数字农科院2.0

Transcriptome Profiling Reveals the Gene Network Responding to Low Nitrogen Stress in Wheat

文献类型： 外文期刊

作者： Yiwei Wang;Pengfeng Li;Yiwang Zhu;Yuping Shang;Zhiqiang Wu;Yongfu Tao;Hongru Wang;Dongxi Li;Cuijun Zhang

作者机构：

关键词： （1-1-2）low nitrogen;nitrogen signaling network;RNA-seq;wheat

期刊名称： Plants

ISSN： 2223-7747

年卷期： 2024 年 13 卷 3 期

页码：

收录情况： SCIE（2024版）

摘要： As one of the essential nutrients for plants, nitrogen (N) has a major impact on the yield and quality of wheat worldwide. Due to chemical fertilizer pollution, it has become increasingly important to improve crop yield by increasing N use efficiency (NUE). Therefore, understanding the response mechanisms to low N (LN) stress is essential for the regulation of NUE in wheat. In this study, LN stress significantly accelerated wheat root growth, but inhibited shoot growth. Further transcriptome analysis showed that 8468 differentially expressed genes (DEGs) responded to LN stress. The roots and shoots displayed opposite response patterns, of which the majority of DEGs in roots were up-regulated (66.15%; 2955/4467), but the majority of DEGs in shoots were down-regulated (71.62%; 3274/4565). GO and KEGG analyses showed that nitrate reductase activity, nitrate assimilation, and N metabolism were significantly enriched in both the roots and shoots. Transcription factor (TF) and protein kinase analysis showed that genes such as MYB-related (38/38 genes) may function in a tissue-specific manner to respond to LN stress. Moreover, 20 out of 107 N signaling homologous genes were differentially expressed in wheat. A total of 47 transcriptome datasets were used for weighted gene co-expression network analysis (17,840 genes), and five TFs were identified as the potential hub regulatory genes involved in the response to LN stress in wheat. Our findings provide insight into the functional mechanisms in response to LN stress and five candidate regulatory genes in wheat. These results will provide a basis for further research on promoting NUE in wheat.

分类号：

  • 相关文献

[1]充分发掘保护利用生物多样性;促进农业可持续发展——《生物多样性与小麦改良》简评. 李祥洲. 1997

[2]Rna-Seq Analysis Provides The First I.nsights Into The Phylogenetic R elationship And Interspecific Variation Between Agropyron Cristatum And Wheat. Zhou, SH,Yan, BQ,Li, F,Zhang, J,Zhang, J,Ma, HH,Liu, WH,Lu, YQ,Yang, XM,Li, XQ,Liu, X,Li, LH. 2017

[3]Transcriptome Analysis of Roots from Wheat (Triticum aestivum L.) Varieties in Response to Drought Stress. Xi, Wei,Hao, Chenyang,Li, Tian,Wang, Huajun,Zhang, Xueyong. 2023

[4]A Multi-Omics Approach for Rapid Identification of Large Genomic Lesions at the Wheat Dense Spike (wds) Locus. Zhenyu Wang,Shu Tao,Shaoshuai Liu,Meiling Jia,Dada Cui,Guoliang Sun,Zhongyin Deng,Fang Wang,Xingchen Kong,Mingxue Fu,Yuqing Che,Ruyi Liao,Tao Li,Shuaifeng Geng,Long Mao,Aili Li. 2022

[5]Transcriptome Dynamics in Triticum aestivum Genotypes Associated with Resistance against the Wheat Dwarf Virus. Abdoallah Sharaf,Przemysław Nuc,Jan Ripl,Glenda Alquicer,Emad Ibrahim,Xifeng Wang,Midatharahally N. Maruthi,Jiban Kumar Kundu. 2023

[6]Root system architecture of historical spring wheat cultivars is associated with alleles and transcripts of major functional genes. Saman Maqbool,Suhaib Ahmad,Zarnishal Kainat,Muhammad Ibrar Khan,Ammarah Maqbool,Muhammad Adeel Hassan,Awais Rasheed,Zhonghu He. 2022

[7]Genome-wide association study for phosphate responsive root hair length and density in bread wheat. Saman Maqbool,Fatima Saeed,Ammarah Maqbool,Muhammad Ibrar Khan,Mohsin Ali,Awais Rasheed,Xianchun Xia,Zhonghu He. 2023

[8]30个小麦新品系抗条锈病基因分析及成株期抗病性评价. 冯晶,蔺瑞明,林凤,徐世昌. 2013

[9]小麦抗菌蛋白编码基因BS108的克隆及其转基因抗病小麦的创制. 李钊,周淼平,张增艳,庄洪涛,徐惠君,杜丽璞,叶兴国. 2010

[10]渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文). 孙朝霞,侯思宇,王玉国. 2008

[11]普通小麦品种辐射敏感性模糊聚类分析<英文>. 冯志杰,王琳清. 1992

[12]基因枪转化小麦主要轰击参数的优化. 闵东红,何莎,张彦,夏兰琴. 2013

[13]小麦矮缩病毒分子群体遗传结构研究. 刘艳,王锡锋,周广和. 2008

[14]河流型水牛TGF-β基因家族鉴定及其胚胎发育早期的表达分析. 庞春英,梁莎莎,马小娅,陆杏蓉,段安琴,邓廷贤,黄韵琪,梁贤威. 2020

[15]拟南芥ago1-27突变体的RNA-seq分析. 马轩,李盛本,莫蓓莘,曹晓风,陈雪梅. 2017

[16]本生烟响应蛋白激发子PevD1的差异表达基因鉴定与分析. 梁颖博,李泽,邱德文,曾洪梅,李广悦,杨秀芬. 2019

[17]基于RNA-Seq的甘蔗主茎和分蘖茎转录组建立及初步分析. 丘立杭,罗含敏,陈荣发,黄杏,陈忠良,范业赓,陈栋,李杨瑞,吴建明. 2018

[18]基于RNA-Seq技术的o2胚乳差异表达分析. 朱慧,周志强,张东民,周昱婕,张晓星,刘红军,宋丽雅,李明顺. 2019

[19]雏鸡红细胞免疫相关基因差异表达的时序性研究. 贾悦,李月健,喻进,张晨亮,李娜娜,卢晓晓,张宁,李欣,张鼎,郝卫芳,马海利,高文伟,赵宇军,高诗敏,李建慧,李桂兰,闫芳,韩凌霞,宁官保,田文霞. 2015

[20]志贺菌耐药性与非编码RNA的关系研究. 刘翠翠,魏小娟,周绪正,李冰,牛建荣,王婧,朱阵,张继瑜. 2014

作者其他论文 更多>>