数字农科院2.0

Biogenic Synthesis and Characterization of Hypecoum pendulum Mediated Silver Nanoparticles: Revealed Outstanding Anticancer and Genotoxic Potentials

文献类型： 外文期刊

作者： Ul Huda, Noor;Ahmed, Mushtaq;Mushtaq, Nadia;Badshah, Saboor;Saeed, Haleema;Khan, Rahmat Ali;Badshah, Farhad;Yassin, Mohamed Taha

作者机构：

关键词： alkaline comet assay;anticancer activity;genotoxicity;Hypecoum pendulum;nanoparticles;nanotechnology

期刊名称： JOURNAL OF APPLIED TOXICOLOGY

ISSN： 0260-437X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Fabrication of silver nanoparticles by green approach is the most effective and eco-friendly technique in recent technologies. The current study aimed to generate a simple, valid, and justifiable method for biogenic synthesis of silver nanoparticles (HP-AgNPs) using aqueous extract of Hypecoum pendulum L.(HP) and to assess their in vitro anticancer and genotoxic potentials on baby hamster kidney cell (BHK-21) and human blood lymphocytes using 3-(4,5-dimethylthiazol-2-yl-)-2,5-di-phenyltetrazolium bromide (MTT) and alkaline comet assay, respectively. HP-AgNP characterization was done using UV-vis spectrometry, EDX, SEM, XRD, and FTIR techniques. The crystalline nature of HP-AgNPs with a particle size of 36.3 nm was assessed using the XRD technique. The surface morphologies with a particle size of 80 nm were verified by SEM analysis. UV spectroscopy verified the existence of HP-AgNPs by yielding a sharp peak at 417 nm with an absorbance intensity of 1.54. FTIR assessment revealed the existence of different functional moieties that contribute to the HP-AgNPs stabilization and reduction. Similarly, EDX analysis revealed Ag as a principal element (49%). MTT assay showed significant cytotoxicity by Doxorubicin and HP-AgNPs with a smaller IC50 value of 104.21 +/- 4.33 and 134.91 +/- 6.33 mu g/mL correlated to HP extract (229.84 +/- 4.66 mu g/mL). The outcomes of the comet assay revealed potential DNA damage in a positive trend with concentration (25-600 mu g/mL). HP-AgNP-treated lymphocytes showed higher DNA damage as compared to HP extract-treated cells, but less damage as compared to a positive control, H2O2. These outcomes showed that HP-AgNPs have demonstrated promising anticancer and genotoxic action than HP extract due to their size and shape.

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