数字农科院2.0

Fe-N-C single-atom nanozyme for ultrasensitive, on-site and multiplex detection of mycotoxins using lateral flow immunoassay

文献类型： 外文期刊

作者： Cai Xinfa; Ma Fei; Jiang Jun; Yang Xianglong; Zhang Zhaowei; Jian Zelang; Liang Meijuan; Li Peiwu; Yu Li

作者机构：

关键词： Atoms; Catalysis; Design for testability; Immunology; Iron compounds; Quantum chemistry; Catalytic mechanisms; Hydroxyl radicals; Lateral flow immunoassay; Multiple detection; Multiplex detections; On-site detection; Quantitative detection; Single-atom nanozyme; Single-atoms; Ultrasensitive; Mycotoxins; aflatoxin B1; fumonisin B1; hydroxyl radical; iron; mycotoxin; catalysis; catalyst; detection method; hydroxyl radical; immunoassay; nanoparticle; toxin; Article; catalysis; catalyst; comparative study; controlled study; density functional theory; electron spin resonance; European Union; evaluation study; extended X ray absorption fine structure spectroscopy; high temperature; immunoassay; lateral flow immunochromatography; limit of detection; morphology; photon correlation spectroscopy; practice guideline; quantitative analysis; simulation; synthesis; theoretical study; transmission electron microscopy; X ray photoemission spectroscopy

期刊名称： Journal of Hazardous Materials

ISSN： 0304-3894

年卷期： 2022 年 441 卷

页码：

收录情况： SCIE（2022版） ; EI（2022版）

摘要： Sensitive, on-site and multiple detection of mycotoxins is a vital early-warning tool to minimize food losses and protect human health and the environment. Although paper-based lateral flow immunoassay (LFIA) has been extensively applied in mycotoxins monitoring, low-cost, portable, ultrasensitive and quantitative detection is still a formidable challenge. Herein, a series of Fe-N-C single-atom nanozymes (SAzymes) were synthesized and systematic characterized. The optimal Fe-N-C SAzyme with highly efficient catalytic performance was successfully used as both label and catalyst in lateral flow immunoassays for mycotoxin detection. By taking advantage of the catalytic amplified system, the qualitative and quantitative detection can be easily and flexibly done via observing the test lines by naked eyes or a smartphone, with the limit of detections (LODs) of 2.8 and 13.9 pg mL−1 for AFB1 and FB1, which were respectively over 700- and 71,000-fold lower than the maximum limit set by the European Union. Besides, underlying catalytic mechanisms and the active sites of the Fe-N-C SAzyme are also investigated by DFT simulation. This work not only provides a promising detection strategy for the application of advanced SAzymes but also offers experimental and theoretical guidelines to understand the active centers of Fe-N-C SAzymes and the catalytic process. © 2022 Elsevier B.V.

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