数字农科院2.0

Review on microbial recombination expression of antimicrobial peptides

文献类型： 外文期刊

作者： Xuan Li;Ya Hao;Da Teng;Ruoyu Mao;Na Yang;Jianhua Wang

作者机构：

关键词： Antimicrobial peptides;Expert opinion;High yield and low cost;Microbial hetero-expression systems;R&D pipeline and scaffold

期刊名称： Chemical Engineering Journal

ISSN： 1385-8947

年卷期： 2025 年 525 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Antimicrobial peptides (AMPs) exist extensively in all living organisms and play key roles in innate defense responses infection. AMPs have attracted much attention for years owing to their richer sources and unique merits compared to traditional antibiotics, as possible alternatives to antibiotics (ATAs) to buffer their overloading or abused. Recombinant microbial expression of exogenous proteins is a mature technology, but there are still limitations, such as low yield, high cost, toxicity, and loss of bioactivity, especially for AMPs. Digesting and integrating the merits of the four typical microbial recombination systems of AMPs would help to unblock the bottleneck of suicide expression, subdivide pathways and protocols in details, validate technical scaffolds of recombination procedures, patch up the short-slab of post-transformational modification and weak-stabilization, merge the gap of the various AMP R&D pipeline-networks, and finally lay a foundation for the druggability basis as a package solution and tool box focusing on digging and developing new types of AMP candidate. Finally, a constructive expert opinion about the future R&D direction and roadmap of AMP as a new drugs is presented for the purpose of unchoking R&D pipeline of AMP with high yield and low cost via heterologous expression.

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