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High-throughput methods for genome editing: the more the better

文献类型： 外文期刊

作者： Huang, Yong;Shang, Meiqi;Liu, Tingting;Wang, Kejian

作者机构：

期刊名称： PLANT PHYSIOLOGY

ISSN： 0032-0889

年卷期： 2023 年 188 卷 4 期

页码：

收录情况： SCIE（2023版）

摘要： During the last decade, targeted genome-editing technologies, especially clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) technologies, have permitted efficient targeting of genomes, thereby modifying these genomes to offer tremendous opportunities for deciphering gene function and engineering beneficial traits in many biological systems. As a powerful genome-editing tool, the CRISPR/Cas systems, combined with the development of next-generation sequencing and many other high-throughput techniques, have thus been quickly developed into a high-throughput engineering strategy in animals and plants. Therefore, here, we review recent advances in using high-throughput genome-editing technologies in animals and plants, such as the high-throughput design of targeted guide RNA (gRNA), construction of large-scale pooled gRNA, and high-throughput genome-editing libraries, high-throughput detection of editing events, and high-throughput supervision of genome-editing products. Moreover, we outline perspectives for future applications, ranging from medication using gene therapy to crop improvement using high-throughput genome-editing technologies. The tremendous progress of high-throughput methods has brought genome editing into a high-throughput era, which provide great convenience for deciphering gene function and molecular design breeding.

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