数字农科院2.0

A transcriptomic atlas of Aedes aegypti reveals detailed functional organization of major body parts and gut regional specializations in sugar-fed and blood-fed adult females

文献类型： 外文期刊

作者： Hixson, Bretta;Bing, Xiao-Li;Yang, Xiaowei;Bonfini, Alessandro;Nagy, Peter;Buchon, Nicolas

作者机构：

关键词： Aedes;Anopheles;RNAseq atlas;gut transcriptome;blood feeding;innate immunity;Aedes aegypti;Anopheles gambiae

期刊名称： ELIFE

ISSN： 2050-084X

年卷期： 2022 年 11 卷

页码：

收录情况： SCIE（2022版）

摘要： Mosquitoes transmit numerous pathogens, but large gaps remain in our understanding of their physiology. To facilitate explorations of mosquito biology, we have created Aegypti-Atlas (), an online resource hosting RNAseq profiles of Ae. aegypti body parts (head, thorax, abdomen, gut, Malpighian tubules, ovaries), gut regions (crop, proventriculus, anterior and posterior midgut, hindgut), and a gut time course of blood meal digestion. Using Aegypti-Atlas, we provide insights into regionalization of gut function, blood feeding response, and immune defenses. We find that the anterior and posterior midgut possess digestive specializations which are preserved in the blood-fed state. Blood feeding initiates the sequential induction and repression/depletion of multiple cohorts of peptidases. With respect to defense, immune signaling components, but not recognition or effector molecules, show enrichment in ovaries. Basal expression of antimicrobial peptides is dominated by holotricin and gambicin, which are expressed in carcass and digestive tissues, respectively, in a mutually exclusive manner. In the midgut, gambicin and other effectors are almost exclusively expressed in the anterior regions, while the posterior midgut exhibits hallmarks of immune tolerance. Finally, in a cross-species comparison between Ae. aegypti and Anopheles gambiae midguts, we observe that regional digestive and immune specializations are conserved, indicating that our dataset may be broadly relevant to multiple mosquito species. We demonstrate that the expression of orthologous genes is highly correlated, with the exception of a 'species signature' comprising a few highly/disparately expressed genes. With this work, we show the potential of Aegypti-Atlas to unlock a more complete understanding of mosquito biology.

分类号：

  • 相关文献

[1]The transcriptional response in mosquitoes distinguishes between fungi and bacteria but not Gram types. Hixson B.,Huot L.,Morejon B.,Yang X.,Nagy P.,Michel K.,Buchon N.. 2024

[2]Dibenzo-alpha-pyrones: a new class of larvicidal metabolites against Aedes aegypti from the endophytic fungus Hyalodendriella sp Ponipodef12. Mao, Ziling,Lai, Daowan,Liu, Xunda,Fu, Xiaoxiang,Meng, Jiajia,Wang, Ali,Wang, Xiaohan,Sun, Weibo,Zhou, Ligang,Liu, Zhi Long,Liu, Yang.

[3]Insight into Hyalomma anatolicum biology by comparative genomics analyses. Wang, Jinming,Chai, Yijun,Yang, Jifei,Chen, Kai,Liu, Guangyuan,Luo, Jianxun,Guan, Guiquan,Ren, Qiaoyun,Yin, Hong. 2024

[4]Identification of chemosensory protein inhibitors as a potential strategy to combat pyrethroid resistance in Anopheles gambiae. Liu, Chen,Sundarraj, Rajamanikandan,Yang, Ning,Dong, Meijing,Yuchi, Zhiguang. 2025

[5]Newcastle disease virus-like particles induce DC maturation through TLR4/NF-kappa B pathway and facilitate DC migration by CCR7-CCL19/CCL21 axis. Qian, Jing,Xu, Xiaohong,Ding, Jiaxin,Yin, Renfu,Xue, Cong,Cong, Yanlong,Ding, Zhuang,Sun, Yixue,Wang, Jianzhong,Wang, Chunfeng,Ding, Chan,Yu, Shengqing,Liu, Xiufan,Hu, Shunlin.

[6]Transcript Profiling of Toll-Like Receptor mRNAs in Selected Tissues of Mink (Neovison vison). Tong, Mingwei,Yi, Li,Cheng, Yuening,Zhang, Miao,Cao, Zhigang,Wang, Jianke,Zhao, Hang,Lin, Peng,Cheng, Shipeng,Yang, Yong.

