数字农科院2.0

Analysis of Mitochondrial Sequence Deletion in the atp95′UTR Region and Design of Molecular Markers in Cotton(Gossypium spp.)

文献类型： 外文期刊

作者： Wanlin Kang; Lanlan Zhang; Zhiyu Li; Yuzhu Fang; Jinglong Yang; Yan Song; Shining Liu; Xiao Zhang; Rui Zhang

关键词： 5′UTR | atp9 gene | Gossypium | InDel | molecular markers | mtDN

期刊名称： Plant Breeding

ISSN： 0179-9541

年卷期： 2025 年

页码：

收录情况： SCIE（2024版）

摘要： Cotton (Gossypium) is the most important fibre crop in the world, consisting of 45 diploid and 7 tetraploid cotton species. These cotton varieties are valuable for breeding, but their molecular identification methods still need further study. We identified an insert/deletion site (AATTT) at the atp9 5′UTR region in the mitochondrial genome of cotton, which could be used to distinguish different cotton species. In this study, the target fragments of 33 cotton species (29 diploid and 4 tetraploid species) were amplified by PCR, and the PCR products potentially containing an EcoR I restriction site were subsequently digested and analysed. The sequencing results revealed that 27 out of 33 cotton species lacked ‘AATTT’ sequences, while six cotton species (G. longicalyx, G. hirsutum, G. barbadense, G. tomentosum, G. mustillinum and G. darwinii) were found to possess the sequences. Additionally, 39 SNPs were found in this region, and specific molecular markers for G. stocksii, G. bickii and E-genome were developed, respectively. The comparative analysis of mitochondrial sequences from diploid and tetraploid cotton species elucidated their genetic diversity and evolutionary relationships, and species-specific markers were able to discriminate among these species, thereby provided a foundation for more targeted use of wild genetic resources in cotton improvement and efforts to ensure their conservation.

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