数字农科院2.0

Engineering CO2-Fixing Carboxysome into Saccharomyces cerevisiae to Improve Ethanol Production

文献类型： 外文期刊

作者： Li, Mengqi;Zeng, Simin;Guo, Yunling;Ji, Jie;Fan, Qiuling;Duanmu, Deqiang

作者机构：

关键词： （0-1-3）bacterial microcompartment;alpha-carboxysome;carbon dioxide fixation;bioethanol production;Halothiobacillus neapolitanus;Saccharomyces cerevisiae

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1661-6596

年卷期： 2025 年 26 卷 19 期

页码：

收录情况： SCIE（2025版）

摘要： Bacterial microcompartments (BMCs) are intracellular structures for compartmentalizing specific metabolic pathways in bacteria. As a unique type of BMCs, carboxysomes utilize protein shells to sequester ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase for efficient carbon dioxide (CO2) fixation. This study aims to reconstruct an alpha-carboxysome in Saccharomyces cerevisiae and investigate its metabolic effects. Here, genes of the cso operon from Halothiobacillus neapolitanus, Calvin cycle-related enzyme phosphoribulokinase (PRK) from Spinacia oleracea, and two S. cerevisiae chaperone genes, HSP60 and HSP10, were introduced into S. cerevisiae. The engineered yeast strain demonstrated assembled and enzymatically active Rubisco, significant increase in ethanol production and reduction in the byproduct glycerol. Formation of the alpha-carboxysome structures was observed after purification by sucrose density gradient centrifugation. The engineered yeast strain harboring functional alpha-carboxysome has the potential for enhancing bioethanol production.

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