数字农科院2.0

Expression profiling, functional characterization, and potential functional variants of the COL7A1 gene in sheep horn development

文献类型： 外文期刊

作者： Zhang, Shiwen;Li, Hao;Meng, Zhu;Liu, Fei;Hu, Yangshuo;Yang, Huaiqiang;Mei, Qiao;You, Xiangbin;Wang, Qiankun;Pan, Zhangyuan;Yang, Youbing

作者机构：

关键词： Sheep horn;SNPs;Three-dimensional structure

期刊名称： GENOMICS

ISSN： 0888-7543

年卷期： 2025 年 117 卷 6 期

页码：

收录情况： SCIE（2025版）

摘要： The morphological diversity of sheep horns affects livestock production, yet its molecular regulation is poorly understood. COL7A1, essential for anchoring the epidermis to the dermis, was investigated using RNA-seq, WGS, and protein interaction prediction. COL7A1 was highly expressed in soft horn and skin, with higher levels in Scurred than Horned sheep (p < 0.05), suggesting a role in horn morphology. Cross-species alignment of 18 COL7A1 sequences revealed 15 amino acids unique to horned ruminants. RNA-seq and WGS identified four allele-specific expression (ASE) loci and 18 SNPs linked to horn traits. Two missense variants-SNP8 (p. Glu181Asp) and SNP14 (p.Ala2857Thr)-may alter protein structure, with SNP14 showing ASE. Kompetitive Allele-Specific PCR (KASP) genotyping of SNP4 and SNP8 confirmed associations with horn length (p < 0.05). Protein interaction modeling indicated COL7A1 synergizes with laminin gamma 2 (LAMC2), implicating basement membrane assembly. These results provided molecular insights into ovine horn development and support marker-assisted selection.

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