数字农科院2.0

Functional epitope mapping of porcine reproductive and respiratory syndrome virus nsp7α and nsp7β using monoclonal antibodies and development of a feasible ELISA

文献类型： 外文期刊

作者： Song Han;Hongyong Duan;Nan Yang;Shumao Wang;Fei Gao;Yifeng Jiang;Chaozhi Tang;Yanjun Zhou;Guangzhi Tong;Kuan Zhao;Liwei Li

作者机构：

关键词： Antigenic epitope;Enzyme-linked immunosorbent assay;Nonstructural protein 7

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 322 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) poses a significant threat owing to its considerable economic impact on the global swine industry. Nonstructural protein 7 (nsp7) plays an essential role in inducing the humoral immune response, resulting in the production of high and persistent antibodies in PRRSV-infected pigs. Therefore, nsp7 is considered the most appropriate marker for diagnostic development. Herein, we successfully expressed nsp7 using a prokaryotic expression system and generated eleven monoclonal antibodies. All antibodies exhibited specific reactivity and recognized the linear B-cell epitopes. By epitope mapping and antigenicity analysis, one novel epitope of nsp7α (141IPLPPKVLE149) and three novel epitopes of nsp7β (152PNAWGDEDRL161, 184KFWDKNSGD192, 211GDPVDFDPET220) were further identified. These epitopes were conserved and located on the surface of the nsp7 spatial structure. PRRSV-infected pigs exhibited strong antibody responses specific for the identified epitopes. The enzyme-linked immunosorbent assay (ELISA) developed using two epitope peptides of nsp7α and nsp7β showed high sensitivity, good specificity, and a 94.0 % coincidence rate with the commercial kit. The antibody was detected in pig sera using ELISA at 14 days post-infection, indicating its potential for PRRSV monitoring. The collective findings proved to be effective for detection and have promising application prospects for epidemiological surveillance and vaccine research.

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