数字农科院2.0

Engineering resistance genes against tomato brown rugose fruit virus

文献类型： 外文期刊

作者： Wang, Junzhu;Shen, Tianxin;Song, Mengjie;Fan, Jiayi;Li, Yiqing;Chin, Toto Zen;Yu, Yidan;Huang, Fan;Yang, Xiuling;Li, Changbao;Zhou, Xueping;Li, Chuanyou;Hong, Yiguo;Liu, Yule

作者机构：

关键词： Tomato brown rugose fruit virus;Tm-2(2);mutagenesis;resistance;movement protein

期刊名称： SCIENCE CHINA-LIFE SCIENCES

ISSN： 1674-7305

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Tomato brown rugose fruit virus (ToBRFV) overcomes all known tomato resistance genes, including the durable Tm-2(2), posing a serious threat to global tomato production. Here, we employed in vitro random mutagenesis to evolve the Tm-2(2) leucine-rich repeat (LRR) domain and screened similar to 8,000 variants for gain-of-function mutants capable of recognizing the ToBRFV movement protein (MP) and triggering hypersensitive cell death. We identified five such mutants. Among them, Tm-2(2-S723Y) and Tm-2(2-N744D) induced strong, specific cell death upon co-expression with ToBRFV MP, while retaining recognition of TMV MP. Transgenic Nicotiana benthamiana and tomato plants expressing Tm-2(2-S723Y) resisted the naturally occurring ToBRFV (ToBRFV(E132)). However, after prolonged infection, ToBRFV(E132) evolved an E132K substitution in its MP, and the newly appeared ToBRFV(K132) evaded Tm-2(2-S723Y)-mediated resistance. However, Tm-2(2-N744D) still recognized MPK132, and the double mutant Tm-2(2-S723Y/N744D) conferred broad resistance against ToBRFV(E132), ToBRFV(K132), and tobacco mosaic virus in stable transgenic tomato. Our work demonstrates that artificial NLR evolution can generate broad-spectrum resistance against tobamovirus.

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