数字农科院2.0

Engineering high-fertility synthetic apomixis in hybrid rice via genome editing of MiMe and OspPLAIIκ

文献类型： 外文期刊

作者： Chaolei Liu#; Yuye Chen#; Tingting Sun#; Jian Wang; Huan Liang; Keming Zhu*; Qian Qian*; Kejian Wang*

关键词： high-fertility synthetic apomixis genome editing

期刊名称： Science China-Life Sciences

ISSN： 1674-7305

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： our study shows that knockout of OspPLA/lx, a member of the PLA superfamily,induces haploid formation in rice, adding a new genetic resource for haploid induction alongsidepreviously reported genes such as OsMTL, OsMATL2, and OsPLDa2 (Wang et al., 2019; Jang et al., 2023; Hu et al., 2025). More importantly, combining OspPLAllk mutation with the MiMe strategy enabled the establishment of a synthetic apomixis system that offers two key advantages over existing systems. First, the Fix7 line displayed substantially improved fertility, with seed setting rates of 74.2-77.5%, in contrast to only 3.7-5.2% in the earlier OsMTL-based Fix system (Wang et al., 2019), thereby overcoming a major constraint in previous genome-edited apomictic systems. Second, unlike systems dependent on ectopic expression of parthenogenesis-related genes, the Fix7 system was constructed entirely through genome editing, avoiding persistent foreign DNA and providing a more regulatorily viable alternative. To further improve the clonal seed rate, OspPLAIIκ could be combined with other haploid induction genes such as OsPLDα2 (Hu et al., 2025). Moreover, the use of ribonucleoprotein (RNP)-mediated CRISPR/Cas9 technology could eliminate exogenous elements such as Cas9 and selectable markers, ultimately enabling a completely transgene-free synthetic apomixis system.

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