数字农科院2.0

Surface display of Nocardia seriolae HRP1 on Bacillus subtilis and its application as live vaccine for largemouth bass

文献类型： 外文期刊

作者： Zhang, Fengli;Li, Ji-Tong;Zhang, Tengyuan;Wang, Xiao;Yao, Yuanyuan;Zhou, Zhigang

作者机构：

关键词： Nocardia seriolae;Micropterus salmoides;Oral vaccine;Surface display;Bacillus subtilis

期刊名称： SCIENTIFIC REPORTS

ISSN： 2045-2322

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Micropterus salmoides, adaptable and fast-growing, is a major farmed fish in China. Intensive farming causes multiple diseases, especially hard-to-treat nocardiosis. Vaccines are an effective and safe prevention and control strategy against fish diseases. In this experiment, the highly antigenic HRP1 gene of Nocardia seriolae (N. seriolae) was displayed on the surface of Bacillus subtilis (B. subtilis), and an oral vaccine (HRP1-cotC-B. subtilis) was successfully developed. Immune responses in inoculated fish were tested intermittently over 5 weeks post-administration. Immune protection was evaluated via challenge test. Serological parameters testing showed that the activity of lysozyme and Glutathione Peroxidase (GSH-Px) were significantly increased at the 7th and 21st day post-immunization, and the activity of Alkaline Phosphatase was extremely increased compared to the control. qRT-PCR detection found that oral vaccine could significantly boost the IgM expression of spleen and head-kidney. The expression of spleen major histocompatibility complex (MHC) was enhanced, MHCII at the 7th, 21st day and MHCI at 35th day post-immunization respectively. MHCI has an increasing trend in the head-kidney. CD8 increased in both spleen and head-kidney at different stages of immunization. The inflammatory cytokine il-1 beta and anti-inflammatory cytokine TGF-beta significantly increased in head-kidney at 35th day after immunization. The live bacterial vaccine altered the composition of the intestinal flora, demonstrated a decline in Firmicutes and an increase in Fusobacteria, and a significant decrease in Clostridium replaced by Cetobacterium at the genus level. Largemouth bass immunized with HRP1-cotC-B. subtilis spores exhibited a 18.18% relative survival rate after N. seriolae infection. In conclusion, this study developed a novel oral vaccine against N. seriolae in largemouth bass using B. subtilis spore surface display technology. Oral vaccination improves nonspecific immunity, induces innate and cellular immunity, strengthens bacterial resistance, and increases survival after pathogen infection. This offers an effective strategy for controlling fish nocardiosis in aquaculture.

分类号：

  • 相关文献

[1]Comparison and evaluation of DNA vaccines against Nocardia seriolae infection in largemouth bass ( Micropterus salmoides). Wang, Dandan,Zhang, Bo,Chen, Ming,Zeng, Hui,Zhang, Xu-Jie,Zhang, Yong-An,Zhou, Yang. 2025

[2]Construction of a cell-surface display system based on the N-terminal domain of ice nucleation protein and its application in identification of mycoplasma adhesion proteins. Bao, S.,Yu, S.,Zhang, F.,Sun, Y.,Tan, L.,Duan, Y.,Qiu, X.,Ding, C.,Bao, S.,Guo, X.,Lu, F..

[3]Immune Responses Induced by a Recombinant Lactiplantibacillus plantarum Surface-Displaying the gD Protein of Pseudorabies Virus. Assad Moon,Jingshan Huang,Xin Song,Tao Wang,Yanjin Wang,Yongfeng Li,Yuan Sun,Hongxia Wu,Huaji Qiu. 2024

[4]Immunogenic Responses Elicited by a Pool of Recombinant Lactiplantibacillus plantarum NC8 Strains Surface-Displaying Diverse African Swine Fever Antigens Administered via Different Immunization Routes in a Mouse Model. Assad Moon,Hongxia Wu,Tao Wang,Lian Feng Li,Yongfeng Li,Zhiqiang Xu,Jia Li,Yanjin Wang,Jingshan Huang,Tianqi Gao,Yuan Sun,Hua Ji Qiu. 2025

[5]Genetically modified rabies virus ERA strain is safe and induces long-lasting protective immune response in dogs after oral vaccination. Shuai, Lei,Wang, Xijun,Ge, Jinying,Wen, Zhiyuan,Chen, Weiye,Qin, Lide,Bu, Zhigao,Feng, Na,Xia, Xianzhu.

[6]Genetically modified rabies virus-vectored Ebola virus disease vaccines are safe and induce efficacious immune responses in mice and dogs. Shuai, Lei,Wang, Xijun,Wen, Zhiyuan,Ge, Jinying,Wang, Jinliang,Zhao, Dandan,Bu, Zhigao.

