数字农科院2.0

Development of a Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Gallic Acid

文献类型： 外文期刊

作者： Jiajing Duan;Xiuxia Zheng;Ran Tao;Long Li;Fengzhong Wang;Yufeng Sun;Bei Fan

作者机构：

关键词： gallic acid;hapten;ic-ELISA;monoclonal antibody;rapid detection

期刊名称： Biosensors

ISSN： 2079-6374

年卷期： 2024 年 14 卷 4 期

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Gallic acid (GA) is closely related to the quality of herbal medicines and other agricultural products. In order to facilitate the rapid detection of GA, we developed a monoclonal antibody-based ic-ELISA method. Antigens with and without connecting arms were prepared. It was found that the introduction of connecting arms (linear carbon chain) was beneficial for immune response. By utilizing hybridoma technology, a specific mAb (anti-GA-M702) was screened and identified, which exhibited a 1:40,500 antibody titer and IgG2b antibody subtype. The ic-ELISA assay was established based on anti-GA-M702. The optimal working concentrations of the encapsulated antigen and antibody were 0.5 µg/mL and 0.67 µg/mL, respectively. The ic-ELISA method showed a linear detection range of 297.17–2426.61 ng/mL for GA with a sensitivity of 849.18 ng/mL. It displayed a good applicability for the determination of GA in Galla chinensis. In conclusion, the ic-ELISA method provides an efficient approach to the rapid detection of GA in products.

分类号：

  • 相关文献

[1]Production of a Monoclonal Antibody for the Detection of Forchlorfenuron: Application in an Indirect Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip. Xingmei Lei,A. M. Abd El-Aty,Lingyuan Xu,Jing Zhao,Jia Li,Song Gao,Yun Zhao,Yongxin She,Fen Jin,Jing Wang,Lufei Zheng,Maojun Jin,Bruce D. Hammock. 2023

[2]Generation of a highly specific recombinant full-length antibody for detecting ethirimol in fruit and environmental water. Xingmei Lei,Peipei Li,A. M. Abd El-Aty,Jing Zhao,Lingyuan Xu,Song Gao,Jia Li,Yun Zhao,Yongxin She,Fen Jin,Jing Wang,Lufei Zheng,Bruce D. Hammock,Maojun Jin. 2024

[3]Preparation of monoclonal antibodies recognizing pharmacologically active metabolites of metamizole based on rational hapten design and their application in the detection of animal-derived food. Linwei Zhang,Jiacan Wang,Yiting Wang,Hao Wen,Mingyue Ding,Jiaxu Xiao,Hongfei Yang,Xiaoming Pan,Shiyun Han,Dapeng Peng. 2024

[4]Preparation, identification, and preliminary application of monoclonal antibody against pyrethroid insecticide fenvalerate. Li, Peiwu,Zhang, Qi,Li, Peiwu,Zhang, Wen,Wang, Xiupin,Li, Peiwu.

[5]Development of a Monoclonal Antibody-Based Enzyme Immunoassay for the Pyrethroid Insecticide Deltamethrin. Kong, Ye,Zhang, Qi,Zhang, Wen,Li, Peiwu,Zhang, Wen,Li, Peiwu,Zhang, Qi,Gee, Shirley J..

[6]Obtaining a Monoclonal Antibody against a Novel Prometryn-Like Hapten and Characterization of Its Selectivity for Triazine Herbicides. Lingyuan Xu,A. M. Abd El-Aty,Jing Zhao,Xingmei Lei,Xiuyuan Zhang,Yun Zhao,Xueyan Cui,Yongxin She,Fen Jin,Jing Wang,Maojun Jin,Bruce D. Hammock. 2023

[7]Design and Characterization of a Novel Hapten and Preparation of Monoclonal Antibody for Detecting Atrazine. Lingyuan Xu,A. M.Abd El-Aty,Jae Han Shim,Jong Bang Eun,Xingmei Lei,Jing Zhao,Xiuyuan Zhang,Xueyan Cui,Yongxin She,Fen Jin,Lufei Zheng,Jing Wang,Maojun Jin,Bruce D. Hammock. 2022

