数字农科院2.0

Antibiotic Resistance of Escherichia coli and Klebsiella pneumoniae Isolated from Wild Raccoon Dogs in Shanghai, China

文献类型： 外文期刊

作者： Xiong, Xinhuo;Chen, Chen;Tao, Jin;Wei, Min;Wang, Xiaoyang;Wang, Chunmei;Ye, Wenchong;Zhou, Wen;Liu, Guoping;Zhang, Keyu

作者机构：

关键词： urban raccoon dogs;wild animals;Escherichia coli;Klebsiella pneumoniae;antimicrobial resistance;drug resistance genes

期刊名称： MICROBIAL DRUG RESISTANCE

ISSN： 1076-6294

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Wild animals may act as reservoirs for resistant bacteria, and resident bacteria carried by wild animals in cities may also be subject to anthropogenic pressures that affect their resistance. This study aimed to evaluate the antibiotic susceptibility and biofilm formation ability of Escherichia coli and Klebsiella pneumoniae isolated from wild raccoon dogs from Shanghai, China, and to identify the genes responsible for resistance to different classes of antibiotics. The horizontal transfer of resistant plasmids was assessed by plasmid conjugation assays and characterized by third-generation nanopore sequencing. E. coli and K. pneumoniae isolated from wild raccoon dogs in Shanghai had strong biofilm formation ability. They had high resistance rates to amoxicillin, co-trimoxazole, tetracycline, and other antibiotics, but they were still sensitive to advanced antibiotics. The isolates contained prevalent resistance genes and virulence genes, and plasmids could be transferred horizontally. The resistant plasmids are rich in gene transfer elements such as insertion sequences. This is the first description of the antimicrobial resistance status and genes of wild raccoon dogs in Shanghai. These results highlight the urgent need to understand the origin and spread of resistance genes in wild animals such as urban raccoon dogs in Shanghai.

分类号：

  • 相关文献

[1]Prevalent and Drug-Resistant Phenotypes and Genotypes of Escherichia coli Isolated from Healthy Cow’s Milk of Large-Scale Dairy Farms in China. Jiaojiao Gao,Yating Wu,Xianlan Ma,Xiaowei Xu,Aliya Tuerdi,Wei Shao,Nan Zheng,Yankun Zhao. 2025

[2]Molecular Epidemiology and Antimicrobial Resistance of Outbreaks of Klebsiella pneumoniae Clinical Mastitis in Chinese Dairy Farms. Fu, Shaodong,Wen, Chen,Wang, Zhenglei,Qiu, Yawei,Zhang, Yihao,Zuo, Jiakun,Xu, Yuanyuan,Han, Xiangan,Luo, Zhenhua,Chen, Wei,Miao, Jinfeng. 2022

[3]CHARACTERIZATION OF INTEGRON-MEDIATED ANTIMICROBIAL RESISTANCE AMONG ESCHERICHIA COLI STRAINS ISOLATED FROM A CAPTIVE POPULATION OF AMUR TIGERS IN CHINA. Xue, Yuan,Wang, Yulong,Zhang, Yanlong,Hua, Yuping,Chen, Jianfei,Liu, Dan. 2013

[4]Resistance of Strains Producing Extended-Spectrum beta-lactamases and Genotype Distribution Among Escherichia coli in China. Xue, Yuan,Hua, Yuping,Chen, Jianfei. 2012

[5]The emergence of multi-drug resistant and virulence gene carrying Escherichia coli strains in the dairy environment: a rising threat to the environment, animal, and public health. Shoaib, Muhammad,He, Zhoulin,Geng, Xiang,Tang, Minjia,Hao, Ruochen,Wang, Shengyi,Shang, Ruofeng,Wang, Xuehong,Zhang, Hongjuan,Pu, Wanxia. 2023

[6]Prevalence, Antimicrobial Susceptibility, and Molecular Characterization of Escherichia coli Isolated From Raw Milk in Dairy Herds in Northern China. Huimin Liu,Lu Meng,Lei Dong,Yangdong Zhang,Jiaqi Wang,Nan Zheng. 2021

[7]Antimicrobial drug resistance against Escherichia coli and its harmful effect on animal health. Arbab, Safia,Ullah, Hanif,Wang, Weiwei,Zhang, Jiyu. 2022

