数字农科院2.0

Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus

文献类型： 外文期刊

作者： Sun, Yue;Zhang, Ru;Wang, Huiyu;Sun, Zheng;Yi, Le;Tu, Changchun;Yang, Yanling;He, Biao

作者机构：

关键词： Virome;Akabane disease virus;Arbovirus;Cattle;Reassortment

期刊名称： BMC VETERINARY RESEARCH

ISSN：

年卷期： 2024 年 20 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： BackgroundAt the end of 2021, an epidemic of reproductive abnormalities in cows occurred in Jilin Province, China, posing an urgent need for a rapid diagnosis.ResultsTo identify the cause of the disease, a total of 172 samples were collected from 21 dead calves and 45 aborting or pregnant cows in 10 farms across the province. Routine PCR or RT-PCR detection did not find any common abortion-related agents. We then employed the viromics-based precision diagnosis method to analyze these samples, and the read-based annotation showed signals of an Akabane disease virus (AKAV) in some libraries. To further identify the virus, nested RT-PCR detection revealed that 52.3% (11/21) of dead calves and 26.6% (12/45) of cows were positive for the virus. Phylogenetic analysis of the partial fragments showed that the S segment of the virus was 100% identical to the Chinese strain TJ2016, but its M and L segments shared 94.3% and 96.5% identities with an Israeli strain.ConclusionsThe viromic and molecular results suggested that these animals were infected with a reassortant AKAV. Coupled with the clinical signs, the virus should be responsible for the epizootic, highlighting that molecular and serological surveys of the virus in cows during early pregnancy, as well as ecological investigation in its arthropod vectors, are necessary.

分类号：

  • 相关文献

[1]A survey of fecal virome and bacterial community of the diarrhea-affected cattle in northeast China reveals novel disease-associated ecological risk factors. Zhu, Qinghe,Qi, Shanshan,Guo, Donghua,Li, Chunqiu,Su, Mingjun,Wang, Jianfa,Li, Zijian,Yang, Dan,Sun, Haibo,Wang, Xiaoran,Wang, Meijiao,Wu, Haoyang,Yu, Shiping,Bai, Wenfei,Zhang, Yongchen,Yang, Xu,Jiang, Limin,Liu, Jiaying,Zhao, Yingying,Xing, Xiaoxu,Shi, Da,Feng, Li,Sun, Dongbo. 2024

[2]Detection of arboviruses in Culicoides (Diptera: Ceratopogonidae) collected from animal farms in the border areas of Yunnan Province, China. Di DI,Chen xi LI,Zong jie LI,Xin WANG,Qi qi XIA,Mona SHARMA,Bei bei LI,Ke LIU,Dong hua SHAO,Ya feng QIU,W. A.I. Soe-Soe,Shi biao YANG,Jian chao WEI,Zhi yong MA. 2021

[3]Isolation and Characterization of Getah Virus GD2202 from Mosquitoes in Foshan, China. Deng, Ying-Ling,Lin, Zi-Yi,Lin, Rui,Lu, Zi-Shuo,Yan, Xing-Xiu,Li, Li-Xia,Lu, Hui-Jun,Bai, Xue,Jin, Ning-Yi,Liu, Hao. 2025

[4]Virome in the cloaca of wild and breeding birds revealed a diversity of significant viruses. Tongling Shan,Shixing Yang,Haoning Wang,Hao Wang,Ju Zhang,Ga Gong,Yuqing Xiao,Jie Yang,Xiaolong Wang,Juan Lu,Min Zhao,Zijun Yang,Xiang Lu,Ziyuan Dai,Yumin He,Xu Chen,Rui Zhou,Yuxin Yao,Ning Kong,Jian Zeng,Kalim Ullah,Xiaochun Wang,Quan Shen,Xutao Deng,Jianmin Zhang,Eric Delwart,Guangzhi Tong,Wen Zhang. 2022

[5]Metagenomic analysis of viral diversity and a novel astroviruse of forest rodent. Yin H.-C.,Wan D.-C.,Chen H.-Y.. 2022

[6]Comparison of viral communities in the blood, feces and various tissues of wild brown rats (Rattus norvegicus). Zi Zhuang,Lingling Qian,Juan Lu,Xiaodan Zhang,Asif Mahmood,Lei Cui,Huiying Wang,Xiaochun Wang,Shixing Yang,Likai Ji,Tongling Shan,Quan Shen,Wen Zhang. 2023

[7]The multi-kingdom microbiome of the goat gastrointestinal tract. Yanhong Cao,Tong Feng,Yingjian Wu,Yixue Xu,Li Du,Teng Wang,Yuhong Luo,Yan Wang,Zhipeng Li,Zeyi Xuan,Shaomei Chen,Na Yao,Na L. Gao,Qian Xiao,Kongwei Huang,Xiaobo Wang,Kuiqing Cui,Saif Ur Rehman,Xiangfang Tang,Dewu Liu,Hongbing Han,Ying Li,Wei Hua Chen,Qingyou Liu. 2023

