数字农科院2.0

Biodegradation of azo dyes by a newly enriched extreme-thermophilic bacterial consortium

文献类型： 外文期刊

作者： Fang Tian;Guang Guo;Chong Liu;Jiuxiao Hao;Keqiang Ding;Feng Yang;Tingfeng Liu;Jin Xu;Zhengbing Guan;Soroush Abolfathi

作者机构：

关键词： azo dye;decolorization;extreme-thermophilic;metagenomic;textile wastewater

期刊名称： Journal of Water Process Engineering

ISSN： 2214-7144

年卷期： 2025 年 79 卷

页码：

收录情况： SCIE（2025版）

摘要： Textile wastewater of the temperature can reach to 50–80 °C, significantly inhibiting azo dye decolorization during conventional biological treatment. In this study, an extreme-thermophilic bacterial consortium CJ was enriched from textile wastewater, which can decolorize azo dye acid orange 7 (AO7) at 75 °C. The consortium CJ was dominated by Parageobacillus and Geobacillus. Its decolorization rate can reach 96 % within 36 h at initial dye concentration of 100 mg/L. Remarkably, the consortium retained high decolorization activity across a broad range of pH, salinity, temperature, and initial dye concentrations, demonstrating strong environmental adaptability. Enzymatic analysis revealed that azoreductase, Laccase (Lac), and manganese peroxidase (MnP) were key mediators of the decolorization process. AO7 degradation products were identified based on FTIR and GC–MS spectrometric detection, and metagenomic analysis identified several functional genes associated with azo dye degradation, including FMN-dependent NADH-azoreductase and FMN reductase. Based on metabolite profiling and genomic insights, a putative metabolic pathway for AO7 degradation was proposed. Furthermore, phytotoxicity assays indicated that the degradation products exhibited lower toxicity than the parent compound. This study provides critical insights into the mechanisms of azo dye biodegradation under extreme thermophilic conditions and highlights the potential application of consortium CJ for the treatment of high-temperature textile wastewater.

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