Synergistic Insecticidal Activity Against Hyphantria cunea by Cry9Aa3 Mutants and Cry1Ah Combinations
文献类型: 外文期刊
作者: Xu, Pengdan;Wang, Zeyu;Zhang, Ying;Han, Jiaxing;Shu, Changlong;Liao, Min;Zhang, Jie;Geng, Lili
作者机构:
关键词: Hyphantria cunea;Bacillus thuringiensis;cry9-type genes;mutant;synergistic effect
期刊名称: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN: 1661-6596
年卷期: 2025 年 26 卷 8 期
页码:
收录情况: SCIE(2025版)
摘要: The larvae of Hyphantria cunea feed on plant leaves, causing significant losses to forestry and agricultural production. At present, cry1 genes such as cry1Ac and cry1Ah are mainly used to control H. cunea. To delay the problem of pest resistance induced by a single insecticidal gene, it is crucial to discover and develop new insecticidal genes or gene combinations. This study found cry9Aa3 and cry9Aa4 showed insecticidal activity against H. cunea. The toxicity of 14 mutants of Cry9Aa3 was analyzed and the LC50 of the triple-amino-acid substitution mutant 316LRG318AAA was 3.69 mu g/g, which represents a 1.49-fold increase in insecticidal activity compared to Cry9Aa3. Additionally, enhanced stability of this mutant was detected in the midgut juice of H. cunea. Cry9Aa3 and 316LRG318AAA, in combination with Cry1Ah, demonstrated synergistic effects against H. cunea, with synergistic factors of 4.76 and 8.33, respectively. This study has identified the mutant 316LRG318AAA and its combination with Cry1Ah as exhibiting high toxicity against H. cunea, providing valuable genetic resources for the development of transgenic poplars and holding significant importance for delaying resistance in this pest.
分类号:
- 相关文献
作者其他论文 更多>>
-
Synergistic Tuning of Conformational Dynamics, Electron Tunneling, and Substrate Positioning Enhances Electron Transfer in a P450 Chimera for Calcifediol Biosynthesis
作者:Liang, Ziqi;Song, Xitong;Cheng, Shuming;Shen, Yiwen;Zhang, Jie;Wang, Yaru;Luo, Huiying;Yao, Bin;Wang, Binju;Tu, Tao
关键词:calcifediol biocatalysis;conformational;dynamics modulation;electron transfer;multi-domain P450s engineering
-
Genomic and phenotypic analysis of Bacillus strains from Protaetia brevitarsis frass reveals plant growth promoting potential and biocontrol activity against Fusarium wheat crown rot
作者:Zhao, Shuan;Wang, Hongyu;Guo, Tongtong;Hu, Xiaolong;Cao, Beibei;Zhang, Jie;Shu, Changlong;Wang, Kui
关键词:Protaetia brevitarsis frass;Bacillus;plant growth promoting;biocontrol;Fusarium wheat crown rot;genomic analysis;bioinoculants
-
Lipid Remodeling Underlies Salt Tolerance in Carex rigescens: A Comparative Lipidomic Analysis
作者:Zhang, Jie;Wu, Yiming;Ma, Chengze;Shi, Yi;Li, Mingna;Hu, Qiannan
关键词:Phospholipids;Glycolipids;Salt stress;Turfgrass;Carex rigescens
-
Structure insight into FtsZ function maintaining under acid stress of Streptococcus mutans
作者:Chen, Yuxing;Li, Yongliang;Niu, Jiahao;Yang, Liuchang;Chi, Yaqi;Cai, Xue;Xin, Fengjiao;Zhang, Jie;Fang, Xianyang;Gao, Yiqin;Mondal, Manas;Wang, Xiaoyan
关键词:
-
Characteristics of rhizosphere and bulk soil microbial communities in pear trees
作者:Geng, Jianxun;Chen, Liang;Yang, Xiaomin;Geng, Lili;Duan, Jiangyan;Wang, Meiling
关键词:Pear tree;Rhizobacteria;16S rRNA gene sequencing;Antagonistic bacteria;Ecological adaptation
-
Rice RuBisCO activase promotes the dark-induced leaf senescence by enhancing the degradation of filamentation temperature-sensitive H
作者:Zhang, Yanli;Dong, Guojun;Wu, Xiaoyue;Chen, Fei;Ruan, Banpu;Jiang, Yaohuang;Zhang, Ying;Liu, Lu;Yuan, Yao-Wu;Wu, Limin;Wei, Jian;Qian, Qian;Yu, Yanchun
关键词:rice (Oryza sativa L.);RuBisCO activase (RCA);dark-induced senescence (DIS);filamentation temperature-sensitive H (OsFtsH1);chloroplast
-
Methyl eugenol regulates mating behavior in oriental fruit flies by enhancing lek attractiveness
作者:Zhang, Jie;Liu, Wei;Chang, Hetan;Wang, Qi;Yuan, Jinxi;Liu, Leyuan;Liu, Chenhao;Zhang, Yan;Ru, Chuanjian;Yan, Shanchun;Hansson, Bill S.;Wang, Guirong
关键词:plant volatile compounds;oriental fruit fly;methyl eugenol;olfactory receptor