数字农科院2.0

The Infection of the Japanese Encephalitis Virus SA14-14-2 Strain Induces Lethal Peripheral Inflammatory Responses in IFNAR Deficiency Mice

文献类型： 外文期刊

作者： Liu, Juan;Jing, Wenxian;Fang, Yongxiang;He, Xiaobing;Chen, Guohua;Jia, Huaijie;Wang, Jingyu;Jing, Zhizhong

作者机构：

关键词： Japanese encephalitis virus;SA14-14-2 strain;IFNAR deficiency mice;infection;immunopathogenesis;peripheral inflammation

期刊名称： FRONTIERS IN MICROBIOLOGY

ISSN：

年卷期： 2022 年 12 卷

页码：

收录情况： JCR（2021版）

摘要： The Japanese encephalitis virus (JEV) is a leading cause of mosquito-borne viral encephalitis worldwide. Clinical symptoms other than encephalitis, on the other hand, are substantially more prevalent with JEV infection, demonstrating the relevance of peripheral pathophysiology. We studied the peripheral immunopathogenesis of JEV using IFNAR deficient (IFNAR(-/-)) mice infected with the SA14-14-2 strain under the BSL-2. The body weight and survival rate of infected-IFNAR(-/-)mice decreased significantly. Infected-IFNAR(-/-)mice's liver and spleen demonstrated obvious tissue damage and inflammatory cell infiltration. There was also extensive viral replication in the organs. IFN-alpha/beta protein expression was dramatically elevated in peripheral tissues and serum, although the related interferon-stimulated genes (ISGs) remained low in the spleen and liver of infected-IFNAR(-/-)animals. Consistently, the differentially expressed genes (DEGs) analysis using RNA-sequencing of spleens showed inflammatory cytokines upregulation, such as IL-6, TNF-alpha, and MCP-1, and IFN-gamma associated cytokine storm. The infiltration of macrophages and neutrophils in the spleen and liver of SA14-14-2-infected IFNAR(-/-) mice was dramatically elevated. However, there was no significant difference in tissue damage, viral multiplication, or the production of IFN alpha/beta and inflammatory cytokines in the brain. Infection with the JEV SA14-14-2 strain resulted in a lethal peripheral inflammatory response and organ damage without encephalitis in IFNAR(-/-) mice. Our findings may help shed light on the peripheral immunopathogenesis associated with clinical JEV infection and aid in developing treatment options.

分类号：

  • 相关文献

[1]A simple method for developing an infectious cDNA clone of Japanese encephalitis virus. Zheng, Hao,Zheng, Xuchen,Tong, Wu,Liu, Fei,Liang, Chao,Wang, Tao,Gao, Fei,Li, Liwei,Shan, Tongling,Li, Guoxin,Tong, Guangzhi,Zheng, Hao,Tong, Wu,Tong, Guangzhi.

[2]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

[3]Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus. Tong, Guang-Zhi,Bin Wang,Hua, Rong-Hong,Tian, Zhi-Jun,Chen, Na-Sha,Zhao, Fu-Rong,Liu, Tian-Qiang,Wang, Yun-Feng,Tong, Guang-Zhi. 2009

[4]Nitazoxanide inhibits the replication of Japanese encephalitis virus in cultured cells and in a mouse model. Shi, Zixue,Wei, Jianchao,Deng, Xufang,Qiu, Yafeng,Shao, Donghua,Li, Beibei,Zhang, Keyu,Xue, Feiqun,Ma, Zhiyong,Li, Shuqing,Wang, Xiaodu. 2014

[5]Antigenic epitopes mapping and functional analysis of domain III of Japanese encephalitis virus envelope protein. Yan Li-Ping,Hua Rong-Hong,Tong Guang-Zhi,Yan Li-Ping,Qi Wen-Bao. 2008

[6]Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese encephalitis virus for a subunit vaccine candidate. Hua, Rong-Hong,Li, Ye-Nan,Chen, Zhen-Shi,Liu, Li-Ke,Huo, Hong,Wang, Xiao-Lei,Guo, Li-Ping,Shen, Nan,Wang, Jing-Fei,Bu, Zhi-Gao. 2014

[7]Antibodies generated by immunization with the NS1 protein of West Nile virus confer partial protection against lethal Japanese encephalitis virus challenge. Sun, EnCheng,Zhao, Jing,TaoYang,Xu, QingYuan,Qin, YongLi,Wang, WenShi,Wei, Peng,Wu, DongLai. 2013

[8]Genetic instability of Japanese encephalitis virus cDNA clones propagated in Escherichia coli. Zheng, Xuchen,Tong, Wu,Liu, Fei,Liang, Chao,Gao, Fei,Li, Guoxin,Tong, Guangzhi,Zheng, Hao,Tong, Guangzhi,Zheng, Hao.