[7]A Lactococcus Lactis-Vectored Oral Vaccine Induces Protective Immunity Of Mice Against Enterotoxigenic Escherichia Coli Lethal Challenge. Song, JJ, Zhao, LY, Song, MX. 2020

[8]Pichia pastoris composition expressed aerolysin mutant of Aeromonas veronii as an oral vaccine evaluated in zebrafish (Danio rerio). Yao, Yuan-Yuan,Zhang, Qing-Shuang,Liu, Shu-Bin,Yang, Hong-Wei,Chen, Xing-Yu,Yang, Ya-Lin,Gao, Chen-Chen,Ran, Chao,Teame, Tsegay,Zhang, Zhen,Zhou, Zhi-Gang. 2024

[9]Rabies virus-based oral and inactivated vaccines protect minks against SARS-CoV-2 infection and transmission. Huo, Hong,Xiao, Shuang,Wang, Jinming,Wang, Xijun,Ge, Jinying,Zhong, Gongxun,Wen, Zhiyuan,Wang, Chong,Wang, Jinliang,Wang, Han,He, Xijun,Shuai, Lei,Bu, Zhigao. 2025

[10]Evaluation of safety and immunogenicity of a genetically modified rabies virus for use as an oral vaccine in several non-target species. Wang, Xijun,Huo, Hong,Shuai, Lei,Ge, Jinying,Peng, Liyan,Wang, Jinming,Xiao, Shuang,Chen, Weiye,Wen, Zhiyuan,Wang, Jinliang,Bu, Zhigao. 2024

[11]FoxO1 in Micropterus salmoides: Molecular characterization and its roles in glucose metabolism by glucose or insulin-glucose loading. Pei Chen,Xiufeng Wu,Xu Gu,Juan Han,Min Xue,Xiaofang Liang. 2021

[12]A new single-cell protein from Clostridium autoethanogenum as a functional protein for largemouth bass (Micropterus salmoides). Shifeng Ma,Xiaofang Liang,Pei Chen,Jie Wang,Xu Gu,Yuchang Qin,Christophe Blecker,Min Xue. 2022

[13]Proteomic analysis of hepatic fibrosis induced by a high starch diet in largemouth bass (Micropterus salmoides). Wu, Xiaoliang,Gu, Xu,Xue, Min,Ge, Chunyu,Liang, Xiaofang. 2022

[14]FoxO1 in Micropterus salmoides: Molecular characterization and its roles in glucose metabolism by glucose or insulin-glucose loading. Pei Chen,Xiufeng Wu,Xu Gu,Juan Han,Min Xue,Xiaofang Liang. 2021

[15]Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass (Micropterus salmoides) fed a cottonseed protein concentrate-based diet. Xiaoze Xie,Xiaofang Liang,Hao Wang,Qiang Zhu,Junjun Wang,Ying Chang,Eric Leclercq,Min Xue,Jie Wang. 2023

[16]Comparing effects of high starch diet or high lipid diet supplemented with different levels of zinc on intestinal barrier and microbe community in largemouth bass Micropterus salmoides. Bo Shi,Tong Qian,Ziyu Yin,Yu Zhang,Tianyu Feng,Zhiyong Dong,Wanjie Cai,Yuexing Zhang. 2024

[17]Evaluating the Impact of Arginine-to-Lysine Ratios on Growth Performance, Antioxidant Defense, and Immune Modulation in Juvenile Largemouth Bass (Micropterus salmoides). Yulong Sun,Shuailiang Zhang,Xueyao Luan,Tao Liu,Jiale He,Jiteng Wang,Tao Han. 2025

[18]Functional characterization of fatty acid desaturases and dietary lipid sources modulation of fatty acid conversion in largemouth bass (Micropterus salmoides). Yangyang Liu,Rudy Caparros Megido,Frédéric Francis,Jie Wang,Hao Wang,Liang Hu,Xiaofang Liang,Min Xue. 2026

[19]Growth, fatty acid composition, and fillet quality of largemouth bass (Micropterus salmoides) fed soybean oil and cottonseed oil diets. Yangyang Liu,Min Xue,Frédéric Francis,Rudy Caparros Megido,Jie Wang,Hao Wang,Xiaofang Liang. 2025

[20]Growth Enhancement and Disease Protection of Tobacco by the Volatile-producing Bacillus Subtilis Strain Tpb55. Zhang Cheng-sheng,Kong Fan-yu,Wang Jing. 2011

作者其他论文 更多>>