数字农科院2.0

The Pharmacodynamics Study of Insect Defensin DLP4 Against Toxigenic Staphylococcus hyicus ACCC 61734 in Vitro and Vivo

文献类型: 外文期刊

作者: Ma Xuanxuan;Yang Na;Mao Ruoyu;Hao Ya;Yan Xue;Teng Da;Wang Jianhua

作者机构:

关键词: antibacterial mechanism; in vitro antibacterial activity; in vivo therapeutic effect; insect defensin DLP4; S. hyicus

期刊名称: Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988

年卷期: 2021 年 11 卷

页码:

收录情况: JCR(2021版)

摘要: Staphylococcus hyicus (S. hyicus), as the main pathogen of exudative epidermitis (EE) in piglet, can cause a wide variety of diseases, ranging from bovine mastitis, chicken arthritis and even human sepsis, which has brought serious threats to animals and human. The potential threat of S. hyicus infection to both public and animal health has aroused great concern. The aim of our study was to explore the efficacy of insect defensin DLP4 against S. hyicus ACCC 61734 in vitro and in vivo. The in vitro efficacies of DLP4 against S. hyicus ACCC 61734 showed high antibacterial activity (0.92 μM), a long postantibiotic effect (9.54 h), a synergistic effect with ceftriaxone, penicillin and amoxicillin, a stable bacteriostatic effect, and intracellular bacteriostatic activity against S. hyicus ACCC 61734 in HaCaT cells. Besides, the antibacterial mechanism of DLP4 against S. hyicus ACCC 61734 was explored for the first time, which indicated that the antibacterial effect of DLP4 was related to its ability to destroy cell wall and generate membrane vesicles. The in vivo therapeutic effect of DLP4 was evaluated through mouse abscess model, and the results showed that DLP4 could effectively alleviate the mouse skin abscess by inhibiting bacterial proliferation and regulating cytokines. This study first demonstrated that DLP4 may be a promising therapeutic agent against S. hyicus ACCC 61734 infection. © Copyright © 2021 Ma, Yang, Mao, Hao, Yan, Teng and Wang.

分类号:

  • 相关文献

[1]Design and pharmacodynamics of recombinant fungus defensin nzl with improved activity against staphylococcus hyicus in vitro and in vivo. Liu He,Yang Na,Teng Da,Mao Ruoyu,Hao Ya,Ma Xuanxuan,Wang Jianhua. 2021

[2]Extract of Syringa oblata: A new biocontrol agent against tobacco bacterial wilt caused by Ralstonia solanacearum. Bai, Wanming,Kong, Fanyu,Zhang, Chengsheng,Bai, Wanming,Lin, Yong.

[3]Efficient antibacterial agents: A review of the synthesis, biological evaluation and mechanism of pleuromutilin derivatives. Shang, Ruofeng,Guo, Wenzhu,Liang, Jianping,Wang, Jiatu.

[4]Mechanism of action of the tri-hybrid antimicrobial peptide LHP7 from lactoferricin, HP and plectasin on Staphylococcus aureus. Xi, Di,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Xiaojie,Wang, Jianhua,Xi, Di,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Xiaojie,Wang, Jianhua,Xi, Di.

[5]Inhibitory Effect of Camptothecin against Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp avenae RS-2. Dong, Qiaolin,Cai, Li,Hou, Mingsheng,Luo, Ju,Qiu, Wen,Anjum, Syed Ishtiaq,Li, Bin,Xie, Guanlin,Anjum, Syed Ishtiaq,Sun, Guochang.

[6]Novel xanthone antibacterials: Semi-synthesis, biological evaluation, and the action mechanisms. Yan Lu,Ting Guan,Shaobing Wang,Cui Zhou,Meizhu Wang,Xiaoyang Wang,Keyu Zhang,Xiangan Han,Jinchao Lin,Qun Tang,Chunmei Wang,Wen Zhou. 2023

[7]Copper Clusters: An Effective Antibacterial for Eradicating Multidrug‐Resistant Bacterial Infection In Vitro and In Vivo. Xiangchun Zhang,Zhichao Zhang,Qingming Shu,Chao Xu,Qinqin Zheng,Zhao Guo,Chen Wang,Zhenxia Hao,Xin Liu,Guoqing Wang,Wangjun Yan,Hongping Chen,Chengyin Lu. 2021

