数字农科院2.0

RNAi effect in target and non-target pests correlates with the length of continuous matches in dsRNA sequences

文献类型： 外文期刊

作者： Zhaoyang Li;Junna Liu;Satyabrata Nanda;Zexin Zhong;Xuming Luo;Xuguo Zhou;Youjun Zhang;Chunxiao Yang;Huipeng Pan

作者机构：

关键词： Gene silencing;Henosepilachna vigintioctopunctata;Non-target effects;Propylaea japonica;RNA interference

期刊名称： Pesticide Biochemistry and Physiology

ISSN： 0048-3575

年卷期： 2025 年 210 卷

页码：

收录情况： SCIE（2025版）

摘要： RNA interference (RNAi) has emerged as a promising and environmentally friendly approach for controlling the pest Henosepilachna vigintioctopunctata (Hvig). Identifying lethal target genes in Hvig and evaluating the efficacy of oral dsRNA administration are crucial steps in this process. Additionally, assessing the potential risks of RNAi to non-target organisms (NTOs) is essential to ensure environmental safety. A soluble N-ethylmaleimide-sensitive factor attachment protein α (αSNAP) is an essential component of membrane fusion machinery, offering as a potential target gene for RNAi-based pest control. This study found that silencing of Hvαsnap with varying dsRNA concentrations (6.25, 12.5, 25, 50, 100, 200 ng/μL) induced 53.33 %–100 % lethality in Hvig, with an LC50 value of 10.15 ng/μL. Feeding the NTO, Propylaea japonica with dsHvαsnap or dsPjαsnap-1 containing 3–21-nt consecutive matches had no notable effects on survival, development, pupal weight, or gene expression. However, injecting these dsRNAs significantly increased P. japonica mortality. A chimeric dsGFP-αsnap-17-nt suppressed Hvαsnap expression and reduced Hvig larval survival but failed to induce RNAi in P. japonica. Overall, this study suggests that different species exhibit varying sensitivities to dsRNA, and increasing the number of consecutive matching bases may enhance RNAi effects in NTOs.

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