A salt/salt aqueous two-phase system based on pH-switchable deep eutectic solvent for the extraction and separation of mulberry polysaccharides
文献类型: 外文期刊
作者: Lei, Tian;Qin, Zongkui;Liu, Leilei;Tan, Zhijian
作者机构:
关键词: Switchable deep eutectic solvents;Aqueous two-phase systems;Separation and purification;Mulberry;Polysaccharides
期刊名称: FOOD CHEMISTRY
ISSN: 0308-8146
年卷期: 2024 年 462 卷
页码:
收录情况: SCIE(2024版) ; ; EI(2024版)
摘要: With the aim of expanding the potential application scope of mulberries, eleven pH-switchable deep eutectic solvents were screened for the ultrasonic-assisted extraction of mulberry polysaccharides, and a salt/salt aqueous two-phase system was constructed for the efficient separation of mulberry polysaccharides by regulating the system pH. DES-9 (tetraethylammonium chloride: octanoic acid molar ratio = 1: 2) with a critical response pH value of approximately 6.1 was concluded to be the best extraction solvent for extracting mulberry polysaccharides. A maximum polysaccharide extraction yield of 270.71 mg/g was obtained under the optimal conditions. The maximum polysaccharide extraction efficiency was 78.09 % for the pH-driven tetraethylammonium chloride/K2HPO4 aqueous two-phase system. An acidic beta-pyran mulberry polysaccharide with a low-molecular weight of 9.26 kDa and a confirmed monosaccharide composition were obtained. This efficient and environmentally friendly polysaccharide separation method offers a new approach for the efficient extraction and utilization of other plant polysaccharides.
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