数字农科院2.0

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus

文献类型： 外文期刊

作者： Yuying Cao;Li Yuan;Shunli Yang;Youjun Shang;Bin Yang;Zhizhong Jing;Huichen Guo;Shuanghui Yin

作者机构：

关键词： antibody detection;blocking ELISA;Classical swine fever virus;E2 protein

期刊名称： Journal of veterinary science

ISSN： 1976-555X

年卷期： 2022 年 23.0 卷 5 期

页码：

收录情况： SCIE（2022版）

摘要： BACKGROUND: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. OBJECTIVES: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. METHODS: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. RESULTS: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. CONCLUSIONS: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

分类号：

  • 相关文献

[1]A Customized Novel Blocking ELISA for Detection of Bat-Origin Swine Acute Diarrhea Syndrome Coronavirus Infection. Cao, Liyan,Kong, Xiangyu,Li, Xiangtong,Suo, Xuepeng,Duan, Yueyue,Yuan, Cong,Zhang, Yu,Zheng, Haixue,Wang, Qi. 2023

[2]Development of a blocking ELISA employing a VP1-specific monoclonal antibody for the detection of DHAV3 antibodies. Cuiping Song,Tianyuan Li,Jing Wang,Peidong Guo,Wenjing Yang,Ning Tang,Yang Qu,Siyu Li,Xusheng Qiu,Lei Tan,Yingjie Sun,Ying Liao,Chan Ding. 2025

[3]Specific ligands for classical swine fever virus screened from landscape phage display library. Yin, Long,Luo, Yuzi,Du, Min,Qiu, Hua-Ji,Yin, Long,Liang, Bo,Wang, Fei,Liu, Aihua,Yin, Long,Wang, Fei,Liu, Aihua,Petrenko, Valery A..

[4]Identification of two novel T cell epitopes on the E2 protein of classical swine fever virus C-strain. Xiaotian Zhao,Xiao Wang,Mengqi Yuan,Xin Zhang,Xiaoke Yang,Xiangyu Guan,Shuwen Li,Jifei Ma,Hua Ji Qiu,Yongfeng Li. 2023

[5]Ferritin Nanoparticle Delivery of the E2 Protein of Classical Swine Fever Virus Completely Protects Pigs from Lethal Challenge. Dailang Zhong,Zhanhao Lu,Yu Xia,Hongxia Wu,Xinyu Zhang,Mingzhi Li,Xin Song,Yanjin Wang,Assad Moon,Hua Ji Qiu,Yongfeng Li,Yuan Sun. 2024

[6]A recombinant pseudorabies virus surface - displaying the classical swine fever E2 protein induces specific antibodies rapidly. Zhang, Xinyu,Wu, Hongxia,Gao, Tianqi,Li, Yongfeng,Zhong, Dailang,Li, Mingzhi,Li, Shuwen,Ma, Caoyuan,Moon, Assad,Fu, Qiang,Qiu, Hua-Ji,Sun, Yuan. 2024

[7]Analysis of murine B-cell epitopes on Eastern equine encephalitis virus glycoprotein E2. Sun EnCheng,Zhao Jing,Yang Tao,Xu QingYuan,Qin YongLi,Wang WenShi,Wei Peng,Sun Liang,Sun Jing,Wu DongLai. 2013

[8]Development and application of an indirect ELISA for detection of antibodies against emerging atypical porcine pestivirus. Hao Song,Xiaowei Gao,Jing Li,Xinying Dong,Yanhui Fu,Lina Shao,Jiaoer Zhang,Hua Ji Qiu,Yuzi Luo. 2024

[9]Expression of BVDV E2 protein in CHO-S cells and development of an indirect ELISA for serological detection. Jiahui Wang,Ao Zhang,Zhiwei Hou,Bin Tan,Shuqin Zhang. 2025

[10]Lipid nanoparticle encapsulated membrane-anchored E2 mRNA vaccine elicits cross-protective immune responses against bovine viral diarrhoea virus infection in calves. Ting Le,Bin Jia,Chao Sun,Yongfei Zhou,Yinglin Qi,Hao Liu,Haiqiao Bian,Chongyu Tian,Jun Wang,Fei Xue,Yuanmao Zhu,Jitao Chang,Yunfei Luan,Zexin Zhang,Zhendong Li,Wanbo Tai,Zhigang Jiang,Xin Yin. 2025

[11]A single-dose mRNA vaccine encoding the classical swine fever virus E2-ECD induces durable protective immunity in rabbits. Bian, Li-Jun,Tang, Yu,Yang, Fan,Tian, Hong,Peng, Qin,Tang, Ming-Liang,Chen, Yi-Zhen,Xia, Tian,Li, Shu,Zheng, Hai-Xue,Shu, Hong-Bing,Li, Mi. 2025

[12]E2-based mRNA vaccine encapsulated in lipid nanoparticles protects pigs against classical swine fever virus. Liu, Jingyi,Xia, Yingju,Tian, Chuanwen,Chen, Ziyu,Guo, Weiqiang,Liu, Yingnan,Wen, Jing,Xie, Zhenhua,Lin, Jinzhong,Li, Jiaxin,Chen, Hongjun,Liu, Yebing. 2025

[13]Development of blocking ELISA for detection of antibodies against H9N2 avian influenza viruses. Li, Zejun.

[14]Development of a blocking ELISA for detection of antibodies against avian hepatitis E virus. Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhang, Gaiping,Zhou, En-Min,Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhou, En-Min,Zhang, Gaiping,Luo, Jianxuri,Hsu, Walter H..

[15]Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2. Huang, Liping,Lu, Yuehua,Wei, Yanwu,Guo, Longjun,Liu, Changming. 2011

[16]Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus. Gao Y.,Xia T.,Bai J.,Zhang L.,Zheng H.,Jiang P.. 2022

[17]Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia. Hu, Zhe,Guo, Kui,Du, Cheng,Sun, Jinhui,Naletoski, Ivancho,Chu, Xiaoyu,Lin, Yuezhi,Wang, Xuefeng,Barrandeguy, Maria,Samuel, Mino,Wang, Wen,Lau, Patrick Imtung,Wernery, Ulrich,Raghavan, Rekha,Wang, Xiaojun. 2023

[18]Novel P22-monoclonal antibody based blocking ELISA for the detection of African swine fever virus antibodies in serum. Tsegay G.,Tesfagaber W.,Zhu Y.,He X.,Wang W.,Zhang Z.,Sun E.,Zhang J.,Guan Y.,Li F.,Liu R.,Bu Z.,Zhao D.. 2022

[19]Improved detection sensitivity of anti-PRV variant antibodies through preparation of anti-gB and anti-gE monoclonal antibodies and development of blocking ELISAs. Zhenyang Guo,Hu Xu,Siyu Zhang,Haonan Kang,Chao Li,Qi Sun,Jing Zhao,Jinhao Li,Guohui Zhou,Qian Wang,Lirun Xiang,Yandong Tang,Huairan Liu,Chaoliang Leng,Tongqing An,Xuehui Cai,Zhijun Tian,Hongliang Zhang,Jinmei Peng. 2024

[20]A highly efficient blocking ELISA based on p72 monoclonal antibody for the detection of African swine fever virus antibodies and identification of its linear B cell epitope. Weldu Tesfagaber,Wan Wang,Lulu Wang,Rui Zhao,Yuanmao Zhu,Fang Li,Encheng Sun,Renqiang Liu,Zhigao Bu,Geng Meng,Dongming Zhao. 2024

作者其他论文 更多>>