数字农科院2.0

High-Yield Preparation of American Oyster Defensin (AOD) via a Small and Acidic Fusion Tag and Its Functional Characterization

文献类型： 外文期刊

作者： Zhao Q.;Yang N.;Gu X.;Li Y.;Teng D.;Hao Y.;Lu H.;Mao R.;Wang J.

作者机构：

关键词： American Oyster Defensin (AOD);antimicrobial mechanism;antimicrobial peptides;pharmacodynamics;small fusion tag

期刊名称： Marine Drugs

ISSN： 1660-3397

年卷期： 2024 年 22 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： The marine peptide, American oyster defensin (AOD), is derived from Crassostrea virginica and exhibits a potent bactericidal effect. However, recombinant preparation has not been achieved due to the high charge and hydrophobicity. Although the traditional fusion tags such as Trx and SUMO shield the effects of target peptides on the host, their large molecular weight (12–20 kDa) leads to the yields lower than 20% of the fusion protein. In this study, a short and acidic fusion tag was employed with a compact structure of only 1 kDa. Following 72 h of induction in a 5 L fermenter, the supernatant exhibited a total protein concentration of 587 mg/L. The recombinant AOD was subsequently purified through affinity chromatography and enterokinase cleavage, resulting in the final yield of 216 mg/L and a purity exceeding 93%. The minimum inhibitory concentrations (MICs) of AOD against Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus galactis ranged from 4 to 8 μg/mL. Moreover, time-killing curves indicated that AOD achieved a bactericidal rate of 99.9% against the clinical strain S. epidermidis G-81 within 0.5 h at concentrations of 2× and 4× MIC. Additionally, the activity of AOD was unchanged after treatment with artificial gastric fluid and intestinal fluid for 4 h. Biocompatibility testing demonstrated that AOD, at a concentration of 128 μg/mL, exhibited a hemolysis rate of less than 0.5% and a cell survival rate of over 83%. Furthermore, AOD’s in vivo therapeutic efficacy against mouse subcutaneous abscess revealed its capability to restrain bacterial proliferation and reduce bacterial load, surpassing that of antibiotic lincomycin. These findings indicate AOD’s potential for clinical usage. © 2023 by the authors.

分类号：

  • 相关文献

[1]The Marine Antimicrobial Peptide AOD with Intact Disulfide Bonds Has Remarkable Antibacterial and Anti-Biofilm Activity. Ruoyu Mao,Qingyi Zhao,Haiqiang Lu,Na Yang,Yuanyuan Li,Da Teng,Ya Hao,Xinxi Gu,Jianhua Wang. 2024

[2]In Vitro Pharmacodynamics and Bactericidal Mechanism of Fungal Defensin-Derived Peptides NZX and P2 against Streptococcus agalactiae. Yankang Wu,Na Yang,Ruoyu Mao,Ya Hao,Da Teng,Jianhua Wang. 2022

[3]Expression and characterization of recombinant gallinacin-9 and gallinacin-8 in Escherichia coli. Ma, De-ying,Shan, An-shan,Liu, Sheng-wang,Han, Zong-xi,Li, Yi-jin.

[4]Design and pharmacodynamics of recombinant fungus defensin nzl with improved activity against staphylococcus hyicus in vitro and in vivo. Liu He,Yang Na,Teng Da,Mao Ruoyu,Hao Ya,Ma Xuanxuan,Wang Jianhua. 2021

[5]Marine Antimicrobial Peptides: Advances in Discovery, Multifunctional Mechanisms, and Therapeutic Translation Challenges. Bin Gao,Na Yang,Da Teng,Ya Hao,Jianhua Wang,Ruoyu Mao. 2025

[6]Lactic Acid Bacteria as the Green and Safe Food Preservatives: Their Mechanisms, Applications and Prospects. Zhang, Yuwei,Li, Lianrui,Pang, Xiaoyang,Zhang, Shuwen,Liu, Yang,Wang, Yunna,Xie, Ning,Li, Xu. 2026

[7]Schistosoma japonicum: Treatment of different developmental stages in mice with long-acting praziquantel implants. Cheng, Liang,Lei, Lei,Guo, Shengrong,Rong, Haojun,Guo, Dongwei,Zhu, Chuangang,Zhang, Lei,Jiang, Yan,Lin, Jiaojiao.