[8]Inhibition of Haemophilus parasuis by berberine and proteomic studies of its mechanism of action. Yue Jia,Changqi Hao,Qin Yang,Wanjiang Zhang,Gang Li,Siguo Liu,Xin Hua. 2021

[9]Discovery of 1’-acetoxychavicol acetate (ACA) as a promising antibacterial compound from galangal (Alpinia galanga (Linn.) Willd). Dan Zhang,Liang Zou,Ding Tao Wu,Qi Guo Zhuang,Hua Bin Li,Vuyo Mavumengwana,Harold Corke,Ren You Gan. 2021

[10]Harsh Sensitivity and Mechanism Exploration of an Antibacterial Peptide Extracted from Walnut Oil Residue Derived from Agro-Industrial Waste. Dongwei Liu,Mei Liu,Dehao Meng,Yihan Mu,Tao Wang,Zhaolin Lv. 2022

[11]Antibacterial Activity of Chinese Red Propolis against Staphylococcus aureus and MRSA. Wenwen Zhang,Gomez Escalada Margarita,Di Wu,Wenqin Yuan,Sha Yan,Suzhen Qi,Xiaofeng Xue,Kai Wang,Liming Wu. 2022

[12]In Vitro Antimicrobial Synergistic Activity and the Mechanism of the Combination of Naringenin and Amikacin Against Antibiotic-Resistant Escherichia coli. Lankun Yi,Mingze Cao,Xu Chen,Yubin Bai,Weiwei Wang,Xiaojuan Wei,Yuxiang Shi,Yongying Zhang,Tenghe Ma,Zhen Zhu,Jiyu Zhang. 2024

[13]Biosynthesis of high antibacterial silver chloride nanoparticles against Ralstonia solanacearum using spent mushroom substrate extract. Wenjing Mo,Chunmei Yao,Hongsen Chen,Aisha Khalfan Nassor,Fangze Gui,Ciqing Hong,Tianpei Huang,Xiong Guan,Lei Xu,Xiaohong Pan. 2024

[14]Preservation mechanism of chitosan/gelatin films modified by deep eutectic solvent on chilled chicken breast at 4 °C storage. Daobang Tang,Haitao Wen,Zhijie Xu,Jinhao Zou,Xueming Liu,Dequan Zhang,Xuping Wang. 2025

[15]Glabridin pharmacodynamic activity toward Gram-positive bacterial infections. Chan Chen,Rui Sun,Tianle Pan,Lian Liu,Wenqiang Tang,Mi Wang,Lifang Zhang,Chenzhong Fei,Yingchun Liu. 2025

[16]Advances in antibacterial strategies of organic acids coupled with cold sterilization methods for controlling bacterial contamination in beef carcasses and cuts. Yu, Haojie,Liu, Xiaochang,Zhang, Songshan,Lei, Yuanhua,Zou, Bo,Xie, Peng,Sun, Baozhong,Zang, Mingwu. 2025

[17]Research Progress on the Antibacterial Activity of Natural Flavonoids. Zhijin Zhang,Mingze Cao,Zixuan Shang,Jing Xu,Xu Chen,Zhen Zhu,Weiwei Wang,Xiaojuan Wei,Xuzheng Zhou,Yubin Bai,Jiyu Zhang. 2025

[18]Personalized design enzyme-like activity theanine coated Pt clusters for catalytic damage of bacterial and infection management. Chao Xu,Juanjuan Guo,Qinqin Zheng,Mingchuan Yang,Zhenduo Han,Na Zhang,Chunyu Zhang,Hongping Chen,Xiangchun Zhang. 2025

[19]Control mechanisms of ε-polylysine and EDTA against Pectobacterium aroidearum isolated from rotted pickled red pepper fruits. Yang, Shengbo,Hu, Jingjing,Chen, Qingmin,Liu, Hu,Du, Yamin,Zhang, Zhanquan,Xin, Xiaofei,Fu, Maorun. 2025

[20]Novel Antibiotic-Derived Silicon Dots with Broad-Spectrum Antibacterial Activity and Efficient Wound Healing Promotion. Mao, Guobin,Yin, Wen,Yang, Yeling,Zeng, Yuan,Zhou, Jie,Li, Jia-Ao,Wan, Yanhui,Zhou, Wenhua,Yu, Xue-Feng,Dai, Junbiao,Ma, Yingxin. 2025

作者其他论文 更多>>