells is dsDNA. Therefore, the demethylase activity of ALKBH1 toward 6mA in genomic DNA, especially dsDNA, remains largely debated. Here, we found that YTHDF3 increases the 6mA demethylase activity of ALKBH1 in genomic DNA with different conformations, including dsDNA. Compared with ALKBH1, YTHDF3 preferentially recognizes and binds to 6mA-modified DNA with different conformations. YTHDF3 recognizes 6mA in genomic DNA, and binds ALKBH1 to recruit it to sites near 6mA in genomic DNA, thereby facilitating the ALKBH1-mediated removal of 6mA in genomic dsDNA. In summary, YTHDF3 is a novel genomic DNA reader and guides ALKBH1 to remove 6mA in human genomic DNA.

分类号：

  • 相关文献

[1]基于FLUOstar平台的小麦dsDNA荧光定量与基因型分析. 肖永贵,Susanne DREISIGACKER,Claudia NU,EZ-RíOS,胡卫国,夏先春,何中虎. 2017

[2]Aptamer based ultrasensitive determination of the beta-adrenergic agonist ractopamine using PicoGreen as a fluorescent DNA probe. Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Huang, Yafei,Yan, Jiao.

[3]N6-methyladenosine modification of the mRNA for a key gene in purine nucleotide metabolism regulates virus proliferation in an insect vector. Zhu M.,Wu N.,Zhong J.,Chen C.,Liu W.,Ren Y.,Wang X.,Jin H.. 2024

作者其他论文 更多>>