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PEDV promotes the differentiation of CD4+T cells towards Th1, Tfh, and Treg cells via CD103+DCs

文献类型： 外文期刊

作者： Haoyuan Yu;Guohui Chen;Tao Zhang;Xin Huang;Ya Bin Lu;Maolin Li;Shuxian Li;Caiying Wang;Baoyu Li;Yunhang Zhang;Guangliang Liu;Yuguang Fu

作者机构：

关键词： CD103+DCs;CD4+T differentiation;PEDV

期刊名称： Virology

ISSN： 0042-6822

年卷期： 2023 年 587 卷

页码：

收录情况： SCIE（2023版）

摘要： Porcine epidemic diarrhea virus (PEDV) can infect all ages of pigs, particularly newborn piglets with a mortality almost reaching to 80–100%, causing significant economic losses to the global pig industry. The mucosal immune response is crucial for PEDV prevention, in which specific dendritic cells (DCs) and differentiated T cells play vital roles. In this study, CD103+DCs were differentiated successfully with retinoic acid (RA) treatment in vitro. PEDV could not replicate efficiently in differentiated CD103+DCs but could promote maturation of CD103+DCs by up-regulating the expression of SLA-DR, CD1a, CD86, and cytokines of IL-1β and IL-10. In addition, PEDV-infected CD103+DCs and CD4+T cells were co-cultured, and the results showed that the differentiation of CD4+T cells toward Th1, Tfh, and Treg, but not Th2. These results demonstrate that PEDV-infected CD103+DCs could promote the differentiation of CD4+T cells, which provided the basis for further study of mucosal response induced by PEDV via CD103+DCs.

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