[8]The characterisation of antimicrobial resistant Escherichia coli from dairy calves. Merning Mwenifumbo,Adrian L. Cookson,Shengguo Zhao,Ahmed Fayaz,Jackie Benschop,Sara A. Burgess. 2023

[9]The prevalent dynamic and genetic characterization of mcr-1 encoding multidrug resistant Escherichia coli strains recovered from poultry in Hebei, China. Qing Wang,Weiwei Wang,Qiqi Zhu,Muhammad Shoaib,Wang Chengye,Zhen Zhu,Xiaojuan Wei,Yubin Bai,Jiyu Zhang. 2024

[10]Difference Analysis on Virulence Genes, Biofilms and Antimicrobial Susceptibility of Escherichia coli from Clinical and Subclinical Bovine Mastitis. Jiakun Zuo,Zhaoyang Lv,Liyan Lian,Zihao Wu,Shaodong Fu,Haiyang Zhang,Jing Wu,Zihao Pan,Yong Yu,Wei Chen,Wei Jiang,Huifang Yin,Zhaoguo Chen,Yunpeng Yi,Xiangan Han,Jinfeng Miao. 2025

[11]Novel ST3871 Escherichia coli exhibits diverse transmission modes, carrying the tet(X4) resistance gene. Wang, Qing,Wang, Chengye,Shoaib, Muhammad,Yao, Xiaohui,Chen, Xi,Qiu, Yanhua,Dai, Guonian,Lin, Honglin,Wang, Weiwei,Zhang, Jiyu. 2026

[12]一株大肠埃希菌烈性噬菌体ΦTRI-1的分离及特性分析. 高苗,杨金广,孙航军,刘旭,刘伟,王凤龙. 2015

[13]Editorial: Epidemiological investigations of zoonotic viruses and research on drugs and vaccines. Jingqiang Ren,Zhenzhen Zheng,Shen Yang,Xusheng Qiu,Mingxiao Ma. 2025

[14]The Klebsiella pneumoniae nitrogenase Fe protein gene (nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii. Cheng, Q,Day, A,Dowson-Day, M,Shen, GF,Dixon, R. 2005

[15]Complete genome sequence of endophytic nitrogen-fixing Klebsiella variicola strain DX120E. Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Wei, Chunyan,Li, Yangrui,Yang, Litao,Chen, Mingyue,Wang, Hongcheng,Li, Yuanyuan,An, Qianli,Yang, Litao. 2015

[16]A Small KPC-2-Producing Plasmid in Klebsiella pneumoniae: Implications for Diversified Vehicles of Carbapenem Resistance. Chen, Qiwei,Liu, Lizhang,Hu, Xiaofang,Jia, Xu,Gong, Xiaowei,Feng, Youjun,Huang, Man. 2022

[17]Co-infections of Klebsiella pneumoniae and Elizabethkingia miricola in black-spotted frogs (Pelophylax nigromaculatus). Shurui Li,Xiaoni Wang,Yulan Lu,Jiahuan Wang,Denghang Yu,Zhigang Zhou,Jin Wei,Lihe Liu,Jun Liu,Fuguo Liu,Yingbing Su,Hongsen Xu. 2023

[18]Construction and application of the conditionally essential gene knockdown library in Klebsiella pneumoniae to screen potential antimicrobial targets and virulence genes via Mobile-CRISPRi-seq. Qing Zhu,Qiang Lin,Yushan Jiang,Shuyan Chen,Junxuan Tian,Shijin Yang,Yuanchun Li,Mengjun Li,Yuelin Wang,Chenguang Shen,Songdong Meng,Liang Yang,Youjun Feng,Jiuxin Qu. 2023

[19]Isobavachalcone enhances sensitivity of colistin-resistant Klebsiella pneumoniae: In vitro and in vivo proof-of-concept studies. Xiang Geng,Yuxi Li,Ruochen Hao,Chunyan Xu,Zhun Li,Yajun Yang,Xiwang Liu,Jianyong Li,Wanxia Pu. 2024

[20]Naringin alleviates pneumonia caused by Klebsiella pneumoniae infection by suppressing NLRP3 inflammasome. Xiao Han Jing,Guan Yu Zhao,Gui Bo Wang,Qi Lin Huang,Wen Shu Zou,Li Na Huang,Wei Li,Zheng Ying Qiu,Rui Hua Xin. 2024

作者其他论文 更多>>