[8]Improving the safety and efficacy of phage therapy from the perspective of phage-mammal interactions. Geng Zou,Lijun He,Jing Rao,Zhiyong Song,Hu Du,Runze Li,Wenjing Wang,Yang Zhou,Lu Liang,Huanchun Chen,Jinquan Li. 2023

[9]Meta-transcriptomic analysis of companion animal infectomes reveals their diversity and potential roles in animal and human disease. Wu, Wei-Chen,Pan, Yuan-Fei,Zhou, Wu-Di,Liao, Yu-Qi,Peng, Min-Wu,Luo, Geng-Yan,Xin, Gen-Yang,Peng, Ya-Ni,An, Tongqing,Li, Bo,Luo, Huanle,Barrs, Vanessa R.,Beatty, Julia A.,Holmes, Edward C.,Zhao, Wenjing,Shi, Mang,Shu, Yuelong. 2024

[10]Thrips and plant viruses: a comprehensive virome analysis in tropical agriculture. Qikai Zhang,Chunxi Cheng,Baoqian Lyu,Yulin Gao,Lilin Chen,Liang Peng,Zhengfu Yue,Hui Lu,Jihong Tang,Bin Jiao,Shen Liu. 2025

[11]Isolation and complete nucleotide sequence of a Batai virus strain in Inner Mongolia, China. Liu, Hao,Shao, Xi-qun,Hu, Bo,Zhao, Jian-jun,Zhang, Lei,Zhang, Hai-ling,Bai, Xue,Zhang, Run-xiang,Niu, Deng-yun,Sun, Yan-gang,Yan, Xi-jun,Niu, Deng-yun. 2014

[12]A potentially novel reovirus isolated from swine in northeastern China in 2007. Zhang, Chaofan,Liu, Shanshan,Lin, Wencheng,Hu, Feng,Cui, Shangjin,Liu, Licheng,Wang, Pengzhi,Chen, Weijun,Wu, Weili,Chen, Weijun. 2011

[13]Pathogenicity of modified bat influenza virus with different M genes and its reassortment potential with swine influenza A virus. Yang, Jianmei,Lee, Jinhwa,Ma, Jingjiao,Lang, Yuekun,Nietfeld, Jerome,Li, Yuhao,Duff, Michael,Li, Yonghai,Yang, Yuju,Liu, Haixia,Richt, Juergen A.,Ma, Wenjun,Yang, Jianmei,Li, Zejun,Zhou, Bin,Wentworth, David E.,Zhou, Bin,Wentworth, David E..

[14]Isolation and genetic analysis of human origin H1N1 and H3N2 influenza viruses from pigs in China. Yu, Hai,Zhang, Gui-Hong,Hua, Rong-Hong,Zhang, Qiang,Liu, Tian-Qiang,Liao, Ming,Tong, Guang-Zhi.

[15]Two genotypes of H1N2 swine influenza viruses appeared among pigs in China. Xu, Chuantian,Zhu, Qiyun,Yang, Huanliang,Qiao, Chuanling,Chen, Yan,Xin, Xiaoguang,Chen, Hualan,Xu, Chuantian,Zhu, Qiyun,Yang, Huanliang,Qiao, Chuanling,Chen, Yan,Xin, Xiaoguang,Chen, Hualan,Xu, Chuantian,Zhang, Xiumei. 2009

[16]Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats. Li, Zhijie,Liu, Dafei,Ran, Xuhua,Liu, Chunguo,Guo, Dongchun,Hu, Xiaoliang,Tian, Jin,Zhang, Xiaozhan,Shao, Yuhao,Liu, Shengwang,Qu, Liandong.

[17]A reassortment vaccine candidate as the improved formulation to induce protection against very virulent infectious bursal disease virus. Qi, Xiaole,Chen, Yuming,Ren, Xiangang,Zhang, Lizhou,Gao, Li,Wang, Nian,Qin, Liting,Wang, Yongqiang,Gao, Yulong,Wang, Xiaomei. 2014

[18]Genetic and biological characterization of two novel reassortant H5N6 swine influenza viruses in mice and chickens. Li, Xuyong,Fu, Yuguang,Yang, Jiayun,Guo, Jing,He, Jijun,Guo, Jianhong,Weng, Shaoting,Jia, Yane,Liu, Bin,Li, Xiaoyang,Zhu, Qiyun,Chen, Hualan. 2015

[19]The PB1 gene from H9N2 avian influenza virus showed high compatibility and increased mutation rate after reassorting with a human H1N1 influenza virus. Hongrui Cui,Guangsheng Che,Mart C.M. de Jong,Xuesong Li,Qinfang Liu,Jianmei Yang,Qiaoyang Teng,Zejun Li,Nancy Beerens. 2022

[20]Genome-Wide Reassortment Analysis of Influenza A H7N9 Viruses Circulating in China during 2013–2019. Dongchang He,Xiyue Wang,Huiguang Wu,Xiaoquan Wang,Yayao Yan,Yang Li,Tiansong Zhan,Xiaoli Hao,Jiao Hu,Shunlin Hu,Xiaowen Liu,Chan Ding,Shuo Su,Min Gu,Xiufan Liu. 2022

作者其他论文 更多>>