数字农科院2.0

Comprehensive Characterization of Metabolites in Multiplier Onion Bulbs and Identification of Regulatory Genes for Nutritional Improvement

文献类型： 外文期刊

作者： Jia, Huixia;Song, Jiangping;Zhang, Tingting;Tan, Yumin;Wang, Mengzhen;Zang, Jiyan;Zhang, Xiaohui;Yang, Wenlong;Pang, Yanhui;Yang, Yanfei;Wang, Haiping

作者机构：

关键词： multiplier onion;metabolome;transcriptome;nutritional composition;regulatory genes

期刊名称： FOODS

ISSN：

年卷期： 2025 年 14 卷 19 期

页码：

收录情况： SCIE（2025版）

摘要： Multiplier onion (Allium cepa var. aggregatum) is an economically important Allium crop that serves dual purposes as both a culinary ingredient and medicinal resource. Despite its widespread utilization, systematic characterization of its nutrient metabolism components remains limited, which has constrained the development of high-value cultivars with optimized nutritional profiles. In this study, we conducted a comprehensive metabolomic profiling of bulbs from five genetically distinct accessions using a widely targeted metabolomic method based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The analysis identified 659 metabolites, including lipids, flavonoids, phenolic acids, amino acids, saccharides and alcohols, organic acids, alkaloids, nucleotides and derivatives, vitamins, etc. Notably, the bulbs exhibited a high abundance of flavonoids (e.g., quercetin, kaempferol, naringenin, isorhamnetin) and eight essential amino acids (valine, threonine, leucine, isoleucine, lysine, methionine, histidine, and tryptophan). Comparative analysis revealed that 366 differentially accumulated metabolites were identified among these 5 accessions, many of which were significantly enriched in pathways related to flavonoid biosynthesis, as well as amino acid biosynthesis and metabolism. Transcriptome analysis indicated that differentially expressed genes (DEGs) across the five accessions were significantly enriched flavonoid biosynthesis, and various amino acid biosynthesis and metabolism processes, such as tyrosine metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis, cysteine and methionine metabolism and arginine and proline metabolism, being consistent with the substantial variations observed in flavonoids, amino acids, and their derivatives in the metabolome analysis. Correlation and network analysis identified several crucial candidate regulatory genes for the biosynthesis and metabolism of lipids, flavonoids, phenolic acids, and amino acids. These findings provide a comprehensive metabolic atlas of multiplier onion, reveal key genetic regulators of nutritional quality, and establish a scientific foundation for nutraceutical development and cultivar improvement strategies.

分类号：

  • 相关文献

[1]Comprehensive Analysis of Full-Length Transcriptome Profiling, Genetic and Phenotypic Variation in Multiplier Onion (Allium cepa var. aggregatum) Accessions in China. Huixia Jia,Jiangping Song,Yuru Huang,Tingting Zhang,Mengzhen Wang,Yumin Tan,Jiyan Zang,Xiaohui Zhang,Wenlong Yang,Yanhui Pang,Yanfei Yang,Haiping Wang. 2025

[2]Multi-omics integration to explore the molecular insight into the volatile organic compounds in watermelon. Chengsheng Gong,Nan He,Hongju Zhu,Muhammad Anees,Xuqiang Lu,Wenge Liu. 2023

[3]Defensive Resistance of Cowpea Vigna unguiculata Control Megalurothrips usitatus Mediated by Jasmonic Acid or Insect Damage. Tao Li,Mingyue Feng,Yuanming Chi,Xing Shi,Zilin Sun,Zhen Wu,Aomei Li,Wangpeng Shi. 2023

[4]Transcriptomic and metabolomic analyses reveal that exogenous strigolactones alleviate the response of melon root to cadmium stress. Chen X.,Shi X.,Ai Q.,Han J.,Wang H.,Fu Q.. 2022

[5]Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton. Zhenzhen Wang,Xiaomeng Zhang,Shoupu He,Abdul Rehman,Yinhua Jia,Hongge Li,Zhaoe Pan,Xiaoli Geng,Qiong Gao,Liru Wang,Zhen Peng,Xiongming Du. 2022

[6]Transcriptomic and Metabolomic Analysis of Wheat Kernels in Response to the Feeding of Orange Wheat Blossom Midges (Sitodiplosis mosellana) in the Field. Qian Wang,Xiaobei Liu,Huan Liu,Yu Fu,Yumeng Cheng,Lijiao Zhang,Wangpeng Shi,Yong Zhang,Julian Chen. 2022

[7]Integrated Transcriptome and Metabolome Dissecting Interaction between Vitis vinifera L. and Grapevine Fabavirus. Baodong Zhang,Mengyan Zhang,Xiaojun Jia,Guojun Hu,Fang Ren,Xudong Fan,Yafeng Dong. 2023

