数字农科院2.0

Gibberellin modulates the post-transcriptional regulation of CsHDZ34 to positively regulate bud break in tea plants

文献类型： 外文期刊

作者： Huang, Chao;Tang, Junwei;Sun, Wenqing;Huang, Mengmeng;Chen, Yao;Zhang, Yongheng;Li, Hanjia;Chen, Weihui;Wang, Lu;Li, Nana;Ding, Changqing;Zeng, Jianming;Yang, Yajun;Wang, Yuchun;Wang, Xinchao;Hao, Xinyuan

作者机构：

关键词： alternative splicing;bud sprouting;DELLA protein;gibberellin;post-transcriptional regulation;tea plant

期刊名称： NEW PHYTOLOGIST

ISSN： 0028-646X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Sprouting, an important process in plant growth and development, is economically critical for the tea plant (Camellia sinensis (L.) O. Kuntze) and other horticultural crops, in which tender spring shoots constitute the primary harvest. While gibberellin's (GA) role in plant growth is well-established, its molecular control over bud sprouting and the underlying regulatory network remain incompletely characterized. This study employed electrophoretic mobility shift assay, dual-luciferase reporter assays, yeast one-/two-hybrid, and in situ hybridization to elucidate the transcriptional regulatory network among CsHDZ34, CsZHD9, CsDELLA5, and CsMADS27, while the functions of CsHDZ34 and CsDELLA5 in tea bud sprouting were investigated using western blotting, antisense oligodeoxynucleotides, gene overexpression, and data-independent acquisition quantitative proteomics, along with genome-wide association study analysis and high-throughput mutation detection to characterize their genetic variations. This study identified CsHDZ34 as an upstream regulator of CsZHD9, which promotes tea bud sprouting, and found that GA promoted alternative splicing in the 5 ' untranslated region of CsHDZ34, increasing its protein abundance in tea buds. The positive role of CsHDZ34 in bud break regulation was validated by ectopic expression in poplars and gene silencing in tea plants. CsDELLA5 was identified as being directly downstream of CsHDZ34 and as an interaction factor of CsMADS27. Notably, the CsDELLA5Hap2 variant was identified as less susceptible to GA-mediated degradation, allowing it to accumulate and act as a transcriptional activator that interacts with CsMADS27, thereby promoting bud sprouting through direct regulation of downstream target genes. These findings reveal a novel molecular mechanism orchestrated by CsHDZ34 that promotes bud sprouting in tea plants through spliceosome modulation and GA signaling. This provides a theoretical framework for elucidating bud break mechanisms and advancing early cultivar breeding.

分类号：

  • 相关文献

[1]Evolutionary and functional characterization of tea plant DELLA proteins. Chao Huang,Changqing Ding,Junwei Tang,Wenqing Sun,Yongheng Zhang,Yao Chen,Mengmeng Huang,Hanjia Li,Xinchao Wang,Jizhong Yu,Yuchun Wang,Xinyuan Hao. 2025

[2]A survey of transcriptome complexity using full-length isoform sequencing in the tea plant Camellia sinensis. Ma, Dongna,Fang, Jingping,Ding, Qiansu,Wei, Liufeng,Li, Yiying,Zhang, Liwen,Zhang, Xingtan. 2022

[3]MechanismofSolanumrostratumDunalSeedGermination. 杨龙,魏守辉,HongjuanHuang,ZhaofengHuang,JingchaoChen,JinyiChen,张朝贤. 2013

[4]Uncovering the complex regulatory network of spring bud sprouting in tea plants: insights from metabolic, hormonal, and oxidative stress pathways. Junwei Tang,Yao Chen,Chao Huang,Congcong Li,Yue Feng,Haoqian Wang,Changqing Ding,Nana Li,Lu Wang,Jianming Zeng,Yajun Yang,Xinyuan Hao,Xinchao Wang. 2023

[5]Evolution of plant microRNA gene families. Li, Aili,Mao, Long. 2007

[6]Expressional divergence of insect GOX genes: From specialist to generalist glucose oxidase. Wang, Xiongya,Gu, Shaohua,Li, Xianchun,Yang, Lihong,Bai, Sufen,Li, Xin,Li, Xianchun,Li, Xianchun,Wang, Chen-Zhu.

