数字农科院2.0

Development of certified reference materials for two acylated anthocyanins

文献类型： 外文期刊

作者： Guowen Li;Peiqing Yang;Xue Wang;Mengrui Yang;Tongtong Wang;Shini Yang;Xiaojun Liao;Zhenzhen Xu

作者机构：

关键词： Acylation;Characterization;Mass balance method;Quantitative nuclear magnetic resonance;Red cabbage;Uncertainty

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2025 年 492 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The development of anthocyanin certified reference materials (CRMs) is critical for accurate anthocyanin determination. In this work, two anthocyanin CRMs – cyanidin 3-O-(2-O-glucosyl, 6-O-sinapoyl)glucoside, 5-O-glucoside trifluoroacetate (Cy3(sin)diG5G trifluoroacetate) and cyanidin 3-O-(2-O-glucosyl, 6-O-p-coumaroyl)glucoside, 5-O-glucoside trifluoroacetate (Cy3(p-cum)diG5G trifluoroacetate) - were developed. Their purities were determined using a combination of mass balance (MB) and quantitative nuclear magnetic resonance (qNMR) methods. The determined purity values were 92.9 % and 94.7 %, with expanded uncertainties of 0.4 % and 0.3 % (k = 2), respectively. The obtained CRMs exhibited homogeneity and remained stable during both short-term and long-term storage at 4 °C and −20 °C. Furthermore, the prepared CRMs were successfully applied to the analysis of real samples. This work developed two anthocyanin CRMs that can be used for quality control and product traceability of natural anthocyanin products. It also provided a feasible guideline for the further development of anthocyanin CRMs.

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