[9]Porcine alpha interferon inhibit Japanese encephalitis virus replication by different ISGs in vitro. Liao, Xuewen,Yao, Huijuan,Fan, Shengchao,Miao, Denian,Liu, Ke,Zhou, Bin,Chen, Puyan,Liu, Ke. 2013

[10]Molecular characterization of Japanese encephalitis virus strains prevalent in Chinese swine herds. Zheng, Hao,Shan, Tongling,Deng, Yu,Sun, Chunqing,Yuan, Shishan,Yin, Yang,Tong, Guangzhi. 2013

[11]mosGCTL-7, a C-Type Lectin Protein, Mediates Japanese Encephalitis Virus Infection in Mosquitoes. Liu, Ke,Qian, Yingjuan,Jung, Yong-Sam,Zhou, Bin,Cao, Ruibing,Shen, Ting,Chen, Puyan,Zhu, Huaimin,Liu, Ke,Shao, Donghua,Wei, Jianchao,Ma, Zhiyong,Qiu, Yafeng.

[12]Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection. Ishag, Hassan Z. A.,Li, Chen,Wang, Fengjuan,Mao, Xiang,Mao, Xiang,Ishag, Hassan Z. A..

[13]A Simple Method For Developing A.n Infectious Cdna Clone O f Japanese Encephalitis Virus. Zheng, H, Zheng, XC, Tong, W, Liu, F, Liang, C, Wang, T, Gao, F, Li, LW, Shan, TL, Li, GX, Tong, GZ. 2017

[14]Possible Pathogenicity Of Japanese Encephalitis V.irus In Newly Hatched D omestic Ducklings. Xiao, CG, Wang, X, Cui, GH, Pang, LL, Xu, JP, Li, CX, Zhang, JJ, Liu, K, Li, BB, Shao, DH, Qiu, YF, Wei, JC, Ma, ZY. 2018

[15]Phenotypic And Genotypic Comparison Of A Live-Attenuated Genotype I Japanese Encephalitis Virus Sd12-F120 Strain With Its Virulent Parental Sd12 Strain. Anwar, MN, Wang, X, Hameed, M, Wahaab, A, Li, CX, Sharma, M, Pang, LL, Malik, MI, Liu, K, Li, BB, Qiu, YF, Wei, JC, Ma, ZY. 2020

[16]Novel reverse genetics of genotype I and III Japanese encephalitis viruses assembled through transformation associated recombination in yeast: The reporter viruses expressing a green fluorescent protein for the antiviral screening assay. Chenxi Li,Xuan Chen,Yanyang Zhou,Jingbo Hu,Xinjie Wang,Yanhua Li. 2022

[17]Experimental Infection Of Newly Hatched Domestic Ducklings Via Japanese Encephalitis Virus-Infected Mosquitoes. Di, D, Li, CX, Zhang, JJ, Hameed, M, Wang, X, Xia, QQ, Li, H, Xi, SM, Li, ZJ, Liu, K, Li, BB, Shao, DH, Qiu, YF, Wei, JC, Ma, ZY. 2020

[18]Virulence and Cross-Protection Conferred by an Attenuated Genotype I-Based Chimeric Japanese Encephalitis Virus Strain Harboring the E Protein of Genotype V in Mice. Xia, Qiqi,Zhang, Yan,Yang, Yang,Ma, Xiaochun,Guan, Zhixin,Zhang, Junjie,Li, Zongjie,Liu, Ke,Li, Beibei,Shao, Donghua,Qiu, Yafeng,Wei, Jianchao,Ma, Zhiyong. 2022

[19]Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection. Ma X.,Li C.,Xia Q.,Zhang Y.,Yang Y.,Wahaab A.,Liu K.,Li Z.,Li B.,Qiu Y.,Wei J.,Ma Z.. 2022

[20]Rapid Differential Detection of Japanese Encephalitis Virus and Getah Virus in Pigs or Mosquitos by a Duplex TaqMan Real-Time RT-PCR Assay. Zhang Y.,Li Y.,Guan Z.,Yang Y.,Zhang J.,Sun Q.,Li B.,Qiu Y.,Liu K.,Shao D.,Ma Z.,Wei J.,Li P.. 2022

作者其他论文 更多>>