数字农科院2.0

An efficient and low-cost method for the determination of selenium in selenium-enriched tea by high-performance liquid chromatography coupled with 3,3′-Diaminobenzidine derivatization

文献类型： 外文期刊

作者： He, Xiwen;Huangfu, Kai;Zhao, Juan;Lei, Hao;Li, Xiang;Liu, Wuyan;Gu, Xu

作者机构：

关键词： Selenium;Selenium-enriched tea;3,3 ' -diaminobenzidine;Derivative reaction;High-performance liquid chromatography

期刊名称： TALANTA

ISSN： 0039-9140

年卷期： 2024 年 268 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： In this study, a novel analytical method was developed and validated for the quantification of selenium in selenium-enriched tea. The various valence states of selenium present in tea were converted to Se(IV) through oxidation and reduction reactions. To chemically react with Se(IV), 3,3 '-Diaminobenzidine (DAB) was employed to produce Se-DAB derivatives. These derivatives were then analyzed and quantified using high-performance liquid chromatography (HPLC) with a NH2 column specifically designed for the target compound. The peak area of Se-DAB exhibited excellent linearity within the concentration range of 0.01-50 mu gmL(-1) (R-2 = 0.9999). The method demonstrated a limit of detection (LOD) of 0.01 mgkg(-1) and a limit of quantification (LOQ) of 0.03 mgkg(-1). Moreover, the recoveries of Se-DAB spiked at different concentrations ranged from 81.2 % to 96.7 %, with relative deviations (RSDs) of 0.72 %-4.23 % (n = 6). Additionally, the RSDs under different batches varied from 3.42 % to 8.57 % (n = 9). The method exhibits good stability and repeatability. The results obtained by applying the method to 24 samples of selenium-rich tea show high consistency with the AFS method. The difference range between the two methods is 0.017-0.027 mg/kg, with an average difference of 0.005 mg/kg. Furthermore, this method offers the advantage of flexibility in analyzing large quantities of green tea samples, while utilizing lower instrument and reagent costs. Therefore, it can be considered as a new and cost-effective method for detecting selenium content in selenium-rich tea.

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