数字农科院2.0

Knockout of the EgriBLOS2 gene results in the transparent integuments of Ectropis grisescens larvae

文献类型： 外文期刊

作者： Jia Li Li;Xiang Lin Zhuang;Ting Ting Yuan;Xiao Ming Cai;Zong Xiu Luo;Lei Bian;Zong Mao Chen;Zhao Qun Li;Nai Yong Liu

作者机构：

关键词： BLOS2;CRISPR/Cas9;Ectropis grisescens;Genome editing;Pigmentation

期刊名称： Journal of Asia-Pacific Entomology

ISSN： 1226-8615

年卷期： 2022 年 25 卷 1 期

页码：

收录情况： SCIE（2022版）

摘要： The body colors of insects have evolved specialized roles in body protection, signaling and physiology. In some case, the larvae can camouflage their body colors to adapt the habitants and protect themselves. However, the genetic and molecular basis on larval body colors of the tea geometrid, Ectropis grisescens, remains poorly known. Here, we reported an effect of the lysosome-related organelles complex-1, subunit 2 (EgriBLOS2) gene knockout on larval integuments of E. grisescens, by using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Combining transcriptomic data and PCR approaches, we first identified the EgriBLOS2 gene from E. grisescens, which encoded 137 amino acids and comprised 3 introns. In the phylogenetic analysis, EgriBLOS2 clustered in the Lepidoptera clade with high conservation to members in other lepidopteran species. Developmental expression profiles revealed that EgriBLOS2 was constantly transcribed at each stage, in which its expression was significantly lower in 2nd instar larvae than that of other instars. After injecting EgriBLOS2-specific guide RNA and Cas9 messenger RNA into eggs, 70% of larvae showed the translucent integuments in G0 generation, with an emphasis on black splayed patterns in the 2nd and 8th segments of abdomens. However, some typical characteristics of larvae were not obviously changed in mutant instars, such as ocelli, mouthparts and other appendants. This study has unraveled the roles of EgriBLOS2 in the formation of larval integument colors, and provides an alternative strategy for pest management based on the colors in this species.

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