[8]Integrated Metabolome and Transcriptome Analysis Unveils the Underlying Molecular Response of Panax ginseng Plants to the Phytophthora cactorum Infection. Hong Kan,Shuai Qu,Kai Dong,Shihan Wang,Chen Xu,Yingping Wang,Shuang Hua. 2023

[9]Integrated metabolomics and transcriptomics insights on flavonoid biosynthesis of a medicinal functional forage, Agriophyllum squarrosum (L.), based on a common garden trial covering six ecotypes. Tingzhou Fang,Shanshan Zhou,Chaoju Qian,Xia Yan,Xiaoyue Yin,Xingke Fan,Pengshu Zhao,Yuqiu Liao,Liang Shi,Yuxiao Chang,Xiao Fei Ma. 2022

[10]Multi-omics analyses of 398 foxtail millet accessions reveal genomic regions associated with domestication, metabolite traits, and anti-inflammatory effects. Xukai Li,Jianhua Gao,Jingyi Song,Kai Guo,Siyu Hou,Xingchun Wang,Qiang He,Yanyan Zhang,Yakun Zhang,Yulu Yang,Jiaoyan Tang,Hailang Wang,Staffan Persson,Mingquan Huang,Lishuai Xu,Linlin Zhong,Dongqin Li,Yongming Liu,Hua Wu,Xianmin Diao,Pe. 2022

[11]Integrative analysis of metabolome and genome-wide transcriptome reveal the flavor changes in apple (Malus pumila Mill) after the novel acaricide cyflumetofen application. Minmin Li,Lin Li,Zhiqiang Kong,Noel Gregoire,Rui Quan,Zisheng Luo,Xingyu Lin,Jesus Simal-Gandara,Bei Fan,Fengzhong Wang. 2023

[12]Comparison of transcriptome and metabolome analysis revealed differences in cold resistant metabolic pathways in different apple cultivars under low temperature stress. Xu, Gongxun,Li, Lijie,Zhou, Jia,Lyu, Deguo,Zhao, Deying,Qin, Sijun. 2023

[13]Combining quantitative trait locus mapping with multiomics profiling reveals genetic control of corn leaf aphid (Rhopalosiphum maidis) resistance in maize. Wang, Tengyue,Wang, Kaiji,Wang, Chuanhong,Zhao, Yibing,Tao, Zhen,Li, Junyao,Wang, Lei,Shi, Jian,Huang, Shijie,Xie, Chuanxiao,Li, Peijin. 2023

[14]Editorial: Omics data-based identification of plant specialized metabolic genes. Peipei Wang,Pengxiang Fan,Yan Bao,Wei Li,Li Wang. 2023

[15]Metabolome and Transcriptome Analyses Reveal the Differences in the Molecular Mechanisms of Oat Leaves Responding to Salt and Alkali Stress Conditions. Bai, Jianhui,Lu, Peina,Li, Feng,Li, Lijun,Yin, Qiang. 2023

[16]Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development. Chengsheng Gong,Weinan Diao,Hongju Zhu,Muhammad Jawad Umer,Shengjie Zhao,Nan He,Xuqiang Lu,Pingli Yuan,Muhammad Anees,Dongdong Yang,M. O. Kaseb,Wenge Liu. 2021

[17]The multi-omics basis of potato heterosis. Dawei Li,Xiaoyue Lu,Yanhui Zhu,Jun Pan,Shaoqun Zhou,Xinyan Zhang,Guangtao Zhu,Yi Shang,Sanwen Huang,Chunzhi Zhang. 2022

[18]Multi-Omic Analysis in a Metabolic Syndrome Porcine Model Implicates Arachidonic Acid Metabolism Disorder as a Risk Factor for Atherosclerosis. Song Song Xu,Xiu Ling Zhang,Sha Sha Liu,Shu Tang Feng,Guang Ming Xiang,Chang Jiang Xu,Zi Yao Fan,Kui Xu,Nan Wang,Yue Wang,Jing Jing Che,Zhi Guo Liu,Yu Lian Mu,Kui Li. 2022

[19]Transcriptomic and Metabolomic Analyses Provide Insights Into an Aberrant Tissue of Tea Plant (Camellia sinensis). Ding Ding Liu,Jun Ya Wang,Rong Jin Tang,Jie Dan Chen,Zhen Liu,Liang Chen,Ming Zhe Yao,Chun Lei Ma. 2021

[20]Transcription profile analysis for biosynthesis of flavor volatiles of Tunisian soft-seed pomegranate arils. Lei Yuan,Yurou Yun,Jun Tian,Zheng Qing Gao,Zhenzhen Xu,Xiaojun Liao,Junjie Yi,Shengbao Cai,Linyan Zhou. 2022

作者其他论文 更多>>