[8]High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor. Yufeng Sun,Dong Zhu,Ran Tao,Long Li,Bei Fan,Fengzhong Wang. 2023

[9]Screening for Bongkrekic Acid in Food Using a Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay (icELISA). Wu, Hui-Ling,Chen, Jing,Pan, Fei,Xia, Qi-Sheng,Zhang, Shi-Wei,Chen, Xue-Jian,Feng, Rong-Hu,Zhang, Gui-Hong,Liu, Lei,Lai, Xin-Tian,Tan, Pan. 2024

[10]A Novel Immunochromatographic Strip Based on Latex Microspheres for the Rapid Detection of North American-Type Porcine Reproductive and Respiratory Syndrome Virus. Li, Wansheng,Li, Minhua,Zhang, Hongliang,Li, Chao,Xu, Hu,Gong, Bangjun,Fu, Jun,Guo, Zhenyang,Peng, Jinmei,Zhou, Guohui,Tian, Zhijun,Wang, Qian. 2022

[11]Preparation of specific nanobodies and their application in the rapid detection of nodularin-r in water samples. Jinyi Yang,Rui Si,Guangpei Wu,Yu Wang,Ruyu Fang,Fei Liu,Feng Wang,Hongtao Lei,Yudong Shen,Qi Zhang,Hong Wang. 2021

[12]Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips. Gao, Song,Abd El-Aty, A. M.,Xu, Lingyuan,Zhao, Jing,Li, Jia,Lei, Xingmei,Zhao, Yun,She, Yongxin,Jin, Fen,Wang, Jing,Yuan, Sichun,Jin, Maojun,Zheng, Lufei,Hammock, Bruce D.. 2024

[13]Effect of hapten structures on specific and sensitive enzyme-linked immunosorbent assays for N-methylcarbamate insecticide metolcarb. Zhang, Qi,Wu, Yunru,Wang, Limin,Hu, Baishi,Liu, Fengquan,Zhang, Qi,Li, Peiwu.

[14]Development of a sensitive ELISA for the analysis of the organophosphorous insecticide fenthion in fruit samples. Zhang, Qi,Sun, Qin,Hu, Baishi,Shen, Qing,Yang, Gang,Liang, Xiao,Sun, Xiao,Liu, Fengquan.

[15]Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples. Zhang, Qi,Zhang, Wen,Wang, Xiuping,Li, Peiwu. 2010

[16]Immunoassays for aflatoxins. Li, Peiwu,Zhang, Qi,Zhang, Wen.

[17]Generating Monoclonal Antibodies against Buprofezin and Developing Immunoassays for Its Residue Detection in Tea Samples. Lingyuan Xu,A. M. Abd El Aty,Zhen Cao,Xingmei Lei,Jing Zhao,Jia Li,Song Gao,Yun Zhao,Yongxin She,Fen Jin,Jing Wang,Maojun Jin,Bruce D. Hammock. 2023

[18]Unveil the molecular recognition mechanisms of triazine haptens and monoclonal antibodies: Establishing ic-ELISA methods for triazine herbicide analysis in tea. Xu, Lingyuan,Abd El-Aty, A. M.,Li, Peipei,Li, Jia,Zhao, Jing,Lei, Xingmei,Gao, Song,Zhao, Yun,She, Yongxin,Jin, Fen,Wang, Jing,Hammock, Bruce D.,Jin, Maojun. 2025

[19]Isocratic elution system for the determination of catechins, caffeine and gallic acid in green tea using HPLC. Wang, HF,Helliwell, K,You, XQ. 2000

[20]Effects of dietary gallic acid supplementation on performance, antioxidant status, and jejunum intestinal morphology in broiler chicks. Samuel, K. G.,Wang, J.,Yue, H. Y.,Wu, S. G.,Zhang, H. J.,Qi, G. H.,Duan, Z. Y..

作者其他论文 更多>>