数字农科院2.0

Enhanced high-solids enzymatic hydrolysis of rice straw by intermittent ball milling

文献类型： 外文期刊

作者： Guanya Ji;Bo Zhang;Bo Feng;Yuchen Xing;Bo Tao;Qijian Niu;Jinxiu Song;Zhong Tang;Haiyan Zhang

作者机构：

关键词： Adsorption mechanism;Enzymatic hydrolysis;High-solids loading;Intermittent ball milling;Rice straw

期刊名称： Biomass and Bioenergy

ISSN： 1873-2909

年卷期： 2025 年 204 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The “high-solids effect” of enzymatic hydrolysis systems at high dry matter presents a significant challenge, limiting the efficiency of biomass conversion. Herein, we explore the potential of intermittent ball milling as a strategy to overcome this hurdle, demonstrating its efficacy in enhancing reactivity, accelerating reaction kinetics, and reducing waste. The results revealed that this method increases glucose yield of rice straw at high-solid loading of 25 % (w/v) by 91.5 % after 12 h, compared to traditional enzymatic hydrolysis. Intermittent ball milling reduces particle size from 220.7 μm to 14.4 μm, enhancing substrate surface area and cellulose accessibility. We observed that cellulase adsorption and transfer in the high-solids enzymatic hydrolysis systems were most effective within the first 5 min of each milling cycle, leading to a 2.9-fold increase in adsorption after 12 h. These findings highlight the potential of intermittent ball milling to enhance high-solids enzymatic hydrolysis efficiency for biomass.

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