数字农科院2.0

Transcription factors PgWRKY21 and PgWRKY101 regulate protopanaxatriol ginsenoside biosynthesis in Panax ginseng

文献类型： 外文期刊

作者： Ping Wang;Yihan Wang;Junmei Lian;Yan Yan;Xiao Xing;Hao Zhang;Xiangmin Piao;Yonghua Xu;Yan Sui;Peng Di;Limin Yang;Yingping Wang

作者机构：

关键词： Ginsenosides;Panax ginseng;Regulation;Transcriptomic;WRKY transcription factor

期刊名称： Industrial Crops and Products

ISSN： 0926-6690

年卷期： 2025 年 236 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Ginsenosides, the primary secondary metabolites derived from Panax ginseng, are in high demand. Cultured tissues provide a promising approach to enhance ginsenoside production and meet this demand. This study investigated ginsenoside accumulation and gene expression profiles across various P. ginseng tissues, including callus, adventitious roots, and fibrous roots. Among the cultured tissues analyzed, callus exhibited significantly higher contents of ginsenosides Ro, 20(R)-Rg2, and Rg1. Additionally, key biosynthetic genes, including PgPPTS1, PgSQE10, and PgDDS2, showed elevated expression in callus tissues. Differential expression analyses identified numerous transcription factors (TFs) from the bHLH, MYB, WRKY, and NAC families, with multiple WRKY TFs significantly associated with ginsenoside biosynthesis. Correlation analysis revealed strong positive relationships between the expression of WRKY TFs (PgWRKY21, PgWRKY101, and PgWRKY135) and protopanaxatriol synthase (PPTS). Subcellular localization confirmed nuclear localization of these TFs. Yeast one-hybrid assays demonstrated that PgWRKY21 and PgWRKY101 directly bind to W-box elements in the promoter region. Dual-luciferase assays indicated that PgWRKY21 and PgWRKY101 significantly enhanced PgPPTS1 promoter-driven expression. Overexpression of PgWRKY21 and PgWRKY101 in transgenic P. ginseng callus resulted in an 8.79-fold and 6.83-fold increase in PgPPTS1 transcript abundance, respectively. Correspondingly, these lines exhibited significant increases in ginsenoside content, PgWRKY21-overexpressing lines showed 2.48-, 1.89-, and 1.57-fold increases in Re, Rf, and Rg1, respectively, whereas PgWRKY101-overexpressing lines demonstrated 2.17-, 2.33-, and 1.43-fold increases. These findings highlight the crucial roles of PgWRKY21 and PgWRKY101 in regulating ginsenoside production and suggest their potential as targets for metabolic engineering strategies in P. ginseng.

分类号：

  • 相关文献

[1]High-Performance Liquid Chromatography with Diode Array Detector and Electrospray Ionization Ion Trap Time-of-Flight Tandem Mass Spectrometry to Evaluate Ginseng Roots and Rhizomes from Different Regions. Wang, Hong-Ping,Zhang, You-Bo,Yang, Xiu-Wei,Yang, Xin-Bao,Xu, Wei,Xu, Feng,Cai, Shao-Qing,Wang, Ying-Ping,Xu, Yong-Hua,Zhang, Lian-Xue.

[2]Molecular Docking and Dynamics Simulation Studies of Ginsenosides with SARS-CoV-2 Host and Viral Entry Protein Targets. Pang Shifeng,Vinothini Boopathi,Mohanapriya Murugesan,Ramya Mathiyalagan,Jong Chan Ahn,Chen Xiaolin,Dong Uk Yang,Gi Young Kwak,Byoung Man Kong,Deok Chun Yang,Se Chan Kang,Zhang Hao. 2022

[3]Identification of anti-inflammatory components in Panax ginseng of Sijunzi Decoction based on spectrum-effect relationship. Kan, Hong,Zhang, Dongxue,Chen, Weijia,Wang, Shihan,He, Zhongmei,Pang, Shifeng,Qu, Shuai,Wang, Yingping. 2023

[4]Progress on the efficacy and mechanism of action of panax ginseng monomer saponins treat toxicity. Xinyi Wang,Rongcan Wang,Yongfei Qiao,Yali Li. 2022

