数字农科院2.0

A novel regulation on the developmental checkpoint protein Sda that controls sporulation and biofilm formation in Bacillus subtilis

文献类型： 外文期刊

作者： He, Yinghao;Qin, Yuxuan;Greenwich, Jennifer;Balaban, Samantha;Darcera, Migue Van Louis;Gozzi, Kevin;Chai, Yunrong

作者机构：

关键词： Bacillus subtilis;Sda;biofilm;sporulation;serine codon;synonymous codon

期刊名称： JOURNAL OF BACTERIOLOGY

ISSN： 0021-9193

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Biofilm formation by Bacillus subtilis is triggered by an unusually simple environmental sensing mechanism. Certain serine codons, the four TCN codons (N for A, T, C, or G), in the gene for the biofilm repressor SinR caused lowered SinR translation and subsequent biofilm induction during transition from exponential to stationary growth. Global ribosome profiling showed that ribosomes pause when translating the four UCN (U for T on the mRNA) serine codons on mRNA, but not the two AGC/AGU serine codons. We proposed a serine codon hierarchy (AGC/AGT vs TCN) in that genes enriched in the TCN serine codons may experience reduced translation efficiency when serine is limited. In this study, we designed an algorithm to score all protein-coding genes in B. subtilis NCIB3610 based on the serine codon hierarchy. We generated a short list of 50 genes that could be subject to regulation by this novel mechanism. We further investigated one such gene from the list, sda, which encodes a developmental checkpoint protein regulating both sporulation and biofilm formation. We showed that synonymously switching the TCN serine codons to AGC in sda led to delayed biofilm formation and sporulation. This engineered strain also outgrew strains with other synonymously substituted sda alleles (TCN) in competition assays for biofilm formation and sporulation. Finally, we showed that the AGC serine codon substitutions in sda elevated the Sda protein levels. This serine codon hierarchy-based novel signaling mechanism could be exploited by bacteria in adapting to stationary phase and regulating important biological processes.

分类号：

  • 相关文献

[1]Multiwalled Carbon Nanotubes Promoted Biofilm Formation and Rhizosphere Colonization of Bacillus subtilis Tpb55. Lv, Xiaolin,Liu, Shanshan,Cao, Yi,Wu, Huagen,Zhang, Chengsheng,Huang, Bin,Wang, Jie. 2025

[2]The silent point mutations at the cleavage site of 2A/2B have no effect on the self-cleavage activity of 2A of foot-and-mouth disease virus. Gao, Zong-liang,Zhou, Jian-hua,Zhang, Jie,Ding, Yao-zhong,Liu, Yong-sheng.

[3]Dual signal amplification strategy: CRISPR/Cas9 trans-cleavage combined with strand displacement amplification for influenza virus detection. Song, Ziqin,Sun, He,Zhou, Mengyuan,Bu, Shengjun,Zhou, Hongyu,Liu, Che,Hao, Zhuo,Yu, Songling,Wan, Jiayu,Tang, Feng. 2025

[4]Optimize culture conditions for biomass and sporulation of the nematophagous fungus Pochonia chlamydosporia, isolate HSY-12-14 by "two-step" cultivation. Gao, Li. 2011

[5]Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation. Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Zhu, Xing-Quan,He, Shuai,Zhou, Dong-Hui,Elsheikha, Hany M.,He, Shuai,Li, Qian.

[6]A novel two-stage cultivation method to optimize carbon concentration and carbon-to-nitrogen ratio for sporulation of biocontrol fungi. Liu, X. Z.,Gao, L..

[7]Optimization of Culture Medium for Sporulation and Biomass Production of a Nematophagous Fungus: Consideration of Nutritional and Environmental Conditions. .

[8]A NOVEL METHOD TO OPTIMIZE CULTURE CONDITIONS FOR BIOMASS AND SPORULATION OF THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA IBC1201. . 2011

[9]Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods. Liu, Xingzhong,Gao, Li.

[10]Sporulation of several biocontrol fungi as affected by carbon and nitrogen sources in a two-stage cultivation system. Liu, Xingzhong,Gao, Li.

[11]Disruption Of Two-Component System Lytsr A.ffects Forespore Engulfment In B acillus Thuringiensis. Peng, Q,Wu, JB,Chen, XM,Qiu, LL,Zhang, J,Tian, HT,Song, FP. 2017

[12]StcU-2 Gene Mutation via CRISPR/Cas9 Leads to Misregulation of Spore-Cyst Formation in Ascosphaera apis. Aynalem T.,Meng L.,Getachew A.,Wu J.,Yu H.,Tan J.,Li N.,Xu S.. 2022

[13]Identification and functional characterization of two homologous SpoVS proteins involved in sporulation of bacillus thuringiensis. Xinlu Liu,Ruibin Zhang,Shuo Hou,Huanhuan Liu,Jiaojiao Wang,Qingyue Yu,Qi Peng,Fuping Song. 2021

[14]Sporulation rate and viability of Eimeria tenella oocysts stored in potassium sorbate solution. Zhenxing Gong,Hongmei Wei,Fang Chang,Hong Yin,Jianping Cai. 2021

[15]The Transcription Factor CpcR Determines Cell Fate by Modulating the Initiation of Sporulation in Bacillus thuringiensis. Hou, Shuo,Zhang, Ruibin,Lereclus, Didier,Peng, Qi,Zhang, Jie,Slamti, Leyla,Song, Fuping. 2022

[16]Key amino acids residues enhance the ability of CpcR to activate cry gene expression in Bacillus thuringiensis. Ruibin Zhang,Yang Luo,Lili Gang,Yanrong Xu,Xin Zhang,Qi Peng,Leyla Slamti,Didier Lereclus,Guirong Wang,Fuping Song. 2023

[17]Recombinant Escherichia Coli Bl21-Pet28A-Egfp Cultivated W.ith Nanomaterials In A M odified Microchannel For Biofilm Formation. Mei, Yi-Yuan,Zhu, Chang-Tong,Sheng, Sheng,Sheng, Sheng,Xu, Yan,Wang, Jun,Wang, Jun,Xu, Yan,Zhu, Lin-Lin,Sheng, Sheng,Xu, Yan,Zhu, Chang-Tong,Zhu, Lin-Lin,Wang, Jun,Mei, Yi-Yuan. 2018

[18]Growth Enhancement and Disease Protection of Tobacco by the Volatile-producing Bacillus Subtilis Strain Tpb55. Zhang Cheng-sheng,Kong Fan-yu,Wang Jing. 2011

[19]Inactivation of Bacillus subtilis by a Pulsed Magnetic Field and Kinetics Model. Qian, Jing-ya,Ma, Hai-le,Li, Shu-jun,Cui, Feng-jie,Qu, Wen-juan,Li, Shu-jun,Qian, Jing-ya,Ma, Hai-le,Qu, Wen-juan.

[20]Proteomics Analyses and Morphological Structure of Bacillus subtilis Inactivated by Pulsed Magnetic Field. Qian, Jingya,Zhou, Cunshan,Ma, Haile,Li, Shujun,Abdualrahman, Mohammed A. Y.,Qian, Jingya,Zhou, Cunshan,Ma, Haile,Qian, Jingya,Li, Shujun,Yagoub, Abu ElGasim A.,Abdualrahman, Mohammed A. Y.. 2016

作者其他论文 更多>>