[7]BMO-MIR-9A DOWN REGULATES THE EXPRESSION OF BM-ASE GENE IN VITRO. Song, Fei,Huang, Yong,Wang, Xin,Tang, Shunming,Shen, Xingjia,Shen, Xingjia,Huang, Yong.

[8]The long non-coding RNA PILNCR2 increases low phosphate tolerance in maize by interfering with miRNA399-guided cleavage of ZmPHT1s. Wang, Yafei,Wang, Zhonghua,Du, Qingguo,Wang, Kai,Zou, Chunqin,Li, Wen-Xue. 2023

[9]Jasmonate-responsive MdMYC2/MdMED25 complex regulates malic acid accumulation in apples through the miR858-MdMYB73 module. Zhang, Bo,Huang, Zhen-Yu,Wang, Zi-Dun,Li, Guo-Fang,Hu, Gui-Bing,Yang, Ya-Zhou,Zhao, Zheng-Yang. 2025

[10]Gibberellin stimulates regrowth after defoliation of sheepgrass (Leymus chinensis) by regulating expression of fructan-related genes. Cai, Yueyue,Liu, Gongshe,Chen, Shuangyan,Shao, Linhui,Li, Xiuqing.

[11]A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.). Wang, Di,Chen, Xiaobo,Song, Gaoyuan,Kong, Xingchen,Geng, Shuaifeng,Yang, Jiayue,Wang, Bingnan,Wu, Liang,Li, Aili,Mao, Long,Zhang, Zenglin,Liu, Danmei.

[12]Gibberellins regulate the stem elongation rate without affecting the mature plant height of a quick development mutant of winter wheat (Triticum aestivum L.). Zhang, Ning,Xie, Yong-Dun,Guo, Hui Jun,Zhao, Lin-Shu,Xiong, Hong-Chun,Gu, Jia-Yu,Li, Jun-Hui,Zhao, Zi-Wei,Zhao, Shi-Rong,Liu, Lu-Xiang,Kong, Fu-Quan,Sui, Li.

[13]The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice. Qiao, Feng,Zhao, Kai-Jun,Qiao, Feng,Qiao, Feng,Zhao, Kai-Jun.

[14]Overexpression of soybean GmCBL1 enhances abiotic stress tolerance and promotes hypocotyl elongation in Arabidopsis. Li, Zhi-Yong,Xu, Zhao-Shi,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi,Li, Zhi-Yong,He, Guang-Yuan,Yang, Guang-Xiao.

[15]Gibberellin A(3) pretreatment increased antioxidative capacity of cucumber radicles and hypocotyls under suboptimal temperature. Li, Qingzhu,Li, Chaohan,Shi, Qinghua,Yu, Xianchang. 2011

[16]Tissue-specific expression and drought responsiveness of cell-wall invertase genes of rice at flowering. Ji, XM,Raveendran, M,Oane, R,Ismail, A,Lafitte, R,Bruskiewich, R,Cheng, SH,Bennett, J.

[17]EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice. Anding Luo , Qian Qian +, Hengfu Yin , Xiaoqiang Liu , Changxi Yin , Ying Lan , Ying Lan , Jiuyou Tang , Zuoshun Tang , Shouyun Cao , Xiujie Wang , Kai Xia , Xiangdong Fu , Da Luo *, Chengcai Chu *. 2006

[18]Characterizations and molecular mapping of a novel dominant semi-dwarf gene Sdd(t) in rice (Oryza sativa). B. M. Liu , Y. J. Wu *, X. D. Fu , Q. Qian. 2008

[19]Integration of Transcriptomic and Metabolomic Profiles Provides Insights into the Influence of Nitrogen on Secondary Metabolism in Fusarium sacchari. Yixue Bao,Zhenyue Lin,Wei Yao,Sehrish Akbar,Wenfeng Lin,Charles A. Powell,Jianlong Xu,Muqing Zhang. 2023

[20]OsFBK4, a novel GA insensitive gene positively regulates plant height in rice (Oryza Sativa L.). Workie Anley Zegeye,Daibo Chen,Md Anowerul Islam,Hong Wang,Aamir Riaz,Mohammad Hasanuzzaman Rani,Kashif Hussain,Qunen Liu,Xiaodeng Zhan,Shihua Cheng,Liyong Cao,Yingxin Zhang. 2022

作者其他论文 更多>>