[5]Effect of Phosphate-Deficiency Stress on the Biological Characteristics and Transcriptomics of Panax ginseng. Hai Sun,Hao Liang,Cai Shao,Jiaqi Qian,Jiapeng Zhu,Guojia Zhang,Bochen Lv,Yayu Zhang. 2024

[6]Intercropping Between Panax ginseng and Arisaema amurense Improves Ginseng Quality by Improving Soil Properties and Microbial Communities. Bochen Lv,Hai Sun,Weiyu Cao,Jiapeng Zhu,Hao Liang,Hongjie Long,Yanmei Cui,Cai Shao,Yayu Zhang. 2025

[7]Ginsenoside, a potential natural product against liver diseases: a comprehensive review from molecular mechanisms to application. Shang, Shiyan,Yang, Haixia,Qu, Linlin,Fan, Daidi,Deng, Jianjun. 2025

[8]Structure-activity relationship of ginsenoside derivatives with different glycosides and double bond position on anti-aging bioactivities. Zhang, Juntao,Wang, Weimin,Fan, Daidi,Deng, Jianjun,Yang, Haixia. 2025

[9]Comparison of different ginsenosides with C-3 or C-6 sugar moieties on activities in alcohol-induced liver injury mice. Wang, Weimin,Zhou, Kaixuan,Fu, Zengshuai,Huang, Yucheng,Deng, Jianjun,Fan, Daidi,Yang, Haixia. 2025

[10]Profiling the Differential Distribution of Ginsenosides Across Ginseng Tissues Using High-Resolution Mass Spectrometry. Hang Xu,Zheng Li,Chong Liu,Yukun Wang,Siwei Qiao,Hao Zhang. 2025

[11]Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean. Wu, Jing,Wang, Lanfen,Wang, Shumin,Chen, Jibao. 2016

[12]Genome-wide analysis of the WRKY gene family in cotton. Dou, Lingling,Zhang, Xiaohong,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Yu, Shuxun.

[13]Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis. 何冠华,徐兆师. 2016

[14]Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis. Yimian Ma,Jiadong Ran,Guoqiong Li,Mengchen Wang,Chengmin Yang,Xin Wen,Xin Geng,Liping Zhang,Yuan Li,Zheng Zhang. 2023

[15]A VQ-motif-containing protein fine-tunes rice immunity and growth by a hierarchical regulatory mechanism. Hao Z.,Tian J.,Fang H.,Fang L.,Xu X.,He F.,Li S.,Xie W.,Du Q.,You X.,Wang D.,Chen Q.,Wang R.,Zuo S.,Yuan M.,Wang G.-L.,Xia L.,Ning Y.. 2022

[16]Setaria italica SiWRKY89 enhances drought tolerance in Arabidopsis. Zhang, Aiying,Zhang, Li,Guo, Erhu,Wang, Rui,Li, Qiang,Guo, Shihua,Marowa, Prince,Xu, Zongchang. 2022

[17]TaGSNE, a WRKY transcription factor, overcomes the trade-off between grain size and grain number in common wheat and is associated with root development. Khan, Nadia,Zhang, Yanfei,Wang, Jingyi,Li, Yuying,Chen, Xin,Yang, Lili,Zhang, Jie,Li, Chaonan,Li, Long,Rehman, Shoaib Ur,Reynolds, Matthew P.,Zhang, Lichao,Zhang, Xueyong,Mao, Xinguo,Jing, Ruilian. 2022

[18]Genome-Wide Characterization of the Maize (Zea mays L.) WRKY Transcription Factor Family and Their Responses to Ustilago maydis. Yang Wang,Wangshu Li,Jianzhou Qu,Fenghai Li,Wanli Du,Jianfeng Weng. 2023

[19]MsWRKY33 increases alfalfa (Medicago sativa L.) salt stress tolerance through altering the ROS scavenger via activating MsERF5 transcription. Ma, Lin,Li, Xin,Zhang, Jinjin,Yi, Dengxia,Li, Feng,Wen, Hongyu,Liu, Wenhui,Wang, Xuemin. 2023

[20]CsWRKY12 interacts with CsVQ4L to promote the accumulation of galloylated catechins in tender leaves of tea plants. Zhang, Yongheng,Wang, Jie,Xiao, Yezi,Wu, Yedie,Li, Nana,Ding, Changqing,Hao, Xinyuan,Yu, Youben,Wang, Lu,Wang, Xinchao. 2024

作者其他论文 更多>>