数字农科院2.0

Creating artificial miR2118a/b to boost yield and broad-spectrum resistance in soybean via CRISPR/Cas9-targeted mutation

文献类型： 外文期刊

作者： Limiao Chen; Wenqi Ouyang; Yanfeng Hu; Lihua Peng; Peike Chen; Wei Guo; Hongli Yang; Juan Xu; Mengyue Pan; Dami Xu; Xinyang Wang; Chanjuan Zhang; Shuilian Chen; Qingnan Hao; Songli Yuan; Yi Huang; Zhihui Shan; Zhonglu Yang; Rui Xia; Tarek Hewezi;;Haifeng Chen; Lam-Son Phan Tran; Xinan Zhou; Dong Cao

关键词： Soybean; miR2118; miR2118-5p; broad-spectrum resistance; yield; phasiRNA; artificial miRNAs

期刊名称： TRENDS IN BIOTECHNOLOGY

ISSN： 0167-7799

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： While regulatory functions of mature miRNAs are well established, the functions of miRNAs* and their potential for genetic engineering in crop improvement remain underexplored. Here, we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) to generate artificial miR2118a/b (amiR2118a/b) by editing miR2118a/b-5p and obtained several amir2118a/b mutants in soybean (Glycine max). miR2118a/b-5p modifications altered the secondary structure of precursor amiR2118a/b (pre-amiR2118a/b) and reduced mature miR2118a/b levels. These amir2118a/b mutants retained the ability to initiate biogenesis of phased small interfering RNAs (phasiRNAs), albeit with a reduced abundance compared with wild-type (WT) plants. Furthermore, these mutants upregulated the expression of genes related to growth and defense under normal and Pseudomonas syringae pv. glycinea (Psg)-infected conditions, respectively. Notably, two transgene-free amir2118 mutants exhibited enhanced resistance to Psg, soybean cyst nematode (SCN), and root-knot nematode (RKN), and achieved increased yield under pathogen-free field conditions. This study provides a strategy to generate artificial miRNAs (amiRNAs) for crop improvement through the CRISPR/Cas system by mutating miRNAs* in crops.

分类号：

  • 相关文献

[1]Synthetic Hexaploid Wheat: Yesterday, Today, A.nd Tomorrow. Li, AL, Liu, DC, Yang, WY, Kishii, M, Mao, L. 2018

[2]Small- and medium-sized seeds are more conducive to achieving high soybean yield due to the outstanding seedling survival and yield formation characteristics. 华建鑫,,,,朱玉鹏,,李瑞东,,,,吴宗声,,,,徐一帆,,,,孙石,,,,徐彩龙,,,,宋雯雯,,吴存祥. 2025

[3]Small noncoding RNA discovery and profiling with sRNAtools based on high-throughput sequencing. Qi Liu,Changjun Ding,Xiaoqiang Lang,Ganggang Guo,Jiafei Chen,Xiaohua Su. 2021

[4]Dissecting The Genetic Basis Of F.iber Quality And Yield T raits In Interspecific Backcross Populations Of Gossypium Hirsutum X Gossypium Barbadense. Lu, Quanwei,Gong, Juwu,Shi, Yuzhen,Jamshed, Muhammad,Zhang, Baocai,Zhang, Jinfa,Ge, Qun,Li, Shaoqi,Xiang, Xianghui,Yuan, Youlu,Gong, Wankui,Shang, Haihong,Li, Junwen,Pan, Jingtao,Deng, Xiaoying,Liu, Aiying. 2019

[5]Effects Of Planting Patterns On Y.ield, Quality, And Defoliation I n Machine-Harvested Cotton. Wang Fang:yong,Kong Xian:hui,Yu Yu,Han Huan:yong,Liu Jing:de,Lin Hai,Wang Xu:wen,Chen Bing,Ning Xin:zhu. 2019

[6]A Model System For Off S.eason Cherry Production In N orthern China. Zhang, Q. J.,Zhou, Z. H.,Yu, K. H.,Gu, D. J.. 2018

[7]Light Shading Improves The Yield A.nd Quality Of Seed I n Oil-Seed Peony (Paeonia Ostii Feng Dan). Dong He-zhong,Han Chen-jing,Hao Jian-mei,Song Hua-dong,Wang Qi,Wang Shou-hai,Wang Shou-hai,Zhang Hong-bao. 2018

[8]Advances In Research Of Lodging A.nd Evaluation In Sugarcane. Li, Y. R.,Li, Y. J.,Li, X.,Li, Y. R.,Yang, L. T.,Lin, S. H.,Liang, Q.,Huang, Q. Y.,Yang, L. T.,Li, X.. 2019

[9]Optimization Of Ultrasonic-Microwave Assisted Extraction O.f Oligosaccharides From Lotus ( Nelumbo Nucifera Gaertn.) Seeds. Lu, Xu,Miao, Song,Zheng, Baodong,Lu, Xu,Lu, Xu,Cao, Ran,Li, Huang,Lu, Xu,Zheng, Zhichang,Zheng, Yafeng,Zheng, Yafeng,Zheng, Baodong,Xiao, Jianbo,Zheng, Baodong,Zheng, Baodong,Yu, Hua. 2017

