数字农科院2.0

Development and application of a qPCR assay for Lawsonia intracellularis in Tibetan pigs

文献类型: 外文期刊

作者: 肖晨东;;王艳琼;;薛丽虹;;赵红艳;;夏长友;;赵小刚

关键词: Tibetan pig; Lawsonia endocellularis; aspA; quantitative PCR; epidemiology

期刊名称: Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988

年卷期: 2025 年

页码:

收录情况: SCIE(2025版)

摘要: This study developed a rapid, sensitive, and specific TaqMan MGB based qPCR assay for detecting Lawsonia intracellularis in Tibetan pigs by targeting the aspA gene. The method showed no cross-reactivity with other common porcine enteric pathogens, and exhibited high sensitivity (detection limit: 7.056 copies/ μL) and excellent repeatability (CV < 2%). A standard curve with good linearity (R2 = 0.998) and 110.95% amplification efficiency was established. The assay was applied to 237 fecal samples collected from Tibetan pigs across eight townships in Xiangcheng County, Sichuan Province, in parallel with a national standard method. Results indicated a 19% overall positivity rate (45/237), with regional rates ranging from 11.11% to 26.67%. The established qPCR provides a reliable tool for field surveillance, and findings underscore the need to integrate L. intracellularis monitoring into local prevention systems to support healthy Tibetan pig production.

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