数字农科院2.0

GhMYB30-GhMUR3 affects fiber elongation and secondary wall thickening in cotton

文献类型： 外文期刊

作者： Wu, Aimin;Lian, Boying;Hao, Pengbo;Fu, Xiaokang;Zhang, Meng;Lu, Jianhua;Ma, Liang;Yu, Shuxun;Wei, Hengling;Wang, Hantao

作者机构：

关键词： upland cotton;GhMUR3;fiber elongation;secondary wall thickening;GhMYB30

期刊名称： PLANT JOURNAL

ISSN： 0960-7412

年卷期： 2023 年

页码：

收录情况： SCIE（2023版） ; ; EI（2023版）

摘要： Xyloglucan, an important hemicellulose, plays a crucial role in maintaining cell wall structure and cell elongation. However, the effects of xyloglucan on cotton fiber development are not well understood. GhMUR3 encodes a xyloglucan galactosyltransferase that is essential for xyloglucan synthesis and is highly expressed during fiber elongation. In this study, we report that GhMUR3 participates in cotton fiber development under the regulation of GhMYB30. Overexpression GhMUR3 affects the fiber elongation and cell wall thickening. Transcriptome showed that the expression of genes involved in secondary cell wall synthesis was prematurely activated in OE-MUR3 lines. In addition, GhMYB30 was identified as a key regulator of GhMUR3 by Y1H, Dual-Luc, and electrophoretic mobility shift assay (EMSA) assays. GhMYB30 directly bound the GhMUR3 promoter and activated GhMUR3 expression. Furthermore, DAP-seq of GhMYB30 was performed to identify its target genes in the whole genome. The results showed that many target genes were associated with fiber development, including cell wall synthesis-related genes, BR-related genes, reactive oxygen species pathway genes, and VLCFA synthesis genes. It was demonstrated that GhMYB30 may regulate fiber development through multiple pathways. Additionally, GhMYB46 was confirmed to be a target gene of GhMYB30 by EMSA, and GhMYB46 was significantly increased in GhMYB30-silenced lines, indicating that GhMYB30 inhibited GhMYB46 expression. Overall, these results revealed that GhMUR3 under the regulation of GhMYB30 and plays an essential role in cotton fiber elongation and secondary wall thickening. Additionally, GhMYB30 plays an important role in the regulation of fiber development and regulates fiber secondary wall synthesis by inhibiting the expression of GhMYB46.

分类号：

  • 相关文献

[1]Cotton GhMAX2 promotes single-celled fiber elongation by releasing the GhS1FA-mediated inhibition of fatty acid biosynthesis. Yaru Sun,Shuangxia Jin,Guoli Song. 2025

[2]Arabidopsis C3H14 and C3H15 have overlapping roles in the regulation of secondary wall thickening and anther development. Chai, Guohua,Zhu, Ming,Yu, Li,Qi, Guang,Tang, Xianfeng,Wang, Zengguang,Cao, Yingping,Yu, Changjiang,Zhou, Gongke,Kong, Yingzhen.

[3]R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time. Chai, Guohua,Wang, Zengguang,Tang, Xianfeng,Yu, Li,Qi, Guang,Wang, Dian,Yan, Xiaofei,Zhou, Gongke,Kong, Yingzhen.

[4]Transcriptome analysis reveals critical genes and key pathways for early cotton fiber elongation in Ligon lintless-1 mutant. Jing Sun,Liangyu Yao,Kang Liu,Youlu Yuan.

[5]Effects of fiber wax and cellulose content on colored cotton fiber quality. Zhaoe Pan,Donglei Sun,Junling Sun,Zhongli Zhou,Yinhua Jia,Baoyin Pang,Zhiying Ma,Xiongming Du.

[6]GhCaM7-like, a calcium sensor gene, influences cotton fiber elongation and biomass production. Cheng, Yuan,Lu, Lili,Yang, Zhaoen,Wu, Zhixia,Qin, Wenqiang,Yu, Daoqian,Ren, Zhongying,Li, Yi,Wang, Lingling,Li, Fuguang,Yang, Zuoren,Cheng, Yuan.

[7]PAG1, a cotton brassinosteroid catabolism gene, modulates fiber elongation. Zhang, Chaojun,Yang, Xiaojie,Liu, Kun,Wu, Zhixia,Zhang, Xueyan,Zheng, Wu,Liu, Chuanliang,Lu, Lili,Yang, Zhaoen,Qian, Yuyuan,Xu, Zhenzhen,Li, Changfeng,Li, Fuguang,Xun, Qingqing,Li, Jia.

