数字农科院2.0

Detoxification of Insect-Derived Allergen PLA2 via Quercetin Modification: Molecular Simulation and Animal Validation

文献类型： 外文期刊

作者： Fukai Li;Liming Wu;Min Wang;Enning Zhou;Fei Pan;Jian Zhou;Mengrui Yang;Tongtong Wang;Liang Li;Qiangqiang Li

作者机构：

关键词： allergen;insect protein;mouse model;phospholipases A2;quercetin

期刊名称： Nutrients

ISSN： 2072-6643

年卷期： 2025 年 17 卷 17 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Insect-derived proteins constitute an underutilized biological resource requiring urgent exploration to address global food protein shortages. However, their widespread application is hindered by the allergenic potential, particularly phospholipase A2 (PLA2), a highly immunoreactive allergen prevalent in edible insects such as ants and honeybees. Objective: This study systematically investigated the molecular mechanism underlying quercetin-mediated reduction in PLA2 allergenicity, aiming to establish a novel strategy for developing hypoallergenic insect protein resources. Methods and Results: Through integrated computational and experimental approaches, we identified quercetin’s dual non-covalent and covalent binding capabilities with PLA2. Molecular docking revealed robust interactions (the binding energy of −6.49 kcal/mol) within the catalytic pocket. Meanwhile, mass spectrometry specifically identified Cys37 as the covalent modification site, which can bind to quercetin and increase the gyration radius (Rg) of PLA2 within 75–125 ns. Molecular dynamics simulations illustrated quercetin-induced conformational changes affecting critical antigenic epitopes. Murine experiments further confirmed that quercetin-modified PLA2 exhibited significantly reduced IgE reactivity and allergic responses compared to native PLA2, as demonstrated by assessments of anaphylactic behavior, histopathological changes, and measurements of serum IgE antibody and biogenic amine levels. Conclusions: Collectively, these findings provide a transformative approach to safely utilize insect-derived proteins for sustainable nutrition solutions.

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