数字农科院2.0

Rational design of ROS scavenging and fluorescent gold nanoparticles to deliver siRNA to improve plant resistance to Pseudomonas syringae

文献类型： 外文期刊

作者： Jie Qi;Yanhui Li;Xue Yao;Guangjing Li;Wenying Xu;Lingling Chen;Zhouli Xie;Jiangjiang Gu;Honghong Wu;Zhaohu Li

作者机构：

关键词： （0-1-5）Disease resistance;Fluorescent gold nanoparticles;Gene silencing;RNA delivering;ROS scavenging ability

期刊名称： Journal of Nanobiotechnology

ISSN： 1477-3155

年卷期： 2024 年 22 卷 1 期

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Bacterial diseases are one of the most common issues that result in crop loss worldwide, and the increasing usage of chemical pesticides has caused the occurrence of resistance in pathogenic bacteria and environmental pollution problems. Nanomaterial mediated gene silencing is starting to display powerful efficiency and environmental friendliness for improving plant disease resistance. However, the internalization of nanomaterials and the physiological mechanisms behind nano-improved plant disease resistance are still rarely understood. We engineered the polyethyleneimine (PEI) functionalized gold nanoparticles (PEI-AuNPs) with fluorescent properties and ROS scavenging activity to act as siRNA delivery platforms. Besides the loading, protection, and delivery of nucleic acid molecules in plant mature leaf cells by PEI-AuNPs, its fluorescent property further enables the traceability of the distribution of the loaded nucleic acid molecules in cells. Additionally, the PEI-AuNPs-based RNAi delivery system successfully mediated the silencing of defense-regulated gene AtWRKY1. Compared to control plants, the silenced plants performed better resistance to Pseudomonas syringae, showing a reduced bacterial number, decreased ROS content, increased antioxidant enzyme activities, and improved chlorophyll fluorescence performance. Our results showed the advantages of AuNP-based RNAi technology in improving plant disease resistance, as well as the potential of plant nanobiotechnology to protect agricultural production.

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