[8]Plasmid containing CpG oligodeoxynucleotides can augment the immune responses of pigs immunized with porcine reproductive and respiratory syndrome killed virus vaccine. Quan, Zhang,Qin, Zhai Guo,Zhen, Wang,Feng, Xue Zheng,Hong, Jia,Fei, Zhu Hong.

[9]Reassortant H1N1 influenza virus vaccines protect pigs against pandemic H1N1 influenza virus and H1N2 swine influenza virus challenge. Yang, Huanliang,Chen, Yan,Shi, Jianzhong,Guo, Jing,Xin, Xiaoguang,Zhang, Jian,Qiao, Chuanling,Chen, Hualan,Wang, Dayan,Shu, Yuelong.

[10]FMD subunit vaccine produced using a silkworm-baculovirus expression system: Protective efficacy against two type Asia1 isolates in cattle. Li, Zhiyong,Yin, Xiangping,Li, Xuerui,Li, Baoyu,Lan, Xi,Liu, Jixing,Yi, Yongzhu,Zhang, Zhifang.

[11]Pharmacokinetics and Pharmacodynamics of Fungal Defensin NZX Against Staphylococcus aureus-Induced Mouse Peritonitis Model. Zheng, Xueling,Yang, Na,Mao, Ruoyu,Hao, Ya,Teng, Da,Wang, Jianhua. 2022

[12]Pharmacokinetics and pharmacodynamics of antibacterial peptide NZX in Staphylococcus aureus mastitis mouse model. Xueling Zheng,Na Yang,Ruoyu Mao,Ya Hao,Da Teng,Jianhua Wang. 2024

[13]Antimicrobial Peptides From Different Plant S.ources: Isolation, Characterisation, And P urification. Tang, Swee-Seong,Tang, Swee-Seong,Sekaran, Shamala D.,Prodhan, Zakaria H.,Le, Cheng-Foh,Biswas, Sudhangshu K.,Prodhan, Zakaria H.. 2018

[14]EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus. Ke, Tao,Cao, Huihui,Huang, Junyan,Dong, Caihua,Yu, Jingyin,Mao, Han,Liu, Shengyi,Ke, Tao,Wang, Xi,Niu, Qiuhong,Hui, Fengli,Huang, Junyan,Hu, Fan,Huang, Jin,Dong, Caihua,Ma, Xiangdong,Liu, Shengyi. 2015

[15]Design and characterization of novel hybrid peptides from LFB15(W4,10), HP(2-20), and cecropin A based on structure parameters by computer-aided method. Tian, Zi-gang,Dong, Tian-tang,Teng, Da,Yang, Ya-lin,Wang, Jian-hua.

[16]Mechanism of action of a novel recombinant peptide, MP1102, against Clostridium perfringens type C. Zong, Lifen,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Yang, Na,Hao, Ya,Wang, Jianhua,Zong, Lifen,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Yang, Na,Hao, Ya,Wang, Jianhua.

[17]Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum. Jian Gui-Liang,Zhang Ying-Tao,Ai Tie-Min.

[18]Fusion expression of bovine lactoferricin in Escherichia coli. Feng, Xing-jun,Wang, Jian-hua,Shan, An-shan,Teng, Da,Yang, Ya-lin,Yao, Yi,Yang, Guan-pin,Shao, Yan-chun,Liu, Shuo,Zhang, Fan.

[19]Gene Expression Profiling of Cecropin B-Resistant Haemophilus parasuis. Wang, Chunmei,Chen, Fangzhou,Hu, Han,Li, Wentao,Chen, Pin,Liu, Yingyu,Ku, Xugang,He, Qigai,Chen, Huanchun,Wang, Chunmei,Xue, Feiqun,Wang, Yang.

[20]In vitro and in vivo characterization of a new recombinant antimicrobial peptide, MP1102, against methicillin-resistant Staphylococcus aureus. Zhang, Yong,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Cao, Xintao,Hu, Xiaoyuan,Zong, Lifen,Wang, Jianhua,Zhang, Yong,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Cao, Xintao,Hu, Xiaoyuan,Zong, Lifen,Wang, Jianhua.

作者其他论文 更多>>