[7]Two myeloid differentiation factor 88 (MyD88) isoforms identified in ducks. Cheng, Yuqiang,Wang, Hengan,Yan, Yaxian,Sun, Jianhe,Ding, Chan.

[8]Molecular cloning and functional characterization of a novel isoform of chicken myeloid differentiation factor 88 (MyD88). Qiu, Yafeng,Shen, Yang,Li, Xiangdong,Ding, Chan,Ma, Zhiyong.

[9]A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity. Zhou, Jinlin,Ueda, Mami,Umemiya, Rika,Battsetseg, Badgar,Boldbaatar, Damdinsuren,Xuan, Xuenan,Fujisaki, Kozo.

[10]Icariin induces the expression of toll-like receptor 9 in Ana-1 murine macrophages. Li, Li,Miao, Jianhua,Xu, Yongli,Peng, Lina,Qiu, Yafeng,Gao, Xiaoqi,Shi, Zixue,Shao, Donghua,Ma, Zhiyong,Zhou, Ying.

[11]Molecular cloning of Peking duck Toll-like receptor 3 (duTLR3) gene and its responses to reovirus infection. Song, Kaijie,Li, Chuanfeng,Chen, Zongyan,Ding, Chan,Liu, Guangqing,Zhang, Miaotao,Song, Kaijie. 2015

[12]Regulatory effects of dietary L-Arg supplementation on the innate immunity and antioxidant ability in broiler chickens. Hu Ya-di,Tan Jian-zhuang,Qi Ji,Zhang Hong-fu. 2016

[13]Goose RIG-I functions in innate immunity against Newcastle disease virus infections. Sun, Yingjie,Ding, Na,Yu, Shengqing,Meng, Chunhun,Chen, Hongjun,Qiu, Xusheng,Zhang, Shilei,Yu, Yang,Zhan, Yuan,Ding, Chan,Ding, Siyu Serena.

[14]Glycyrrhetinic acid extracted from Glycyrrhiza uralensis Fisch induces the expression of Toll-like receptor 4 in Ana-1 murine macrophages. Li, Li,Miao, Jian-Hua,Xu, Yong-Li,Peng, Li-Na,Qiu, Ya-Feng,Gao, Xiao-Qi,Shi, Zi-Xue,Shao, Dong-Hua,Wei, Jian-Chao,Ma, Zhi-Yong,Zhou, Ying. 2011

[15]Differential and spatial regulation of the prophenoloxidase (proPO) and proPO-activating enzyme in cuticular melanization and innate immunity in Bombyx mori pupae. Zou, Feng Ming,Lee, Kwang Sik,Kim, Bo Yeon,Jin, Byung Rae,Zou, Feng Ming,Kim, Hong Ja,Gui, Zhong Zheng,Zhang, Guo Zheng,Guo, Xijie. 2015

[16]Peptidoglycan Recognition Protein S2 From Silkworm Integument: Characterization, Microbe-Induced Expression, and Involvement in the Immune-Deficiency Pathway. Yang, Jie,Wang, Xiaonan,Wu, Jinmei,Tang, Shunming,Shen, Zhongyuan,Wu, Jinmei. 2015

[17]Recent Progress in Understanding PAMP- and Effector-Triggered Immunity against the Rice Blast Fungus Magnaporthe oryzae. Liu, Jinling,Ning, Yuese,Ding, Bo,Wang, Xuli,Wang, Guo-Liang,Liu, Jinling,Wang, Zhilong,Liu, Jinling,Wang, Zhilong,Wang, Guo-Liang. 2013

[18]The VP3 structural protein of foot-and-mouth disease virus inhibits the IFN-beta signaling pathway. Li, Dan,Yang, Wenping,Yang, Fan,Liu, Huanan,Zhu, Zixiang,Lian, Kaiqi,Liu, Xiangtao,Zheng, Haixue,Lei, Caoqi,Li, Shu,Shu, Hongbing.

[19]The role of effectors and host immunity in plant-necrotrophic fungal interactions. Jiang, Nan,Liu, Wende,Wang, Guo-Liang,Jiang, Nan,Liu, Jinling,Jiang, Nan,Liu, Jinling,Wang, Guo-Liang.

[20]The Tug-Of-War Between Plants And V.iruses: Great Progress And M any Remaining Questions. Cheng, Xiaofei,Zhou, Xueping,Zhou, Xueping,Wu, Xiaoyun,Valli, Adrian,Antonio Garcia, Juan. 2019

作者其他论文 更多>>