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Hexokinase gene CsHXK4 positively regulates cold resistance in tea plants (Camellia sinensis)

文献类型： 外文期刊

作者： Yujie Wang;Yiqian Zheng;Lu Wang;Yufan Ye;Xinbo Shen;Xinyuan Hao;Changqing Ding;Yajun Yang;Xinchao Wang;Nana Li

作者机构：

关键词： Camellia sinensis;CBF;Cold tolerance;Hexokinase;Sugar signaling

期刊名称： Plant Physiology and Biochemistry

ISSN： 0981-9428

年卷期： 2025 年 221 卷

页码：

收录情况： SCIE（2025版）

摘要： Low temperatures are a major abiotic stress factor that adversely affects the growth, development, and productivity of tea plants. Soluble sugars play a critical role in enhancing tea plants' resistance to cold stress. However, the mechanisms by which tea plants perceive and transmit sugar signals remain poorly understood. In this study, the transcription of CsHXK4, a type B hexokinase (HXK) gene in tea plants, was significantly upregulated by low temperatures and glucose signaling. CsHXK4 exhibited multiple subcellular localizations, particularly in the nucleus and mitochondria, and was ubiquitously expressed across various tissues, with predominant expression in younger organs. Hexose phosphorylation analysis using the HXK-deficient yeast triple mutant YSH7.4–3C demonstrated that CsHXK4 functions as a hexokinase with catalytic activity. Overexpression (OE) of CsHXK4 in Arabidopsis resulted in hypersensitivity to glucose, suggesting its role in sugar signal transduction. Furthermore, CsHXK4-OE Arabidopsis plants exhibited enhanced cold tolerance compared to wild-type plants, as evidenced by increased photosynthetic capacity, elevated sugar content, and stable cell membrane permeability. Transient induction of CsHXK4 expression in tea leaves improved freezing tolerance and activated the expression of cold-responsive marker genes, including CsCBF1/2/3/4, CsKIN2.2, CsCOR413, CsLEA14, and CsGOLS1, compared to the empty vector control. Overall, our findings reveal that CsHXK4 positively regulates cold resistance in tea plants by mediating sugar signaling and CBF signaling pathways.

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