数字农科院2.0

Methylparaben suppresses the citrus postharvest pathogen Penicillium digitatum by undermining the cell wall and membrane and inducing the antioxidant system

文献类型： 外文期刊

作者： Fan Yang;Xiuying Zhong;Yuqing Wang;Juan Zhao;Yongqing Lu;Chao an Long

作者机构：

关键词： （0-1-4）Citrus;Inhibitory mechanism;Methylparaben;Penicillium digitatum

期刊名称： Scientia Horticulturae

ISSN： 0304-4238

年卷期： 2024 年 333 卷

页码：

收录情况： SCIE（2024版）

摘要： Parabens are one of the “generally regarded as safe” salts since its lower toxicity for the environment and human health. The curative activities of methylparaben (MP) had been detected previously by applying them into coating films in some fruits. In this study, we investigated the antifungal activities and putative mechanisms of MP against Penicillium digitatum, which causes the destructive citrus green mold disease during postharvest period. The results indicated that MP inhibited the P. digitatum hyphal growth in vitro at a minimum inhibitory/fungicidal concentration of 0.40 and 0.60 g L−1, respectively. The conidial germination rates were also distinctly restrained. Under scanning and transmission electron microscopies, the treated hyphae were slender and shrunken, had defects in developing conidiophores, and exhibited apoptotic mitochondria and vacuolated cytoplasmic matrix. Meanwhile, MP damaged the plasma membrane to accelerate the intracellular substances leaking out, and induced the activity of chitinase to degrade cell wall. The MP-treated hyphae showed activated the antioxidant enzymes, superoxide dismutase and peroxidase, and stimulated the content of antioxidant glutathione. MP alleviated the disease prevalence of inoculated citrus in the fruit decay test, but did not display good preservative effect in storage experiment. Collectively, our findings basically elucidate the underlying physiological antifungal mechanism of MP against P. digitatum.

分类号：

  • 相关文献

[1]Pullulan-based coatings carrying biocontrol yeast mixed with NaCl to control citrus postharvest disease decays. Juan Zhao,Yumei Wang,Qianyi Liu,Yuqing Wang,Chao an Long. 2024

[2]Ras-associated protein PdRSR1 is essential for sterol synthesis and stress tolerance to response to prochloraz in Penicillium digitatum. Fan Yang,Sirong Ji,Lanping Bai,Yan Ke,Weiqiang Lai,Chao An Long. 2025

[3]PdStuA Is a Key Transcription Factor Controlling Sporulation, Hydrophobicity, and Stress Tolerance in Penicillium digitatum. Yujie Du,Jinfan Zhu,Zhonghuan Tian,Chaoan Long. 2023

[4]Calcium disodium edetate controls citrus green mold by chelating Mn2+ and targeting pyruvate synthesis in the pathogen. Fan Yang,Shuqi Liu,Zhonghuan Tian,Yujie Du,Deyao Zhang,Chao an Long. 2023

[5]Exploration of the antimicrobial activities of biocontrol agent Streptomyces strain h114 against Penicillium digitatum in citrus. Tian Z.,Du Y.,Lu Y.,Zhu J.,Long C.-A.. 2024

[6]Postharvest biocontrol ability and antagonistic mechanism of Bacillus altitudins h217 against Penicillium digitatum in citrus. Yongqing Lu,Shuqi Liu,Yuqing Wang,Fan Yang,Zhonghuan Tian,Chao an Long. 2024

[7]PdMesA regulates polar growth, cell wall integrity, and full virulence in Penicillium digitatum of citrus. Shuqi Liu,Xinying Liu,Weiqiang Lai,Yongqing Lu,Yuqing Wang,Chao an Long. 2024

[8]Functional analysis of potential fumiquinazoline biosynthetic gene clusters in Penicillium digitatum of citrus. Shuqi Liu,Yongqing Lu,Yuqing Wang,Wei Fang,Chao an Long. 2025

[9]Perillaldehyde controls citrus green mold by inhibiting the ribosome biogenesis of Penicillium digitatum and improving citrus disease resistance. Shuqi Liu,Yuxin Jiang,Jiancheng Sun,Fan Yang,Yuqing Wang,Yongqing Lu,Weiqiang Lai,Chao an Long. 2025

[10]Structure-based virtual screening of highly potent inhibitors of the nematode chitinase CeCht1. Wei Chen,Qi Chen,Ashutosh Kumar,Xi Jiang,Kam Y. J. Zhang,Qing Yang. 2021

[11]Structure-based virtual screening of highly potent inhibitors of the nematode chitinase CeCht1. Wei Chen,Qi Chen,Ashutosh Kumar,Xi Jiang,Kam Y. J. Zhang,Qing Yang. 2021

[12]High-throughput screening and investigation of the inhibitory mechanism of α-glucosidase inhibitors in teas using an affinity selection-mass spectrometry method. Jianjian Gao,Mengxue Zhou,Dan Chen,Jiye Xu,Zhe Wang,Jiakun Peng,Zhiyuan Lin,Shuai Yu,Zhi Lin,Weidong Dai. 2023

[13]The inhibition mechanism of quercetin targeting β-Lactamases OXA-98 based on molecular dynamics simulation. Yanan Yang,Jingcheng Song,Yue Gao,Dongxia Li,Xuenan Li,Yujia Liu. 2023

[14]Recent advances in glycoside hydrolase family 20 and 84 inhibitors: Structures, inhibitory mechanisms and biological activities. Jiang, Xi,Yang, Qing. 2024

[15]Exploring the mechanism and inhibitory effect of robinin on xanthine oxidase using multi-spectroscopy and molecular dynamics simulation methods. Mengyao Liu,Xinning Wu,Hualei Chen,Fei Pan,Wenqian Wang,Xiaoming Fang,Wenjun Peng,Xiangxin Li,Wenli Tian. 2024

[16]Comprehensive analyses of inhibition of the Chinese traditional food-packaging materials, Indocalamus latifolius leaves (Zongye) on Aspergillus flavus. Peng, Wenjing,Ren, Xiaoxu,Dai, Haiting,Tai, Bowen,Yang, Bolei,Wang, Gang,Li, Xu,Xing, Fuguo. 2024

[17]Research progress on dual pathways of plant extracts inhibiting advanced glycation end products and their gut microbiota-mediated mechanisms. Meng Yao Jin,Huan Luo,Xiang Fei Han,Li Feng Li,Hua Lei Chen,Tian Yu Hou,Ya Na Zhao,He Li. 2025

[18]Computational exploration and molecular dynamics simulations for investigating the potential inhibitory mechanism of amantadine on the ion channel activity of bovine viral diarrhea virus p7. Xiao Wang,Ziwei Liu,Daolai Zhang,Yulong Wu,Yongfeng Li,Xiaowei Chen. 2025

作者其他论文 更多>>