[10]Adaptability And Stability Comparisons Of I.nbred And Hybrid Cotton I n Yield And Fiber Quality Traits. Iqbal, Muhammad Shahid,Qi, Tingxiang,Zhang, Xuexian,Guo, Liping,Wu, Jianyong,Tang, Huini,Shahzad, Kashif,Wang, Hailin,Zhang, Bingbing,Feng, Juanjuan,Zhang, Meng,Qiao, Xiuqin,Wu, Jianyong,Xing, Chaozhu,Iqbal, Muhammad Shahid. 2019

[11]Research Progress On Heat Stress O.f Rice At Flowering S tage. Wang Yaliang,Zhu Defeng,Zhou Jianxia,Zhang Yikai,Zeng Yongjun,Zhang Yuping,Shi Qinghua,Wang Yaliang,Wang Lei,Xiang Jing,Hu Shengbo,Chen Huizhe. 2019

[12]Identification And Qtl Mapping Of Z.550, A Rice Backcrossed I nbred Line With Increased Grains Per Panicle. Yang Zheng-lin,Wang Shi-ming,Cui Guo-qing,Ling Ying-hua,Ma Fu-ying,Xia Sai-sai,Zhang Chang-wei,He Guang-hua,Li Yun-feng,Wang Hui,Zhao Fang-ming. 2019

[13]Using Yield Quantitative Trait Locus T.argeted Ssr Markers To S tudy The Relationship Between Genetic Distance And Yield Heterosis In Upland Cotton (Gossypium Hirsutum). Shahzad, Kashif,Qiao, Xiuqin,Qi, Tingxiang,Wu, Jianyong,Xing, Chaozhu,Wang, Hailin,Zhang, Xuexian,Li, Xue,Zhang, Jinfa,Tang, Huini,Guo, Liping. 2019

[14]Mutation Of The Chloroplast-Localized Phosphate Transporter Ospht2;1 Reduces Flavonoid Accumulation And Uv Tolerance In Rice. Sun, Shu-Bin,Wang, Lan,Ai, Hao,Hu, Zhi,Shen, Xing,Lin, Hong-Hui,Yan, Yan,Xu, Guo-Hua,Yang, Xiao-Li,Yang, Jian,Liu, Xiu-Li,Wang, Lan,Xie, Meng-Yang,Wang, Xiao-Wen. 2020

[15]Photosynthetic And Physiological Responses Of F.oxtail Millet (Setaria Italica L .) To Low-Light Stress During Grain-Filling Stage. Wen, Y. Y.,Wang, H. F.,Yuan, X. Y.,Zhang, L. G.,Yuan, X. Y.,Guo, P. Y.,Dong, S. Q.,Huang, L.,Song, X. E.,Qi, X.. 2017

[16]Association Of Yield-Related Traits In F.ounder Genotypes And Derivatives O f Common Wheat (Triticum Aestivum L.). Shi, Weiping,Cheng, Jingye,Zhang, Zheng,Yu, Jin,Li, Xinlei,Sun, Daizhen,Guo, Jie,Guo, Pingyi,Hao, Chenyang. 2018

[17]Growth Dynamics Of Maize (Zea M.ays L.) And Weeds I n Response To Different Nitrogen Levels And Weeding Intervals. Wei, Shouhui,Saeed, Muhammad,Huang, Zhaofeng,Huang, Hongjuan,Khattak, Mansoor Khan,Saeed, Muhammad,Zhang, Chaoxian,Alf, Murad. 2018

[18]Synthetic Hexaploid Wheat: Yesterday, Today, A.nd Tomorrow. Kishii, Masahiro,Li, Aili,Liu, Dengcai,Mao, Long,Yang, Wuyun. 2018

[19]Plant Density Influences Reproductive Growth, Lint Yield And Boll Spatial Distribution Of Cotton. Wang, Guoping,Wang, Zhanbiao,Khan, Nangial,Feng, Lu,Li, Xiaofei,Yang, Beifang,Han, Yingchun,Feng, Lu,Xing, Fangfang,Xiong, Shiwu,Li, Yabing,Fan, Zhengyi,Lei, Yaping,Wang, Zhanbiao,Li, Yabing. 2020

[20]The Effects Of Rootstocks On Performances Of Three Late-Ripening Navel Orange Varieties. Zhu Shi-ping,Zhu Shi-ping,Zhao Xiao-chun,Zhao Xiao-chun,Yu Xin,Hong Qi-bin,Hong Qi-bin,Yu Xin,Gong Gui-zhi,Huang Tao-jiang,Gong Gui-zhi,Zeng An-zhong,Xiang Jin-song. 2020

作者其他论文 更多>>