数字农科院2.0

Cell-adapted African swine fever virus Pig/HLJ/18 is highly attenuated but fails to induce immune protection against a challenge with its parental virus

文献类型： 外文期刊

作者： 王婉;;;尹丽;;张振江;;刘帆;;步志高;;朱远茂;;赵东明

关键词： 非洲猪瘟病毒

期刊名称： Journal of Integrative Agriculture

ISSN： 2095-3119

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： African swine fever (ASF) is an acute, hemorrhagic disease caused by the African swine fevervirus (ASFV), with a mortality up to 100%. The disease poses a seriously threat to the global swineindustry, yet no commercial vaccines or antiviral drugs are available other than in Vietnam. ASFVattenuation through serial passages is a key approach for vaccine development. In this study, acell-adapted virus, named HLJ18/BK33, was successfully generated by serially passaging the ASFVPig/HLJ/18 in wild boar kidney cells (BK2258). This adapted virus exhibited clear cytopathic effects(CPE) and replicated stably and efficiently in BK2258 cells and porcine alveolar macrophages. Whole-genome sequence analysis revealed that, compared with the Pig/HLJ/18 virus, HLJ18/BK33hada large deletion of 6162 bp from sites 181,027 to 187,188, and four single nucleotide deletions that ledto frameshift mutations, resulting in the truncated expression of three open reading frames (ORFs)(ASFV_G_ACD_00120, ASFV_G_ACD_00350, and A179L), and the fusion expression of twoORFs(MGF_110-14L and MGF_110-11L). Additionally, four genes exhibited missense mutations, leadingtosingle amino acid changes. Five pigs intramuscularly inoculated with 106 TCID50 of HLJ18/BK33remained healthy with normal body temperatures and no clinical signs, indicating a high attenuationofvirulence for HLJ18/BK33 in pigs. Upon challenge with the parental Pig/HLJ/18 virus, four of thefiveinoculated pigs developed persistent high fever and ASF-related clinical signs and died within13daysof the challenge; the remaining pig developed transient fever but survived until the endof the observation period. These results indicate that the HLJ18/BK33 virus is highly attenuated but cannotinduce protection against the parental virulent virus. Even though the HLJ18/BK33 virus is not agoodvaccine candidate, its stable replication and distinct CPE in BK2258 cells as well as its lowbiosafetyrisk make it a valuable resource for studies on virus-host interactions, antiviral drug screening, diagnostic methods, and biological characteristics.

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