数字农科院2.0

Construction of watermelon mosaic virus-Beijing isolate infectious clone and study of the dynamic localization and accumulation of virus-encoded proteins

文献类型： 外文期刊

作者： Mengjiao Pan;Linhao Ge;Weifang Gao;Zhaolei Li;Xue Li;Yalin Chen;Mingxuan Jia;Minghong Lu;Shoulin Jiang;Hongguang Cui;Wenxing Liang;Yaqin Wang;Fangfang Li

作者机构：

关键词： Protein accumulation;Subcellular localization;Viral infections clone;Viral pathogenicity;Watermelon mosaic virus

期刊名称： Virology

ISSN： 0042-6822

年卷期： 2025 年 609 卷

页码：

收录情况： SCIE（2025版）

摘要： Potyvirus is the largest genus of plant RNA viruses, and the members in the genus are known to cause significant damage to a wide variety of crops. In this study, we performed small RNA (sRNA) deep sequencing for identification of potential virus (es) in collected cucumber leaves with mosaic symptoms from fields in Beijing. Through this high-throughput sequencing and subsequent PCR amplification, we obtained a complete viral genome sequence of 10,044 bp, which showed the highest similarity to the watermelon mosaic virus (WMV) isolate FBR04-37 and exhibited the typical characteristics of potyviruses in its genome organization. The obtained WMV isolate was designated as WMV-BJ. We then constructed the infectious clone of WMV-BJ, which can successfully infect six kinds of cucurbitaceous plants by agroinfiltration. Among these, Cucumis melo showed obvious symptoms such as shrinkage and mosaic compared to other cucurbitaceous plants infected by this virus infectious clone. To understand the biological function of WMV-BJ, we further analyzed the dynamic subcellular localization and protein accumulation of WMV-BJ encoded 11 viral proteins. The results showed the WMV-BJ-encoded proteins displayed diverse subcellular localizations, and most viral proteins were easily degraded after expression in plant cells. Together, the construction of the WMV-BJ infectious clone in this study provides a valuable tool for further exploring the biology of this virus and the interaction between WMV-BJ and host plant, and useful information for investigating the functions of WMV-encoded proteins.

分类号：

  • 相关文献

[1]Dynamic QTL Analysis of Rice Protein Content and Protein Index Using Recombinant Inbred Lines. Zheng, Leina,Zhang, Wenwei,Chen, Xingang,Ma, Jing,Chen, Weiwei,Zhao, Zhigang,Wan, Jianmin,Zhai, Huqu,Wan, Jianmin.

[2]Genetic analysis and fine mapping of Watermelon mosaic virus resistance gene in cucumber. Tian, Guili,Miao, Han,Yang, Yuhong,Zhou, Jian,Lu, Hongwei,Wang, Ye,Xie, Bingyan,Zhang, Shengping,Gu, Xingfang.

[3]Coinfection of cotton plants with watermelon mosaic virus and a novel polerovirus in china. Xiuling Yang,Min Du,Shupeng Li,Xueping Zhou. 2021

[4]Importance of the rice TCD9 encoding alpha subunit of chaperonin protein 60 (Cpn60 alpha) for the chloroplast development during the early leaf stage. Jiang, Quan,Mei, Jie,Gong, Xiao-Di,Lin, Dong-Zhi,Dong, Yan-Jun,Xu, Jian-Long,Zhang, Jian-Hui,Teng, Sheng. 2014

[5]Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents. Tan Xiao-li,Zheng Xiang-feng,Zhang Zhi-yan,Wang Zheng,Xia Heng-chuan,Gu Shou-lai,Lu Chang-ming. 2014

[6]Cloning and analysis of the soybean MEKK gene. Sha, A. -H.,Ba, H. -P.,Shan, Z. -H.,Chen, H. -F.,Chen, S. -L.,Qiu, D. -Z.,Zhou, X. -A.,Sha, A. -H.,Chen, Y. -H.. 2015

[7]An alfalfa (Medicago sativa L.) ethylene response factor gene, MsERF11, enhances salt tolerance in transgenic Arabidopsis. Chen, Tingting,Yang, Qingchuan,Ding, Wang,Chen, Tingting,Zhang, Xinquan,Gruber, Margaret. 2012

[8]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[9]The rice TCD11 encoding plastid ribosomal protein S6 is essential for chloroplast development at low temperature. Wang, Wen-Juan,Zheng, Kai-Lun,Gong, Xiao-Di,Lin, Dong-Zhi,Dong, Yan-Jun,Gong, Xiao-Di,Xu, Jian-Long,Xu, Jian-Long,Huang, Ji-Rong.

[10]Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.. Chao, Yuehui,Kang, Junmei,Yang, Qingchuan,Wu, Mingsheng,Li, Yan,Chao, Yuehui,Wang, Pingqing,Long, Ruicai,Qin, Zhihui,Sun, Yan. 2009

[11]Stability and localization of 14-3-3 proteins are involved in salt tolerance in Arabidopsis. Tan, Tinghong,Cai, Jingqing,Zhan, Erbao,Zhou, Huapeng,Tan, Tinghong,Cai, Jingqing,Zhan, Erbao,Zhou, Huapeng,Yang, Yongqing,Guo, Yan,Zhao, Jinfeng.

[12]Molecular cloning and characterization of a group 3 LEA gene from Agropyron mongolicum Keng. Zhao, Yan,Yun, Jinfeng,Shi, Fengmin,Wang, Junjie,Yang, Qingchuan,Chao, Yuehui. 2010

[13]Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Wang, Huan L.,Wang, Heng,Zhu, Zheng M.,Wang, Chen F.,Zhu, Meng J.,Mo, De L.,Yang, Shu L.,Li, Kui.

[14]Effect of UV-C Irradiation on Stilbene Synthase Localization in Young Grape Plants. Tang, K.,Fang, F.,Yang, H. R.,Huang, W. D.,Fang, F..

[15]Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity. Chen, Tingting,Sun, Yan,Chen, Tingting,Yang, Qingchuan,Kang, Junmei,Ding, Wang,Zhang, Tiejun,Chen, Tingting,Zhang, Xinquan,Gruber, Margaret.

[16]Isolation and characterization of GoRAV, a novel gene encoding a RAV-type protein in Galegae orientalis. Chen, Xiaofang,Wang, Zan,Wang, Xuemin,Gao, Hongwen,Chen, Xiaofang,Ren, Jizhou,Wang, Xuemin.

[17]Cloning and expression of MyoG gene from Hu sheep and identification of its myogenic specificity. Zhang, Zhentao,Xu, Feng,Zhang, Yani,Li, Wei,Yin, Yanhui,Zhu, Caiye,Elsayed, A. K.,Li, Bichun,Du, Lixin,Elsayed, A. K..

[18]Isolation and characterization of a gene encoding an ethylene responsive factor protein from Ceratoides arborescens. Dong, Jie,Wang, Xuemin,Wang, Zan,Gao, Hongwen,Wang, Kang.

[19]Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses. Wang, Lijuan,Ma, Fei,Shen, Wenbiao,Xu, Sheng,Wang, Ren,Zheng, Tianqing,Chen, Huiping,Chen, Huiping.

[20]Molecular characteristics of the porcine TIMD4 gene and its association analysis. Yuan, Jing,Tang, Zhong-Lin,Yang, Shulin,Li, Kui,Cao, Ji-Yue.

作者其他论文 更多>>