数字农科院2.0

Digital PCR-Based Characterization of a g10evo-epsps Gene-Specific Matrix Reference Material for Its Food and Feed Detection

文献类型： 外文期刊

作者： Xiaoyun Chen;Huiru Yu;Pengfei Wang;Cheng Peng;Xiaofu Wang;Xiaoli Xu;Junfeng Xu;Jingang Liang;Liang Li

作者机构：

关键词： certified reference material;digital PCR;g10evo-epsps;glyphosate resistance;ZUTS-33 soybean

期刊名称： Foods

ISSN： 2304-8158

年卷期： 2022 年 11.0 卷 13 期

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摘要： g10evo-epsps is a novel glyphosate herbicide-resistant gene that has been transferred to various crops such as soybean, corn, cotton, and rice. Here, we developed a gene-specific digital Polymerase Chain Reaction (dPCR) detection method for absolute quantitative analysis of g10evo-epsps, and characterized g10evo-epsps certified reference materials (CRM) using ZUTS-33 soybean powder as the candidate material. Stability tests of matrix CRMs demonstrate that these CRMs can be stored stably for 6 months and transported for 10 days at room temperature and withstand summer high temperatures (below 60 °C). CRM characterization is based on the copy number ratio of g10evo-epsps to lectin. Eight qualified laboratories independently validated the CRM using dPCR method, with a measurement of 0.98 (copy/copy) and an extended uncertainty of 0.08 (copy/copy). The g10evo-epsps matrix CRM described here may be used for qualitative and quantitative testing, method evaluation, laboratory quality control, and other related fields.

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