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Transcriptomic Insights Into Watermelon (Citrullus lanatus) Resistance to Fusarium oxysporum f. sp. niveum: Hormonal Cross-Talk and Defence-Related Gene Regulation

文献类型: 外文期刊

作者: Zheng, Yang;Bai, Ziqin;Mu, Rong;Gu, Qinsheng;Jiang, Jihong;Sun, Xianchao;Chen, Guokang;Huang, Kuo;Ren, Jiequn;Ru, Xuejuan

作者机构:

关键词: Fusarium oxysporum f. sp. niveum;Fusarium wilt;transcriptome;watermelon

期刊名称: JOURNAL OF PHYTOPATHOLOGY

ISSN: 0931-1785

年卷期: 2025 年 173 卷 5 期

页码:

收录情况: SCIE(2025版)

摘要: Fusarium wilt, caused by Fusarium oxysporum f. sp. niveum (FON), is a major threat to watermelon (Citrullus lanatus) production worldwide. In this study, we characterised the biological properties of a highly virulent FON isolate and evaluated the resistance of 10 watermelon genotypes. Optimal growth of the pathogen occurred at 25 degrees C and pH 7.0, and resistance levels among watermelon materials showed clear differentiation. Transcriptome analysis of a resistant (WX01) and a susceptible (WX07) genotype after FON inoculation revealed 2640 differentially expressed genes (DEGs). Functional enrichment indicated that DEGs were mainly involved in plant-pathogen interaction, hormone signal transduction, and phenylpropanoid biosynthesis. Notably, key genes associated with jasmonic acid (e.g., JAZ), salicylic acid (e.g., TGA), and auxin (e.g., GH3) signalling pathways exhibited significant expression differences between the two genotypes. qRT-PCR validation confirmed the transcriptomic results. Downregulation of JAZ and upregulation of TGA, GH3, and phenylpropanoid-related genes in WX01 suggest a coordinated defence response involving hormonal cross-talk and secondary metabolism. These findings provide new insights into the molecular basis of Fusarium wilt resistance in watermelon and identify candidate genes for breeding disease-resistant cultivars.

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