[8]N-6-Methyladenosine mRNA modification regulates transcripts stability associated with cotton fiber elongation. Xing, Kun,Liu, Zhao,Liu, Le,Zhang, Jie,Qanmber, Ghulam,Wang, Ye,Liu, Lisen,Gu, Yu,Zhang, Changsheng,Li, Shuaijie,Zhang, Yan,Yang, Zuoren. 2023

[9]Arginine Decarboxylase Gene ADC2 Regulates Fiber Elongation in Cotton. Guangming Ren,Huijuan Mo,Ruqiang Xu. 2022

[10]Genome-wide analysis of the serine carboxypeptidase-like protein family reveals Ga09G1039 is involved in fiber elongation in cotton. Guoyong Fu,Baojun Chen,Xinxin Pei,Xiaoyang Wang,Xiao Wang,Mian Faisal Nazir,Jingjing Wang,Xiaomeng Zhang,Aishuang Xing,Zhaoe Pan,Zhongxu Lin,Zhen Peng,Shoupu He,Xiongming Du. 2023

[11]Sphingosine Promotes Fiber Early Elongation in Upland Cotton. Li Wang,Changyin Jin,Wenqing Zhang,Xueting Mei,Hang Yu,Man Wu,Wenfeng Pei,Jianjiang Ma,Bingbing Zhang,Ming Luo,Jiwen Yu. 2024

[12]Brassinosteroids in cotton: orchestrating fiber development. Qanmber, Ghulam,Liu, Zhao,Li, Fuguang,Yang, Zuoren. 2024

[13]Deciphering the dynamic expression network of fiber elongation and the functional role of the GhTUB5 gene for fiber length in cotton based on an introgression population of upland cotton. Ma, Jianjiang,Yang, Liupeng,Dang, Yuanyue,Shahzad, Kashif,Song, Jikun,Jia, Bing,Wang, Li,Feng, Juanjuan,Wang, Nuohan,Pei, Wenfeng,Wu, Man,Zhang, Xuexian,Zhang, Jinfa,Wu, Jianyong,Yu, Jiwen. 2025

[14]Harnessing the acid growth theory to optimize apoplastic acidification for enhancing cotton fiber elongation. Li, Cheng,Zameer, Roshan,Liu, Leidi,Wen, Qing,Zheng, Yuge,Zheng, Jurui,Yu, Chengde,Song, Guoli,Song, Chun-Peng,Li, Zhifang,Zou, Changsong. 2025

[15]Identification Of Tpx2 Gene Family I.n Upland Cotton And I ts Functional Analysis In Cotton Fiber Development. Zhang, Shuya,Zhang, Lipeng,Shi, Yuzhen,Jiang, Xiao,Ge, Qun,Gong, Wankui,Jia, Tingting,Li, Junwen,Shang, Haihong,Fan, Liqiang,Lei, Kang,Zhang, Zhibin,Zhen, Zhang,Deng, Xiaoying,Peng, Huo,Fan, Senmiao,Huang, Jinyong,Liu, Aiying,Shang, Haihong,Yuan, Youlu,Yuan, Youlu,Gong, Juwu,Zou, Xianyan. 2019

[16]Quantitative Trait Locus Mapping For P.lant Height And Branch N umber In An Upland Cotton Recombinant Inbred Line With An Snp-Based High-Density Genetic Map. Shi, Yuzhen,Xu, Aixia,Shang, Haihong,Liu, Aiying,Shang, Haihong,Yuan, Youlu,Zhang, Zhibin,Zou, Xianyan,Zhang, Zhen,Yuan, Youlu,Zhang, Zhen,Gong, Wankui,Gong, Juwu,Jiang, Xiao,Huang, Zhen,Fan, Senmiao,Ge, Qun,Deng, Xiaoying,Fan, Liqiang,Li, Junwen,Lei, Kang. 2019

[17]Genome Resequencing Reveals Genetic Variation B.etween The Parents Of A n Elite Hybrid Upland Cotton. Ma, Xiongfeng,He, Kunlun,Li, Wei,Pei, Xiaoyu,Ren, Zhongying,Song, Chengxiang,Liu, Yangai,Yang, Daigang,Sun, Kuan,Wang, Zhenyu,Song, Chengxiang,Zhang, Fei,Zhou, Xiaojian. 2018

[18]Association Mapping Analysis Of Fiber Y.ield And Quality Traits I n Upland Cotton (Gossypium Hirsutum L.). Ademe, Mulugeta Seyoum,Du, Xiongming,Li, Lin,Ma, Zhiying,Sun, Junling,Cai, Zhongmin,Wang, Liru,Zhu, Haiyong,Jia, Yinhua,He, Shoupu,Wang, Qinglian,Yang, Jinlong,Zhang, Jinbiao,Pang, Baoyin,Zhang, Xin,Liu, Jinhai,Qin, Hongde,Zhang, Xuelin,Zhou, Guanyin,Li, Zhikun,Huang, Aifen,Pan, Zhaoe,Yang, Jun,Liu, Hui,Yi, Xianda. 2017

[19]Genome-Wide Comparative Transcriptome Analysis Of C.ms-D2 And Its Maintainer A nd Restorer Lines In Upland Cotton. Zhang, Bingbing,Xing, Chaozhu,Zhang, Meng,Zhang, Jinfa,Zhang, Xuexian,Qi, Tingxiang,Guo, Liping,Wang, Hailin,Wu, Jianyong,Wu, Jianyong. 2017

[20]A High Density Slaf-Seq Snp G.enetic Map And Qtl F or Seed Size, Oil And Protein Content In Upland Cotton. Yang, Le,Wang, Wenwen,Teng, Zhonghua,Yang, Peng,Ali, Iftikhar,Zhang, Zhengsheng,Zhang, Jian,Sun, Ying,Liu, Dexin,Liu, Dajun,Guo, Kai,Liu, Fang,Ou, Yuncan,Ma, Junrui,Liu, Tianpeng,Cai, Xiaoyan. 2019

作者其他论文 更多>>